Ultimate splashone

Manufactured by Avanti Polar Lipids

The Ultimate SplashOne is a laboratory centrifuge designed for rapid and efficient separation of samples. Its core function is to provide controlled centrifugal force to separate components of a liquid mixture based on their density and size.

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Lab products found in correlation

Equisplash, by Avanti Polar Lipids (1 mentions) Milli q purification system, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions) Acetonitrile, by Honeywell (1 mentions)

2 protocols using ultimate splashone

Comprehensive Lipid Profiling Standards

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All standards were purchased from Avanti Polar Lipids: Ultimate SplashOne (#330820), dFA 18:1 (#861809), dFA 20:4 (#861810), dCer d18:0/13:0 (#330726), Glu Cer(d18:1-d7/15:0) (#330729), dLacCer d18:1/15:0 (#330727), 15:0-18:1-d7-PA (#791642), EquiSPLASH (#330731).

Shashikadze B., Valla L., Lombardo S.D., Prehn C., Haid M., Riols F., Stöckl J.B., Elkhateib R., Renner S., Rathkolb B., Menche J., Hrabĕ de Angelis M., Wolf E., Kemter E, & Fröhlich T. (2023). Maternal hyperglycemia induces alterations in hepatic amino acid, glucose and lipid metabolism of neonatal offspring: Multi-omics insights from a diabetic pig model. Molecular Metabolism, 75, 101768.

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Lipidomic Analysis with Deuterated Standards

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Acetonitrile, dichloroform (DCM), isopropanol, and methanol (MEOH) were purchased from Honeywell (NJ). All solvents were HPLC grade. Deionized water was provided by a Millipore Milli-Q purification system (Bedford, MA). Ammonium acetate was purchased from Sigma-Aldrich (Taufkirchen, Germany). The authentic standards of triacylglycerol (TAG) including tritridecanoin (C13:0, CAS#26536-12-9), tripentadecanoin (C15:0, CAS#7370-46-9), triheptadecanoin (C17:0, CAS#2438-40-6), and tritricosanoin (C23:0, CAS#86850-72-8) were bought from Shanghai ANPEL Experimental Technology Co, Ltd (https://www.labsci.com.cn/). UltimateSPLASH™ ONE (catalog no.: 330820L) for lipidomic analysis is a commercialized mixture of deuterium-labeled internal standard (IS) provided by Avanti Polar Lipids, Inc (AL). The mixture consists of the 69 deuterium-labeled ISs in 1:1 dichloromethane:MEOH solution (1.2 ml per vial). Detailed ISs are listed in supplemental Table S1. The isotope-labeled standards were provided at defined concentrations by Avanti Polar Lipids and used without further purification or validation. Certificate of analysis for UltimateSPLASH™ ONE is available at https://avantilipids.com/.

Zhang J., Yang S., Wang J., Xu Y., Zhao H., Lei J., Zhou Y., Chen Y., Wu L, & Li Y. (2023). Equivalent carbon number-based targeted odd-chain fatty acyl lipidomics reveals triacylglycerol profiling in clinical colon cancer. Journal of Lipid Research, 64(7), 100393.

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