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2 protocols using ethanol lc ms grade

1

Antioxidant Capacity Assay Protocol

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[2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)]-diammonium salt (ABTS), Folin–Ciocalteu’s reagent, 6-hydroxy-2, 5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), gallic acid (GA), potassium chloride, potassium persulfate, 2,4, 6- tripyridyl-S-triazine (TPTZ) and iron (III) chloride hexahydrate (FeCl3 6H2O) were purchased from Sigma-Aldrich. Ethanol (LC-MS grade) was purchased from Biosolve B.V. (Valkenswaard, The Netherlands). All other materials and solvents were of analytical grade.
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2

Lipid Analysis by Mass Spectrometry

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2,5-Dihydroxyacetophenone (DHA, 97% purity),
sodium acetate (anhydrous, >99%), and chloroform (≥99%)
were
purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands) and used
without further purification. Methanol (ULC-MS grade), water (LC-MS
grade), ethanol (LC-MS grade), and xylene (AR grade) were purchased
from Biosolve (Valkenswaard, The Netherlands). Hematoxylin and Entellan
were purchased from Merck (Darmstadt, Germany) and eosin Y from J.T.
Baker (Center Valley). Indium tin oxide (ITO)-coated glass slides
were purchased from Delta Technologies (Loveland). Lipid standards
(PC 18:0/18:1n-9 and PC 16:0/18:1n-9) were purchased from Avanti Polar Lipids (Alabaster).
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