ChIP experiments were performed using 1 × 107 pancreatic cancer cells with the ChIP kit (Cell Signaling Technology) according to the manufacturer’s protocol. Briefly, PDAC cells were cross-linked with 1% formaldehyde. Then the cells were lysed and chromatin was harvested and fragmented using enzymatic digestion. The chromatin was immunoprecipitated using ChIP-grade antibody against ZEB1 (Sigma, United States). After immunoprecipitation, the protein-DNA cross-links were reversed and the DNA was purified, followed by qRT-PCR. The enrichment was calculated using the following formula: percentage = 2% × 2(C[T]Input Sample–C[T]IP Sample). The qRT-PCR products were used for DNA electrophoresis and visualized by ethidium bromide staining.
Chip grade antibody against zeb1
Manufactured by Merck Group
Sourced in United States
The ChIP-grade antibody against ZEB1 is a laboratory tool designed for chromatin immunoprecipitation (ChIP) experiments. It is a high-quality antibody specifically targeting the ZEB1 protein, a key transcription factor involved in various cellular processes. The antibody is suitable for ChIP applications to study the binding of ZEB1 to genomic regions, providing researchers with a valuable tool for investigating gene regulation and transcriptional networks.
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2 protocols using chip grade antibody against zeb1
1
ChIP-qPCR Analysis of ZEB1 in PDAC
2
ChIP-qPCR Assay for ZEB1 Enrichment
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ChIP experiments were performed using 1 × 10 7 pancreatic cancer cells with the ChIP kit (Cell Signaling Technology) according to the manufacturer's protocol. Brie y, PDAC cells were cross-linked with 1% formaldehyde. Then the cells were lysed and chromatin was harvested and fragmented using enzymatic digestion. The chromatin was subjected to immunoprecipitation using ChIP-grade antibody against ZEB1 (Sigma, USA). After immunoprecipitation, the protein-DNA cross-links were reversed and the DNA was puri ed, followed by qRT-PCR. The enrichment percentage = 2% × 2 (C[T] Input Sample -C [T] IP Sample )
. The qRT-PCR products were used for DNA electrophoresis and visualized by ethidium bromide staining.
. The qRT-PCR products were used for DNA electrophoresis and visualized by ethidium bromide staining.
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