The sequences of TP1–TP10 were analyzed using an Applied Biosystems 494 protein sequencer (Perkin Elmer, USA) (22 (link)). Edman degradation was performed according to the standard program supplied by Applied Biosystems (Shimazu, Kyoto, Japan). The MWs of TP1–TP10 were determined by using a Q-TOF mass spectrometric device combined with an ESI source (47 (link)). Nitrogen was maintained at 40 psi for nebulization and 9 L/min at 350°C for evaporation temperature. The data were collected in the centroid mode from m/z 200 to 2,000.
494 protein sequencer
Manufactured by PerkinElmer
Sourced in United States
The 494 protein sequencer is a laboratory instrument designed for the analysis and identification of protein samples. It performs automated Edman degradation, a widely used technique for determining the amino acid sequence of proteins. The 494 sequencer provides accurate and reliable protein sequencing data to support various scientific and research applications.
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Lab products found in correlation
4 protocols using 494 protein sequencer
1
Protein Sequence and Mass Analysis
2
Protein Sequencing and Mass Spectrometry Analysis
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The sequences of STAP1 to STAP14 were analyzed using an Applied Biosystems 494 protein sequencer (Perkin Elmer, USA) (9 (link)). The MWs of STAP1 to STAP14 were determined by employing a quadrupole time-of-flight (Q-TOF) mass spectrometric device (Micromass, Waters, USA) in the combination of an electrospray ionization (ESI) source (50 (link)).
3
Protein Sequence Analysis of TRP Peptides
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The sequences of TRP3, TRP6, TRP9, and TRP12 were analyzed by employing an Applied Biosystems 494 protein sequencer (Perkin Elmer, United States) (10 (link)). The MWs of TRP3, TRP6, TRP9, and TRP12 were detected by an ESI-Q-TOF-MS (Micromass, Waters, United States) (49 (link)).
4
Protein Sequence and Mass Analysis
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The sequences of TMAP1 to TMAP6 were analyzed using an Applied Biosystems 494 protein sequencer (Perkin Elmer, USA) [58 (link)]. Edman degradation was performed according to the standard program supplied by Applied Biosystems.
The precise MWs of TMAP1 to TMAP6 were determined by employing a Q-TOF mass spectrometric device (Micromass, Waters, Milford, MA, USA) in the combination of an electrospray ionization (ESI) source [64 (link)]. Nitrogen was maintained at 40 psi for nebulization and 9 L/min at 350 °C for evaporation temperature. The data were collected in centroid mode from m/z 200 to 2000.
The precise MWs of TMAP1 to TMAP6 were determined by employing a Q-TOF mass spectrometric device (Micromass, Waters, Milford, MA, USA) in the combination of an electrospray ionization (ESI) source [64 (link)]. Nitrogen was maintained at 40 psi for nebulization and 9 L/min at 350 °C for evaporation temperature. The data were collected in centroid mode from m/z 200 to 2000.
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