Au electrode (Φ = 2 mm) was purchased from Chenhua Instruments Co. (Shanghai, China). Acrylamide (AM, functional monomer), N, N′-methylene bis-acrylamide (MBA, cross-linker), ammonium persulfate, triethylamine (TEA), nickel sulfate, boric acid, ammonium chloride and sodium dodecylsulfate (SDS) were obtained from Kemiou Chemical Co. (Tianjin, China). Chloroauric acid (HAuCl4·4H2O) was purchased from Beijing chemical plant (Beijing, China). Mouse immunoglobulin G (IgG, MW 150 kDa), Human immunoglobulin M (IgM, MW 970 kDa), Human immunoglobulin A (IgA, MW 400 kDa), FITC-IgG, myoglobin (Mb, MW 16.7 kDa), human serum albumin (HSA, MW 69 kDa) and bovine hemoglobin (Hb, MW 65 kDa) were supplied by Solarbio Inc. (Beijing, China). Nickel chloride and saccharin were supplied from Aladdin Industrial Co. (Shanghai, China). Phosphate buffered solution (PBS) was prepared using 0.1 mol/L Na2HPO4 and 0.1 mol/L KH2PO4. All the chemicals were analytical reagent (AR) grade and used without any further purification. All the water used in the experiments was the hyperpure water (resistivity > 18 MΩ cm).
Bovine hemoglobin
Manufactured by Solarbio
Sourced in China
Bovine hemoglobin is a protein found in the red blood cells of cattle. It is a commonly used laboratory reagent for various applications in research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Human serum albumin (hsa), by Solarbio (1 mentions)
Trichloroacetic acid tca, by Merck Group (1 mentions)
Rosup, by Beyotime (1 mentions)
L methionine, by Merck Group (1 mentions)
Lc ms grade acetonitrile, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Dl homocysteine, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Catalase, by Worthington (1 mentions)
Hydrogen peroxide 30, by Merck Group (1 mentions)
Ficoll paque solution, by Solarbio (1 mentions)
2 protocols using bovine hemoglobin
1
Immunoassay Electrode Fabrication Protocol
2
Quantifying Homocysteine and Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DL-homocysteine, L-methionine, trichloroacetic acid (TCA), 2′,7′-dichlorofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). DL-homocysteine-3,3,4,4-d4 (homocysteine-d4) was obtained from CDN Isotopes Inc. (Quebec, Canada). L-homocysteine was from Bide Pharmatech Ltd. (Shanghai, China). Hydrogen peroxide (30%), LC-MS grade acetonitrile, and methanol were from Merck (Germany). Catalase was purchased from Worthington Biochemical Corporation (NJ, USA). 2-chloroquinoline, trimethyloxonium tetrafluoroborate, and formic acid were purchased from Aladdin (Shanghai, China). Tris-(2-carboxyethyl) phosphine hydrochloride (TCEP) was purchased from Energy Chemical (Shanghai, China). Glutathione was obtained from Generay Biotech Co. Ltd. (Shanghai, China), and 2-chloro-1-methylquinolinium tetrafluoroborate (CMQT) was synthesized according to the literature [20 (link)]. Rosup (a chemical mixture including 4-Butylhydroperoxide, 50 mg/mL in DMSO, catalog S0033S-2) was purchased from Beyotime Biotechnology (Shanghai, China). The Methemoglobin Assay Kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The bovine hemoglobin and Ficoll-Paque solution were from Beijing Solarbio Science and Technology Co. Ltd. (Beijing, China).
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