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Det01cfc

Manufactured by Thorlabs
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The DET01CFC is a silicon photodetector from Thorlabs. It has an active area of 1 mm² and operates in the wavelength range of 350 nm to 1100 nm.

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Lab products found in correlation

Timeharp200, by PicoQuant (2 mentions) Pump 11 pico plus elite, by Harvard Apparatus (1 mentions) Mso64, by Tektronix (1 mentions)

5 protocols using det01cfc

1

Interferometric Deformability Cytometry Protocol

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Operation of interferometric deformability cytometry devices is detailed in previous publications12 (link), 14 (link). Cells injected by a syringe pump (Harvard Apparatus, Pump 11 Pico Plus Elite) shift the optical resonances of the Fabry–Perot interferometer towards longer wavelengths when flowing through its cavity. A tunable laser source (Agilent, 8164B) locked the emitted wavelength to match the resonance peak in absence of cells. Specific wavelengths used for all reported data of this paper range between 1550 and 1600 nm. A fast InGaAs infrared photodetector (Thorlabs, DET01CFC) recorded the optical power variations in time and relayed it to a high-speed digitizer (National Instruments, NI-PXI 5114). A custom MatLab algorithm as well as FlowJo were used to perform curve computation and population analysis respectively. A sensitivity of approximately 950 nm/RIU was previously measured using certified refractive index oils, thus corresponding to a limit of detection of 1.6×10-5RIU when considering an experimentally measured accuracy of 3σ=0.015nm 12 (link). Debris and dead cells were excluded based on their smaller maximum and cell time values whereas doublets and artefacts were excluded based on the larger curve width.
Leblanc-Hotte A., Audiger C., Chabot-Roy G., Lombard-Vadnais F., Delisle J.S., Peter Y.A, & Lesage S. (2023). Immature and mature bone marrow-derived dendritic cells exhibit distinct intracellular mechanical properties. Scientific Reports, 13, 1967.
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2

Laser-Driven SiN Ring Resonator Coupling

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The InP/Si/SiN laser is mounted on a temperature-controlled ceramic stage, and the probe pads are wire bonded in a butterfly-type package. The whole package can be positioned on an XYZ and rotation stage for butt coupling to a SiN high-Q ring resonator chip which is mounted on a separate stage. The laser injection current is tuned to match the laser wavelength to a ring resonance. Self-injection locking is confirmed from the output power on a photodiode (Thorlabs DET01CFC, a large dip in the output power when the locking state is achieved), together with a fiber unbalanced-MZI based wavemeter for monitoring laser frequency stability, where a locked state shows quiet output power trace in the time-domain after the wavemeter on a real-time oscilloscope (Tektronix MSO64).
Xiang C., Guo J., Jin W., Wu L., Peters J., Xie W., Chang L., Shen B., Wang H., Yang Q.F., Kinghorn D., Paniccia M., Vahala K.J., Morton P.A, & Bowers J.E. (2021). High-performance lasers for fully integrated silicon nitride photonics. Nature Communications, 12, 6650.
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3

Ultrafast Optical Spectrum Analysis

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An optical spectrum analyzer (OSA, Yokogawa AQ6370C-20) with a resolution of 0.02 nm and a 1 GHz real-time oscilloscope (OSC, Agilent DSO9104A) together with a 2 GHz photodetector (PD, Thorlabs DET01CFC) are respectively employed to monitor the optical spectra and the pulse train of DSs. RF spectrum is analyzed by a 3 GHz electrical spectrum analyzer (ESA, Agilent N9320B). Finally, the pulse profile is measured by a commercial autocorrelator (Femtochrome FR-103HS).
Luo Y., Cheng J., Liu B., Sun Q., Li L., Fu S., Tang D., Zhao L, & Liu D. (2017). Group-velocity-locked vector soliton molecules in fiber lasers. Scientific Reports, 7, 2369.
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4

Fluorescence Lifetime Measurement Protocol

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For fluorescence lifetime measurements, a pulse picker (Model 350-160, ConOptics) was placed in the laser beam to reduce the pulse frequency from 80 MHz to 20 MHz. Samples (2 μM dye diluted in 50 mM HEPES, pH 7.2, H2O, or CH3OH) were excited at 830 nm laser wavelength and 6 mW laser power. The emitted light was collected by the fast-timing APD and fed to the single-photon counting board (TimeHarp200; PicoQuant). Timing pulses were obtained from a PIN diode (DET01CFC; ThorLabs) monitoring the 20 MHz pulse train. The temporal impulse response of the system was determined by second harmonic generation of laser pulses using a thin nonlinear crystal in place of a dye sample. The lifetime decay data was fit to a single exponential decay function using a custom MATLAB program. Lifetime value of the reference fluorescein dye measured using our system was 4.025 ± 0.015 ns (R2 = 0.99) compared to a literature value of 4.1 ± 0.1 ns.45
Grimm J.B., English B.P., Chen J., Slaughter J.P., Zhang Z., Revyakin A., Patel R., Macklin J.J., Normanno D., Singer R.H., Lionnet T, & Lavis L.D. (2015). A general method to improve fluorophores for live-cell and single-molecule microscopy. Nature methods, 12(3), 244-250.
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5

Fluorescence Lifetime Measurement Protocol

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For fluorescence lifetime measurements, a pulse picker (Model 350-160, ConOptics) was placed in the laser beam to reduce the pulse frequency from 80 MHz to 20 MHz. Samples (2 μM dye diluted in 50 mM HEPES, pH 7.2, H2O, or CH3OH) were excited at 830 nm laser wavelength and 6 mW laser power. The emitted light was collected by the fast-timing APD and fed to the single-photon counting board (TimeHarp200; PicoQuant). Timing pulses were obtained from a PIN diode (DET01CFC; ThorLabs) monitoring the 20 MHz pulse train. The temporal impulse response of the system was determined by second harmonic generation of laser pulses using a thin nonlinear crystal in place of a dye sample. The lifetime decay data was fit to a single exponential decay function using a custom MATLAB program. Lifetime value of the reference fluorescein dye measured using our system was 4.025 ± 0.015 ns (R2 = 0.99) compared to a literature value of 4.1 ± 0.1 ns.45
Grimm J.B., English B.P., Chen J., Slaughter J.P., Zhang Z., Revyakin A., Patel R., Macklin J.J., Normanno D., Singer R.H., Lionnet T, & Lavis L.D. (2015). A general method to improve fluorophores for live-cell and single-molecule microscopy. Nature methods, 12(3), 244-250.
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