Silica gel g coated tlc plates

Methanol (Merck, Mumbai, India) was used as a solvent in the Shimadzu-120 double beam spectrophotometer to determine Ultraviolet spectra. Shimadzu FTIR-8400 was used to detect and document IR spectra in KBr pellets. Scanning of ¹H NMR (300 MHz) and ¹³C NMR (75 MHz) spectra was carried out using the Bruker spectrospin NMR apparatus exploiting TMS as the internal standard. EIMS at 70 eV with a Jeol D-300 device (Jeol, USA) was used for scanning, and silica gel (60–120 mesh, Merck, Mumbai, India) was used for column chromatography. For thin-layer chromatography, silica gel G-coated TLC plates manufactured by Merck in Mumbai, India, were used. For thin-layer chromatography, silica gel G-coated TLC plates (Merck, Mumbai, India) were employed. Based on the dried plant material acquired for extraction (4.8 kg), an estimate of the % yields of the isolated compounds were determined.