Accur c6 flow cytometer

Cells transfected with siRNA-LINC01447 or siRNA-AC106786.1 were seeded in 6-well plates and irradiated with 6 Gy of X-rays; they were then incubated for 48 h. For apoptosis assays, cells were trypsinized, washed, resuspended in 200 μL binding buffer, and then analyzed for apoptosis by double staining with 5 μL annexin V and 5 μL propidium iodide (Beyotime Biotechnology, China) and using a BD Accur™ C6 Flow Cytometer (BD Inc, Piscataway, NJ).

The HeLa cell line was used for cell apoptosis. Cells (20000 per mL)
were incubated with several concentrations of the compounds or drugs for
6–48 h, and incubated in DMEM medium supplemented with
12% fetal calf serum (FCS), 2 mM L -glutamine, and
100 mg/L penicillin G and 100 mg/L streptomycin at
37 °C and 5% CO₂. The cells were washed with
PBS twice, centrifuged at 206 g for 5 min, and
5–105 (link) cells were collected. Binding buffer
suspension (500 μL) was added to the cells, and then
5 μL of the FITC-Annexin V mix was added. Next,
10 μL of the PI mix was added, and the suspension was
mixed and kept at room temperature for 30 min in the dark. Analysis
was with a BD accur C6 flow cytometer.

The HeLa cell line was used for cell apoptosis. Cells (20000 per mL) were incubated with several concentrations of the compounds or drugs for 6-48 h, and incubated in DMEM medium supplemented with 12% fetal calf serum (FCS), 2 mM L -glutamine, and 100 U/mL penicillin and streptomycin at 37°C and 5% CO₂. The cells were washed with PBS twice, centrifuged at 1500 rpm for 5 min, and 5-10⁵ cells were collected. Binding buffer suspension (500 μL) was added to the cells, and then 5 μL of the FITC-Annexin V mix was added. Next, 5 μL of the PI mix was added, and the suspension was mixed and kept at room temperature for 30 min in the dark. Analysis was with a BD accur C6 flow cytometer.