Lichro cart superspher si 60 column

Quantification of α-tocopherol was evaluated according to AOCS Ce 8-89 (1993) [35 ] as described by Fuentes et al. [39 (link)] using an HPLC consisting of a Merck-Hitachi pump L-6200A (Merck, Darmstadt, Germany), a Rheodyne 7725i injector with 20 μL sample loop, a LiChro-CART Superspher Si 60 column (25 cm × 4 mm id, 5 μm particle size; Merck, Darmstadt, Germany), a Hitachi Chromaster 5440 fluorescence detector, and a PC with Clarity chromatographic software (DataApex, Prague, The Czech Republic) to process the chromatographic signal detected at 290 nm and 330 nm, and the excitation and emission wavelengths, respectively. The alpha tocopherol standard from Calbiochem (Merck, Darmstadt, Germany) was used. The results were expressed in mg/kg.

Tocopherol contents of the oil obtained by extraction with sc-CO₂, were determined using a High Performance Liquid Chromatography (HPLC) system according to the standard method AOCS Ce 8-89 [27 ] LiChro-CART Superspher Si 60 column (ID 250 × 4 mm, particle size 5 µm; Merck, Darmstadt, Germany) was used. The mobile phase was propan-2-ol in hexane (1.0/99.0, v/v) at a flow rate of 1 mL/min. The HPLC system consisted of a Merck-Hitachi L-6200A pump (Merck, Darmstadt, Germany), a Rheodyne 7725i injector with a 20 μL sample loop, a Hitachi Chromaster 5440 fluorescence detector, and a Merck-Hitachi D-chromato-integrator 2500. The excitation and emission wavelengths were in the ranges of 290 nm and 330 nm, respectively. Tocopherols were identified using Calbiochem standards (Merck, Darmstadt, Germany) as external standards.