Antibodies used were as follows: Notch-1 (4380T, Cell Signaling), DLL-1 (ab85346, Abcam), RBPjk (ab180588, Abcam), TAL-1 (PA5-30586, Invitrogen), GAPDH (GTX100118, GeneTex), DENV envelope monoclonal antibody D1-4G2-4-15 (HB-112, ATCC). HRP-conjugated secondary antibodies were purchased from Jackson Immunochemicals. Fluorescence-conjugated Alexa fluor 488 and 647, and Annexin V-alexa 488 were from Invitrogen. FACS antibodies CD61-APC and CD42a-Alexa 488 were from BD Biosciences and CD41-PE from Biolegend.
Cd61 apc
Manufactured by BD
Sourced in Germany
The CD61-APC is a laboratory instrument used for the detection and analysis of CD61 surface antigen expression on cells. It utilizes the Allophycocyanin (APC) fluorochrome to label the CD61 antibody, enabling the identification and quantification of CD61-positive cells through flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Cd41 pe, by BioLegend (1 mentions)
Gapdh gtx100118, by GeneTex (1 mentions)
Alexa fluor 488 and 647, by Thermo Fisher Scientific (1 mentions)
Annexin 5 alexa 488, by Thermo Fisher Scientific (1 mentions)
Cd38 fitc, by BD (1 mentions)
Bcl 2 pe, by BD (1 mentions)
Fix perm kit, by BD (1 mentions)
Bax fitc, by BD (1 mentions)
Facsdiva version 5, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Annexin 5 fitc, by BD (1 mentions)
Cd62p pe, by BD (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
3 protocols using cd61 apc
1
Notch Signaling Pathway Antibodies
2
Multiparametric Flow Cytometry Analysis
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The expanded cells were subjected to phenotypic characterization using a panel of anti-bodies. For the detection of intracellular proteins the cells were permeabilized using a BD fixperm kit (BD Pharmingen, San Jose, CA, USA) and the staining was done according to the manufacturer’s instructions. Isotype matched antibodies were used as controls. The fluorescently labeled cells were acquired on FACS Canto II and Aria (BD, San Jose, CA, USA) and data were analyzed by FACS DIVA - version 5.0 The details of the antibodies used are as follows: annexin V FITC, CD34 APC/PE, CD38 FITC, Bcl-2 PE, Bax FITC, CD33 FITC/PE, CD19 APC CD3 FITC, CD61 APC, CD45 PE/PE-CY-7 murine CD45.1 Pacific blue (BD Pharmingen) and CD133 PE (Milteny Biotech, Colonge, Germany).
3
Platelet Activation Analysis by Flow
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Small aliquots were used for quality control of platelet activation by flow cytometry. Samples were stained with CD61-APC (BD Biosciences, 1:400) and CD62P-PE (BD Biosciences, 1:100) or with secondary anti-Alpaca IgG-AF488 (Jackson ImmunoResearch, 1:400) for 15 minutes at RT, diluted in assay buffer, and immediately read on a FACS Canto II flow cytometer (BD Biosciences). In some cases, fixed platelets were permeabilized with 0.05% saponin before staining. Forward scatter (FSC) and side scatter (SSC) parameters were used to gate platelets and single cells, whereas single stains and isotype controls were used to determine fluorescence gating.
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