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Model 343

Manufactured by PerkinElmer
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The Model 343 is a versatile laboratory instrument designed for a range of analytical applications. It features a robust construction and advanced technology to deliver reliable performance. The core function of the Model 343 is to provide accurate and consistent measurements for your laboratory needs.

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Lab products found in correlation

Avance 3 600 spectrometer, by Bruker (2 mentions) Precoated silica gel gf254 plates, by Qingdao Marine Chemical (2 mentions) Rp 18 silica gel, by Merck Group (2 mentions) Lc 8a, by Shimadzu (2 mentions) Ft ir 480 spectrophotometer, by Jasco (2 mentions) Ez purify 3 system, by Merck Group (2 mentions) Prc ods k column, by Agilent Technologies (2 mentions) Advance 3 spectrometer, by Bruker (1 mentions) 6850 chromatograph, by Agilent Technologies (1 mentions) Silica gel, by Qingdao Marine Chemical (1 mentions) Avance 500 spectrometer, by Bruker (1 mentions) Zorbax sb c 18 75, by Agilent Technologies (1 mentions) Mci gel, by Mitsubishi (1 mentions) Avance 500, by Bruker (1 mentions) J 815 spectrometer, by Jasco (1 mentions) Impact 2, by Bruker (1 mentions) Shim pack xr ods 3, by Shimadzu (1 mentions) Nexera x2, by Shimadzu (1 mentions)

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Model 343 polarimeter (7 citations)

7 protocols using model 343

1

Spectroscopic Analysis of TAMCSAs

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NMR spectra were recorded on a Bruker Advance III spectrometer at 400 MHz for 1H NMR and 100 MHz for C NMR. NOESY experiments of TAMCSAs were completed under a number of scans (1a, 8; 1b–1d, 16), mixing time (1a–1d 0.30 s), and d1 (1a, 1.98 s; 1b, 1.82 s; 1c, 1.99 s; 1d, 1.90 s). HRMS spectra were acquired on MicrOTOF-Q II 10260, FLEX-PC, and TripleTOF 5600units. IR spectra were obtained on a Nicolet 360 Avatar IR spectrometer as KBr pellets. Optical rotations were measured with a PerkinElmer Model 343 polarimeter using the sodium D line at 589 nm. X-ray crystal structures were determined by a Smart Apex II Single Crystal diffractometer.
Feng L., Gao G., Zhao H., Zheng L., Wang Y., Stavropoulos P., Ai L, & Zhang J. (2018). Synthesis of Tripeptide Derivatives with Three Stereogenic Centers and Chiral Recognition Probed by Tetraaza Macrocyclic Chiral Solvating Agents Derived from d-Phenylalanine and (1S,2S)-(+)-1,2-Diaminocyclohexane via 1H NMR Spectroscopy. The Journal of organic chemistry, 83(22), 13874-13887.
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2

Monosaccharide and Fatty Acid Analysis of Capsular Polysaccharides

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Monosaccharides were analyzed as the alditol acetates obtained by hydrolysis of the CPS-1 (2 M CF3COOH, 120 °C, 2 h) and CPS-2 (0.5 M CF3COOH, 100 °C, 3 h) by GC on an Agilent 6850 chromatograph (Santa Clara, CA, USA) equipped with an HP-5 MS capillary column using a temperature program from 150 °C (3 min) to 230 °C (10 min) at 3 °C min−1. The absolute configurations of monosaccharides were determined by GC of the acetylated (S)-2-octyl glycosides as described [40 (link)]. The absolute configuration of d-Xlu was determined by calculation of the specific optical rotation of OS using the Klyne rule [12 (link)]. The specific optical rotation of OS was measured on a PerkinElmer instrument, model 343 (Waltham, MA, USA). Fatty acid analysis was performed by GC of methyl derivatives after methanolysis of CPS with 2 M acetylchloride in methanol (120 °C, 4 h). Proteins were analyzed by the conventional method [41 (link)].
Kokoulin M.S., Romanenko L.A., Kuzmich A.S, & Chernikov O. (2021). Structure of the Cell-Wall-Associated Polysaccharides from the Deep-Sea Marine Bacterium Devosia submarina KMM 9415T. Marine Drugs, 19(12), 665.
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3

Spectroscopic Characterization of Compounds

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Optical rotations were measured with Perkin Elmer/Model-343 digital polarimeter. IR spectra were recorded on a JASCO FT/IR-480 spectrophotometer and reported as wave number (cm−1). 1H, 13C NMR spectra and 2D NMR spectra were recorded on a Bruker Avance III 600 spectrometer. Chemical shifts were reported using TMS as the internal standard. HRESIMS data were obtained on a Bruker Apex IV FTMS spectrometer. Column chromatography (CC) was performed on silica gel (90 − 200 µm; Qingdao Marine Chemical Co. Ltd., Qingdao, People’s Republic of China), and MPLC was performed on a Lisui EZ Purify III System packed with RP-18 silica gel (40–63 μm, Merck, 71 Darmstadt, Germany) columns. Precoated silica gel GF254 plates (Qingdao Marine Chemical Co. Ltd., Qingdao, People’s Republic of China) were used for thin-layer chromatography (TLC). Preparative HPLC was performed on Shimadzu LC-8A equipped with a Shimadzu PRC-ODS(K) column and Agilent 1100 apparatus equipped with a Zorbax SB-C-1875 (Agilent, 9.4 mm × 25 cm) column, respectively. The reagents were purchased from Fisher Scientific at the highest quality and were used without further purification.
Cheng J.T., Guo C., Cui W.J., Zhang Q., Wang S.H., Zhao Q.H., Liu D.W., Zhang J., Chen S., Chen C., Liu Y., Pan Z.H, & Liu A. (2020). Isolation of two rare N-glycosides from Ginkgo biloba and their anti-inflammatory activities. Scientific Reports, 10, 5994.
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4

Optical Rotation of Isopulegol Enantiomers

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EXAMPLE 1

Rotation value measurements were performed for the system L- and D-isopulegol. A Perkin Elmer model 343 polarimeter with a cuvette length of 100 mm and a light source having a wavelength of 589 nm was utilized. All measurements took place at a constant temperature of 25° C. First, the enantiomer ratio of L- and D-isopulegol in % EE was determined by gas chromatography. Table 1 reports the measured values for the rotation values as a function of the EE (excess of L-isopulegol).

TABLE 1
Measured values for the rotation value measurements for
various enantiomer ratios of L- and D-isopulegol.
% EE valueOptical rotation [°]
12.56−0.82
30.84−4.522
58.8−10.42
90.6−17.77
97.88−19.66
99.84−20.17

FIG. 3 shows a graphical plot of the measured values from table 1. The measured values fit a 2nd order polynomial regression very well.

US10669222B2. Process for enriching enantiomers from an enantiomer mixture (2020-06-02). BASF SE [DE]. Inventors: Sebastian Wloch [DE], Gunnar Heydrich [DE], Matthias Rauls [DE].
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5

Comprehensive Structure Elucidation of Compound

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NMR spectra were obtained on a Bruker Avance 500 spectrometer at 25 °C (operating at 500 MHz for 1H-NMR, 125 MHz for 13C-NMR) with residual solvent peaks as references (CDCl3-d6: δH 7.26, δC 77.16). High-resolution electrospray ionization mass spectrometry (HR-ESIMS) measurements were obtained on an Accurate-Mass-Q-TOF LC/MS 6520 instrument (Santa Clara, CA, USA) in the positive ion mode. Optical rotations were measured on a Perkin-Elmer Model 343 polarimeter. Compound structures were elucidated by analyzing 1H NMR, 13C NMR, Heteronuclear Multiple Bond Correlation (HMBC), Heteronuclear Single Quantum Correlation (HSQC), 1H-1H Correlation Spectroscopy (COSY), and comparison with the previously reported data. The structure formula was finally confirmed by HR-ESIMS.
Song F., Zhang K., Yang J., Wilson A.S., Chen C, & Xu X. (2023). The Hypolipidemic Characteristics of a Methanol Extract of Fermented Green Tea and Spore of Eurotium cristatum SXHBTBU1934 in Golden Hamsters. Nutrients, 15(6), 1329.
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6

Analytical Characterization of Organic Compounds

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Optical rotations were measured with Perkin Elmer/Model-343 digital polarimeter. IR spectra were recorded on a JASCO FT/IR-480 spectrophotometer and reported as wave number (cm−1). 1H, 13C NMR spectra and 2D NMR spectra were recorded on a Bruker Avance III 600 spectrometer. Chemical shifts were reported using TMS as the internal standard. HR-ESI–MS data were obtained on a Bruker Apex IV FT-MS spectrometer. Column chromatography (CC) was carried out using D-101 macroreticular resin (Tianjin Polymer Technology Co. Ltd.), MCI gel (75–150 μm, Mitsubishi Chemical Industries, Japan) and silica gel (90–200 µm; Qingdao Marine Chemical Co. Ltd., Qingdao, People’s Republic of China). MPLC was performed on a Lisui EZ Purify III System packed with RP-18 silica gel (40–63 μm, Merck, 71 Darmstadt, Germany) columns. Precoated silica gel GF254 plates (Qingdao Marine Chemical Co. Ltd, Qingdao, People’s Republic of China) were used for thin-layer chromatography (TLC). Preparative HPLC was performed on Shimadzu LC-8A equipped with a Shimadzu PRC-ODS(K) column and Agilent 1100 apparatus equipped with a Zorbax SB-C-1875 (Agilent, 9.4 mm × 25 cm) column, respectively.
Cheng J.T., Chen S.T., Guo C., Jiao M.J., Cui W.J., Wang S.H., Deng Z., Chen C., Chen S., Zhang J, & Liu A. (2017). Triterpenoid Saponins from the Seeds of Aesculus chinensis and Their Cytotoxicities. Natural Products and Bioprospecting, 8(1), 47-56.
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7

Spectroscopic Analysis of Natural Compounds

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Optical rotations were measured at 24 • C on a PerkinElmer Model 343 polarimeter. 1D and 2D NMR spectra were recorded in CD 3 OD, DMSO-d 6 and CDCl 3 in a Bruker 500 MHz NMR instrument (Avance 500). High-resolution mass spectra were obtained in a QTOF, Bruker Daltonics, model Impact II spectrometer in electrospray ionization. C18 columns (75 × 2.0 mm i.d.; 1.6 μm Shim-pack XR-ODS III) were used for UHPLC separation using a Shimadzu, model Nexera X2. Silica gel 60 (0.063-0.200 mm) and silica gel 60 (0.04-0.063 mm) were used for purification of the compounds. TLC was performed on normal phase precoated silica gel 60 G or 60 GF 254 (Merck) plates. The UV and ECD spectra of 1-3 were recorded with a Jasco J-815 spectrometer (Jasco, Tokyo, Japan) in the 195-400 nm region using the following parameters: bandwidth 1 nm; 25 response 1 s; scanning speed 100 nm min -1 ; 3 accumulations; room temperature; sample in methanol solution; 0.1 cm cell path length; concentration 0.2 mg mL -1 .
Pereira Cabral M.R., Cecchetto M., Batista JM J.r., Batista A.N.L., Foglio M.A., Tasca Gois Ruiz A.L., Barrotto do Carmo M.R., Ferreira da Costa W., Baldoqui D.C, & Sarragiotto M.H. (2020). Cytotoxic sesquiterpene lactones from Campuloclinium macrocephalum (=Eupatorium macrocephalum). Phytochemistry, 179.
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