Dpp gal4

Manufactured by BD

Sourced in Morocco

Dpp-GAL4 is a genetic tool used in research laboratories. It functions as a driver that can selectively express target genes in specific cell types or tissues. The core function of Dpp-GAL4 is to facilitate the controlled expression of genes of interest in Drosophila melanogaster (fruit fly) models.

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Lab products found in correlation

Uas bskdn, by BD (1 mentions) Gmr gal4, by BD (1 mentions) Apgal4, by BD (1 mentions) En gal4, by BD (1 mentions) Ptc gal4, by BD (1 mentions) C96 gal4, by BD (1 mentions) Mef2 gal4, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using dpp gal4

Drosophila Genetics and Imaging Protocols

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All fly stocks were maintained on standard cornmeal/yeast/molasses/agar medium at 25 °C as per standard procedures. Oregon-R flies were used as wild-type controls. UAS-GFP-zip, UAS-GFP-Zip DN (UAS-Myo II-Neck-Rod), UAS-Myo II-Rod, and UAS-Myo II-Rod (delta N_term58) [26 (link)] were obtained as a gift from Prof. Daniel P. Kiehart (Department of Biology, Duke University, Durham, NC). UAS-Notch-FL [27 (link)], UAS-Notch-ICD, UAS-Notch-DN [28 (link)], and Notch pathway components were kindly provided by Prof. S. Artavanis-Tsakonas (Department of Cell Biology, Harvard Medical School, Boston, MA).

cn¹ bw¹ sp¹ zip¹/CyO (BDSC 4199),

P{FRT(w^hs)}G13 zip²/CyO (BDSC 8739),

P{lacZ.w⁺}276, y¹ sc* v¹ sev²¹; P{TRiP.GL00623}attP40 (BDSC 37480), y¹ sc* v¹ sev²¹; P{TRiP.HMS01618}attP2 (BDSC 65947),

Sqh^AX3-GFP (BDSC 57144), UAS-Bsk-DN (BDSC 6409), vg-GAL4 (BDSC 8222), GMR-GAL4 (BDSC 8121), ptc-GAL4 (BDSC 2017), en-GAL4 (BDSC 30564), ap-GAL4 (BDSC 56807), dpp-GAL4 (BDSC 1553), and C96-GAL4 (BDSC 43343) stocks were obtained from Bloomington stock center. All crosses were performed at 25 °C. The combination lines vg-GAL4/UAS-GFP-zip and UAS-Notch-DN/C96-GAL4 were made with the help of appropriate genetic crosses.

Verma D., Singh A., Singh J., Mutsuddi M, & Mukherjee A. (2024). Regulation of Notch signaling by non-muscle myosin II Zipper in Drosophila. Cellular and Molecular Life Sciences: CMLS, 81(1), 195.

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Drosophila Genetics and Developmental Biology

Cited 2 times

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Flies were grown and crossed on standard food containing cornmeal, molasses, and yeast at room temperature (~22°C). The following fly strains were used in this study: Mef2-GAL4 (Bloomington Drosophila Stock Center (BDSC) #27390), UAS-CD8::GFP, UAS-lacZ, UAS-dpp-GFP (BDSC #53716) (Teleman and Cohen, 2000 (link)), dpp-GAL4 (BDSC #1553), Mi{MIC}dpp^MI03752 (BDSC #36399) PBac{RB}e00178 (Exelixis at Harvard Medical School), Pngl^ex14 (Funakoshi et al., 2010 (link)), UAS-Pngl^RNAi KK101641 (Vienna Drosophila Resource Center), UAS-attB-NGLY1^WT-VK31 (Galeone et al., 2017 (link)), UAS-attB-NGLY1^N41P-VK31, UAS-attB-NGLY1^F70A/G80A-VK31, dpp^HA and dpp^HA-3NQ (this study). For survival (eclosion test), the expected ratio of offspring was calculated based on Mendelian inheritance for each genotypic class and the observed/expected ratio is reported as a percentage.

Galeone A., Adams J.M., Matsuda S., Presa M.F., Pandey A., Han S.Y., Tachida Y., Hirayama H., Vaccari T., Suzuki T., Lutz C.M., Affolter M., Zuberi A, & Jafar-Nejad H. (2020). Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation. eLife, 9, e55596.

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