Petunia volatiles were collected from infiltrated flowers by a closed-loop stripping method and analyzed by GC-MS (Widhalm et al., 2015 ). Briefly, floral scents were absorbed in VOC collection traps containing 20 mg of Porapak Q (80–100 mesh) matrix (Waters). VOCs were eluted from scent collection traps with 200 μl of dichloromethane. A 1 μg aliquot of naphthalene was added to the eluted sample as an internal standard. Samples were analyzed on an Agilent 6890N-5975B GC-MS system. Quantitation of different volatile compounds was performed based on standard curves generated with commercially available standards.
Porapak q 80 100 mesh matrix
Manufactured by Waters Corporation
Porapak Q (80–100 mesh) is a porous polymer matrix used as a stationary phase in chromatographic separations. It is designed to provide efficient separation and purification of a wide range of organic compounds. The matrix consists of a highly cross-linked, macroporous polymer structure with a specific surface area and pore size distribution that enables effective adsorption and desorption of target analytes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using porapak q 80 100 mesh matrix
1
Petunia Floral Volatiles Analysis
2
Petunia Floral Scent Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Petunia volatiles were collected from infiltrated flowers by a closed-loop stripping method and analyzed by GC-MS (Widhalm et al., 2015) . Briefly, floral scents were absorbed in VOC collection traps containing 20 mg of Porapak Q (80-100 mesh) matrix (Waters). VOCs were eluted from scent collection traps with 200 μl dichloromethane. One microgram of naphthalene was added to the eluted sample as an internal standard. Samples were analyzed on an Agilent 6890N-5975B GC-MS system. Quantitation of different volatile compounds was performed based on standard curves generated with commercially available standards.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!