Novoscript plus all in one supermix kit

Total RNA was extracted from sweet oranges using the TRIzol reagent (Ambion, Waltham, MA, USA) and purified using the EasyPure Plant RNA Kit (TianGen, Beijing, China). First-strand cDNA synthesis was performed using the NovoScript Plus All-in-One SuperMix kit (Novoprotein, Shanghai, China). The specific primers used for qRT-PCR are listed in Supplementary Table S4. Samples of the stems, leaves, and fruits were collected at the same time intervals [40 (link)]. RT-qPCR was performed on a CFX 96 qPCR instrument (Bio-Rad, Hercules, CA, USA) using a TransStart Tip Green qPCR SuperMix kit (TransGen, Beijing, China). The amplification of CsCOX was used as a reference control.

Total RNA of B. distachyon was extracted using TRIzol Reagent (Ambion, Waltham, MA, USA), and purified with an EasyPure Plant RNA Kit (TianGen, Beijing, China). Subsequently, cDNA first-strand synthesis was conducted by reverse transcription using a NovoScript Plus All-in-one SuperMix kit (Novoprotein, Shanghai, China). Specific primers for RT-PCR and qRT-PCR are listed in Supplementary Table S8. The roots, stems, leaves, and seeds were sampled at the same time intervals. To investigate the expression of BdGATA genes in different tissues/organs, we carried out the method for tissue sample collection as previously described [46 (link)]. RT-PCR was conducted using a MonScript RTase II kit (Monad, Suzhou, China). PCR was performed with 10–15 pmol of each primer, DNA templates (60 ng), double-distilled H₂O, and Hieff PCR Master Mix (Yeasen, Shanghai, China) in a total volume of 20 μL. qRT-PCR was performed using a TransStart Tip Green qPCR SuperMix kit (TransGen, Beijing, China) in a CFX 96 qPCR instrument (BioRad, Hercules, CA, USA). BdUBC (Bradi4g00660) was amplified as the normalization control.