Brie y, cells were rst evenly grown in 12-well plates, collected after drug treatment, resuspended in ow tubes (50,000 cells/tube), incubated with 2 µl of PI reagent (KeyGEN BioTECH, China) for 10 min and analyzed for dead cells using a ow cytometer (BECKMEN COULTER, USA).
Pi reagent
Manufactured by Keygen Biotech
Sourced in China
The PI reagent is a laboratory product used for cell analysis. It serves as a dye that binds to cellular nucleic acids, allowing for the quantification and identification of cells. The PI reagent provides a means to measure cellular parameters, but its specific applications should be determined by the user based on their research requirements.
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Lab products found in correlation
4 protocols using pi reagent
1
Flow Cytometric Analysis of Cell Death
2
Cell Cycle Analysis of OCI-Ly8 Cells
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OCI-Ly8 cells were plated onto 6-well plates overnight at 37°C, and then fixed with 70% ethanol for 24 h at 4°C. After that, cells were incubated with PI reagent (50 μg/ml; KeyGen BioTECH) in darkness for 30 min. Subsequently. the distribution of cell cycle phases was evaluated by a FACSCalibur Flow Cytometer (BD Biosciences).
3
Flow Cytometric Analysis of RAW264.7 Apoptosis
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The apoptosis of RAW264.7 cells was analysed using the flow cytometry assay. The RAW264.7 cells (1 × 105 cells/mL) were collected in a 15 mL tube after treatment. Following staining with Annexin V-APC and propidium iodide (PI) reagent (KeyGen BioTech, China) for 15 min at room temperature in dark and the cell apoptosis was rapidly analysed using a FSCAN flow cytometer (BD Biosciences, USA).
4
Cell Cycle and Apoptosis Analysis
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SW620 cells were scattered in 6-well plates, then coped with diverse concentrations of Tet for 48 h. In terms of cell cycle analysis, cells were gathered, washed with cold phosphate-buffered saline (PBS) and immobilized with cold ethanol(4 °C, 70%). Finally, fluorescence-activated cell sorting (FACS) was used to detect cells, which were stained for 30 min by PI. In terms of apoptosis analysis, cells were gathered and washed with cold phosphate-buffered saline (PBS), followed by coping with Annexin V-EGFP and PI reagent from Nanjing KeyGen Biotech Company in China. Finally, the cells were detected with fluorescence-activated cell sorting (FACS). Per assay was done three times.
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