I2=100%×(Q–df)/Q
, where Q is Cochran’s heterogeneity statistic and df is the degrees of freedom. Negative values of I2 are set at zero; I2 ranges from 0% (no heterogeneity) to 100% (maximal heterogeneity). If I2 value is greater than 50%, it represents the substantial heterogeneity [25 ]. Because individual studies were conducted in different populations, the random-effects model with the DerSimonian and Laird method was used to calculate the pooled effect size [26 (link),27 (link)].
Publication bias was assessed by using the Begg’s funnel plot and Egger’s test [28 (link)]. If the funnel plot is asymmetric or the p-value for Egger’s test is lower than 0.05, there exists publication bias. The Stata SE ver. 14.0 software (StataCorp., College Station, TX) was used for all statistical analyses.