Human isg15 7amino 4 methylcourmarin isg15 amc

Manufactured by R&D Systems

ISG15-AMC is a fluorogenic substrate that can be used to detect and measure the activity of the enzyme ISG15 protease. ISG15 is a ubiquitin-like protein that plays a role in the innate immune response. The AMC (7-amino-4-methylcoumarin) moiety of the substrate is cleaved by ISG15 protease, resulting in the release of a fluorescent signal that can be measured.

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Lab products found in correlation

Imidazole, by Thermo Fisher Scientific (2 mentions) Z rlrgg 7 amino 4 methyl courmarin peptide amc, by Bachem (2 mentions) Ubiquitin 7amino 4 methylcourmarin ub amc, by R&D Systems (2 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Thermo Fisher Scientific (2 mentions) Tris hydroxymethyl aminomethane tris, by Thermo Fisher Scientific (2 mentions) Dl dithiothroitol dtt, by GoldBio (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Losartan, by Thermo Fisher Scientific (1 mentions) Lys48 linked tetra ub, by R&D Systems (1 mentions) Isopropyl β d thiogalactopyranoside, by GoldBio (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Ampicillin, by GoldBio (1 mentions) Linear linked di ub, by R&D Systems (1 mentions) Dehydrated luria bertani broth, by Thermo Fisher Scientific (1 mentions) Isopropyl β d thiogalactopyranoside iptg, by GoldBio (1 mentions)

2 protocols using human isg15 7amino 4 methylcourmarin isg15 amc

Ubiquitin and ISG15 Substrate Assay

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Z-RLRGG-7-amino-4-methyl-courmarin (peptide-AMC) was purchased from Bachem. Ubiquitin-7amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15-7amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem. Lys48 linked tetra-Ub were obtained from Boston Biochem; DLdithiothroitol (DTT) was purchased from GoldBio, and isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from GoldBio. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from Fisher BioReagents. Imidazole was purchased from Acros Organics; tris(hydroxymethyl)aminomethane (Tris) was purchased from Fisher Scientific. Losartan and Sodium chloride (NaCl) were purchased from Fisher Chemical and bovine serum albumin (BSA) was purchased from Sigma Life Science.

Nejat R., Sadr A.S., Freitas B., Crabttree J., Pegan S.D., Tripp R.A, & Najafi D. (2021). Losartan Inhibits SARS-CoV-2 Replication in Vitro. Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 24.

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Recombinant Protein Expression and Purification

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5-Amino-2-methyl-N-[(R)-1-(1-naphthyl)ethyl]benzamide (GRL0617) was purchased from Raystar, CN; 5-(acetylamino)-2-methyl-N-[(1R)-1-(1-napthanlenyl)ethyl]-benzamide (compound 6) was purchased from MedChem Express; Z-RLRGG-7-amino-4-methyl-courmarin (peptide-AMC) was purchased from Bachem; Ubiquitin–7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15-7-amino-4-methylcourmarin (ISG15-AMC) was purchased from Boston Biochem; Lys6, Lys11, Lys29, Lys33, Lys48, Lys63, and linear linked di-Ub were obtained from Boston Biochem; DL-dithiothroitol (DTT) was purchased from GoldBio; Isopropyl β-D-thiogalactopyranoside (IPTG) was purchased from GoldBio; 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from Fisher BioReagents; Imidazole was purchased from Acros Organics; tris(hydroxymethyl)aminomethane (Tris) was purchased from Fisher Scientific; Sodium chloride (NaCl) was purchased from Fisher Chemical; Bovine serum albumin (BSA) was purchased from Sigma Life Science; Dehydrated Luria-Bertani (LB) broth was purchased from Fisher Scientific; Ampicillin was purchased from GoldBio.

Freitas B.T., Ahiadorme D.A., Bagul R.S., Durie I.A., Ghosh S., Hill J., Kramer N.E., Murray J., O’Boyle B.M., Onobun E., Pirrone M.G., Shepard J.D., Enos S., Subedi Y.P., Upadhyaya K., Tripp R.A., Cummings B.S., Crich D, & Pegan S.D. (2022). Exploring noncovalent protease inhibitors for the treatment of SARS and SARS-like coronaviruses. ACS infectious diseases, 8(3), 596-611.

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