Caki 1 htb 46

Six‐ to eight‐week‐old NOD.CB17‐Prkdc^scid/J mice (Jackson Laboratory, Bar Harbor, ME, USA) were housed in a pathogen‐free facility at Baylor College of Medicine. The NES‐p27 and phosphosite mutants used in this study were previously reported (Ahmed et al., 2009; Li et al., 2016). The Caki‐1 (HTB‐46) and MDA‐MB‐468 (HTB‐132) cell lines were purchased from American Type Culture Collection. HT‐1080 was a gift from N. Ahmed.

Two renal cancer cell lines Caki-1 (HTB-46) and Caki-2 (HTB-47), obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA), were used for the study. Testing confirmed that the cells were not contaminated with mycoplasma. All the experiments were performed using cells with passage number ≤5. The cell characteristics, as provided by ATCC, are given in Supplementary Table S1. Three independent biological experiments were performed using the two cell lines. The cells were cultured in McCoy’s 5A Media containing 10% fetal bovine serum, 1× penicillin/streptomycin, 1 mM sodium pyruvate, and 25 mM HEPES buffer at 37 °C in 5% CO₂.