Mrsa mu50

Staphylococcus epidermidis ATCC 35984 and ATCC 14990, S. aureus ATCC 25923 and ATCC 700699 (also known as MRSA Mu50), and Escherichia coli ATCC 25922 were purchased from the American Type Culture Collection (Manassas, VA, United States). Three clinical isolates, i.e., MRSA 1, 2 and 3 were obtained from Adelaide Pathology Partners (Mile End, Australia). Pseudomonas aeruginosa PAO1 was obtained from the School of Molecular Medical Sciences, University of Nottingham (Nottingham, United Kingdom). Unless stated otherwise, bacterial suspensions were prepared by dissolving colonies in 0.9% saline and adjusted to the appropriate McFarland units before being further diluted in broth and incubated at 37°C under aerobic conditions. Cell culture studies were carried out using control human fibroblast cells (Coriell Cat# GM00038, RRID: CVCL_7271) obtained from the Coriell Institute for Medical Research (Camden, NJ, United States). Unless stated otherwise, all experiments were carried out at least in triplicate and all chemicals, media and supplements were purchased from Sigma-Aldrich (Steinheim, Germany).

S. aureus ATCC 6538, S. aureus ATCC 700699 (also known as MRSA Mu50) and S. epidermidis ATCC 35984 were purchased from the American Type Culture Collection (Manassas, VA, USA). Bacteria were inoculated at colony forming unit (CFU)/ml or optical density at 600 nm (OD₆₀₀) values stated after dilution of an overnight culture grown in tryptone soya broth (TSB) or nutrient broth (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C with shaking at 180 rpm. Tryptone soya agar (TSA) was prepared by adding 1.5% agar bacteriological (Thermo Fisher Scientific). The hernia meshes Parietex Hydrophilic 2-Dimensional mesh (polyester), Parietene Lightweight monofilament polypropylene mesh (polypropylene) were donated by Covidien (Dublin, Ireland). The saturated phospholipids 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycerol)-2000] (DSPE-mPEG2000) were donated by Lipoid GmbH (Ludwigshafen, Germany). Unless stated otherwise, all chemicals, materials, media and supplements were purchased from Sigma-Aldrich (Steinheim, Germany).