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Sorafenib tosylate

Manufactured by LC Laboratories
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Sorafenib tosylate is a chemical compound produced by LC Laboratories. It is a white to off-white crystalline powder.

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Lab products found in correlation

Dasatinib, by LC Laboratories (2 mentions) Octadecylamine, by Merck Group (1 mentions) N docosane, by Merck Group (1 mentions) β benzyl l aspartate, by Merck Group (1 mentions) Lactide co glycolide, by Merck Group (1 mentions) 1 3 aminopropyl imidazole api, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) 1 octadecene, by Merck Group (1 mentions) Dichloromethane, by Merck Group (1 mentions) N hexane, by Merck Group (1 mentions) Tetrahydrofuran, by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Iron 3 chloride hexahydrate, by Merck Group (1 mentions) N n dimethylformamide (dmf), by Merck Group (1 mentions) Diethyl ether, by Merck Group (1 mentions) Sodium oleate, by Tokyo Chemical Industry (1 mentions) Triphosgene, by Tokyo Chemical Industry (1 mentions) Nutlin 3a, by Selleck Chemicals (1 mentions)

7 protocols using sorafenib tosylate

1

Synthesis and Characterization of Multifunctional Nanoparticles

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All reagents and solvents were obtained commercially and used without further purification. Poly(D,L-lactide-co-glycolide) (lactide:glycolide 50:50, PLGA), poly(vinyl alcohol) (Mw 89 000–98 000, 99+% hydrolyzed, PVA), β-benzyl-L-aspartate (BLA), 1-(3-aminopropyl)imidazole (API), octadecylamine, iron(III) chloride hexahydrate (≥98%), N,N-dimethylformamide (DMF), tetrahydrofuran (THF), diethyl ether (≥99.9%), dichloromethane (CH2Cl2), chloroform (CHCl3), dimethyl sulfoxide (DMSO), methanol, n-docosane (99%), 1-octadecene (90%), n-hexane, and ethanol were purchased from Sigma–Aldrich Co (Milwaukee, WI). Sodium oleate (>97%) and triphosgene (98%) were purchased from TCI America (Portland, OR). Sorafenib tosylate was purchased from LC Laboratories (Woburn, MA). Iron oleate was prepared by following a reported procedure.35 (link)β-Benzyl-L-aspartate N-carboxy anhydride (BLA-NCA) was synthesized by following our previous report.15 (link),33 (link)
Park W., Chen J., Cho S., Park S.J., Larson A.C., Na K, & Kim D.H. (2016). Acidic pH-Triggered Drug-Eluting Nanocomposites for Magnetic Resonance Imaging-Monitored Intra-arterial Drug Delivery to Hepatocellular Carcinoma. ACS applied materials & interfaces, 8(20), 12711-12719.
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2

Sorafenib Tosylate API Validation

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Sorafenib tosylate was obtained from Diamondback Drugs (Scottsdale, AZ, USA). The active pharmaceutical ingredient (API) was validated for identity by the International Analytical Laboratory, in Castle Rock, CO, USA. The active ingredient was assayed using ultrahigh-performance liquid chromatography (UHPLC). This instrument was fitted with a photodiode array having a C18 column and a mobile phase of trifluoroacetic acid and acetonitrile. The reference standard of Sorafenib tosylate was obtained from LC Laboratories (Woburn, MA, USA). The identity of the sample was confirmed by matching the retention time and ultraviolet (UV) spectrum with that of the reference standard. The tested Sorafenib tosylate API powder was determined to be 99% potent.
Foskett A., Manley C., Naramore R., Gordon I.K., Stewart B.M, & Khanna C. (2017). Tolerability of oral sorafenib in pet dogs with a diagnosis of cancer. Veterinary Medicine : Research and Reports, 8, 97-102.
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3

Sorafenib Formulations and shRNA Knockdown

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Sorafenib base and sorafenib tosylate were purchased from LC Laboratories (Woburn, MA, USA). For the in vitro study, sorafenib base was dissolved in dimethyl sulfoxide (DMSO). To prepare the stock solutions for the in vivo study, sorafenib tosylate was dissolved in Cremophor EL/ethanol (50:50, v/v) at 4× concentration and then diluted with sterile water to 1× concentration. MISSION® shRNA lentiviral transduction particles containing the shRNA sequence targeting the coding region of HTATIP2 gene (TRCN0000280445) and MISSION® TRC2 pLKO.5-puro non-target shRNA control transduction particles (Catalog number: SHC216V) were purchased from Millipore Sigma (St. Louis, MO, USA). All other chemicals, solvents and reagents were obtained from commercial sources.
Li M., Li J., Guo X., Pan H, & Zhou Q. (2020). Absence of HTATIP2 Expression in A549 Lung Adenocarcinoma Cells Promotes Tumor Plasticity in Response to Hypoxic Stress. Cancers, 12(6), 1538.
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4

Sorafenib-Loaded PLGA Nanoparticles

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75:25 Poly (D,L-lactide-co-glycolide) (PLG RESOMER® RG 752H, MW=4000–15000) polymer was purchased from Sigma Aldrich (St. Louis, MO). Sorafenib tosylate was purchased from LC Laboratories (Woburn, MA). The iron oxide was in a ferrofluid solution (EMG 304) and was purchased from Ferrotec (Santa Clara, CA).
Chen J., White S.B., Harris K.R., Li W., Yap J.W., Kim D.H., Lewandowski R.J., Shea L.D, & Larson A.C. (2015). Poly(lactide-co-glycolide) Microspheres for MRI-Monitored Delivery of Sorafenib in a Rabbit VX2 model. Biomaterials, 61, 299-306.
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5

Evaluating Synergistic Drug Combinations

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PD0325901, sorafenib tosylate, dasatinib (LC Laboratories, Woburn, MA, USA), rapamycin doxorubicin, Nutlin-3a, SGX-523 (Selleck, Houston, TX, USA), and KT5270 (Santa Cruz Biotechnology, Dallas, TX, USA) were prepared in DMSO (Sigma-Aldrich, St. Louis, MO, USA). Cells were seeded into a 96-well plate using an epMotion 5075 pipetting system (Eppendorf, Hamburg, Germany). Treatments began when cells reached 30% confluency. Cells were treated for 72 h. Cell viability was measured using the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Assays were measured using a Benchmark Plus microplate spectrophotometer (Bio-Rad, Hercules, CA, USA) at 490 and 700 nm reference wavelengths. Cell viability was performed twice in duplicate wells. Treated wells were normalized to non-treated wells and then were normalized to DMSO-treated plates. The concentration of compound required to cause 50% inhibition of cell viability (IC50) was calculated (23 (link)). A combinatorial index was calculated following Chou and Talalay (23 (link)). If a drug combination resulting in synergy (CI<1) that combination was repeated for a total of three separate experiments in duplicate wells. The IC50 and CI were then calculated as previously described (23 (link)).
ANDERSEN N.J., BOGUSLAWSKI E.B., KUK C.Y., CHAMBERS C.M, & DUESBERY N.S. (2015). Combined inhibition of MEK and mTOR has a synergic effect on angiosarcoma tumorgrafts. International Journal of Oncology, 47(1), 71-80.
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6

Sorafenib Mechanism of Action

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Sorafenib tosylate was purchased from LC Laboratories (Boston, MA) and was dissolved in DMSO. Cycloheximide (CHX) and MG132 were obtained from Sigma Aldrich and dissolved in ethanol and DMSO, respectively. Antioxidant MnTBAP (Mn(III) tetrakis (4-benzoic acid) porphyrin chloride) was purchased from Merck Millipore. Antibodies for AIB1 (5E11), eIF4E, phospho-eIF4E (Ser209), mTOR, phosphor-mTOR(Ser2448), p70S6K, phospho-p70S6K (Thr389), RP-S6, phospho-RP-S6 (Ser235/236), 4EBP1, phospho-4E-BP1 (Ser65) were purchased from Cell Signaling Technologies. Antibodies for PARP, Mcl-1 were from Santa Cruz Biotechnology. β-actin antibody was purchased from Sigma.
Li M., Wang W., Dan Y., Tong Z., Chen W., Qin L., Liu K., Li W., Mo P, & Yu C. (2016). Downregulation of amplified in breast cancer 1 contributes to the anti-tumor effects of sorafenib on human hepatocellular carcinoma. Oncotarget, 7(20), 29605-29619.
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7

Reagents for Cancer Cell Studies

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Imatinib, gefitinib, erlotinib hydrochloride, sorafenib tosylate, sunitinib malate, dasatinib, lapatinib, nilotinib, pazopanib hydrochloride, axitinib, and afatinib were purchased from LC laboratories (Woburn, MA, USA). Testosterone was purchased from Sigma–Aldrich (St. Louis, MO, USA). Acetonitrile, methanol, and water (LC–MS grade) were purchased from EMD (Gibbstown, NJ, USA). Formic acid was purchased from Fisher Chemical (Pittsburgh, PA, USA). DMSO was purchased from Corning (Manassas, VA, USA). All other chemicals were used as received.
He Y., Zhou L., Gao S., Yin T., Tu Y., Rayford R., Wang X, & Hu M. (2017). Development and validation of a sensitive LC–MS/MS method for simultaneous determination of eight tyrosine kinase inhibitors and its application in mice pharmacokinetic studies. Journal of pharmaceutical and biomedical analysis, 148, 65-72.
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