Human colon cancer cell lines, RKO and HCT-8, were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA). RKO and HCT-8 cells were cultured in DMEM supplemented with 10% fetal bovine serum (Corning, USA) and 1% penicillin/streptomycin solution (Invitrogen). Both cell lines were cultured at 37˚C under a humidified atmosphere of 5% CO2.
Hct 8
Manufactured by Corning
Sourced in United States
The HCT-8 is a lab equipment product designed for general laboratory use. It functions as a high-capacity temperature-controlled centrifuge capable of processing multiple samples simultaneously. The HCT-8 provides consistent, reliable performance for a variety of applications requiring temperature-controlled centrifugation.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Corning (2 mentions)
Mcf 7, by Corning (2 mentions)
Dulbecco modified eagle medium (dmem), by Corning (2 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
A2780, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hct 8, by Keygen Biotech (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Mcf 7, by Cell Resource Center (1 mentions)
A2780, by Keygen Biotech (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Hcc1806, by Corning (1 mentions)
Kyse150, by Corning (1 mentions)
Hcclm3, by National Collection of Authenticated Cell Cultures (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Hct 8, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Kyse150, by National Collection of Authenticated Cell Cultures (1 mentions)
4 protocols using hct 8
1
Culturing Human Colon Cancer Cells
2
Establishment and Characterization of Cancer Cell Lines
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All cell lines used were of human origin. A549 (non-small cell lung cancer) and MCF-7 (breast cancer) cell lines were purchased from Cell Resource Center of Shanghai Institute for Biological Sciences (Chinese Academy of Sciences, Shanghai, China). A549T (Taxol-resistant A549 subline) and MCF-7R (Adriamycin-resistant MCF-7 subline) cell lines were from Shanghai Aiyan Biological Technology Co. Ltd (Shanghai, China). HCT-8 (colon carcinoma), A2780 (ovarian cancer), HCT-8T (Taxol-resistant HCT-8 subline) and A2780T (Taxol-resistant A2780 subline) were from Nanjing KeyGEN BioTECH Co. Ltd (Nanjing, China). PC-3 (prostate cancer) was from Typical Culture Preservation Commission Cell Bank, Chinese Academy of Sciences (Shanghai, China).
All cell lines were routinely cultured at 37 °C in humidified atmosphere with 5% CO2. Unless stated otherwise, the growth medium used for A549, A549T, MCF-7R, HCT-8, HCT-8T, PC-3 and A2780T cells was RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Corning). MCF-7 and A2780 cells were propagated in Dulbecco’s Modified Eagle Medium (DMEM, Corning) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Corning). For comparison, some experiments replaced FBS with other serums (calf serum, bovine serum, goat serum, horse serum or human serum) and the same concentration.
All cell lines were routinely cultured at 37 °C in humidified atmosphere with 5% CO2. Unless stated otherwise, the growth medium used for A549, A549T, MCF-7R, HCT-8, HCT-8T, PC-3 and A2780T cells was RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Corning). MCF-7 and A2780 cells were propagated in Dulbecco’s Modified Eagle Medium (DMEM, Corning) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Corning). For comparison, some experiments replaced FBS with other serums (calf serum, bovine serum, goat serum, horse serum or human serum) and the same concentration.
3
Cell Culture and Delipidized Medium
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MDA-MB-231, HeLa, HCT116, HCT-8, HCC1806, SKHEP-1, HAEC, HPAC, HepG2, Huh7, and 293T were obtained from the American Tissue Culture Collection. HCCLM3, L02, and KYSE150 were obtained from China Center for Type Culture Collection. HCT-8, HCC1806, and KYSE150 cells were cultured in 1640 (Corning), and other cells were cultured in DMEM (Corning). The above mediums were supplemented with 10% FBS (#900-108; Gemini) and 100 U/ml penicillin and streptomycin (#15140122; Gibco). All cells were cultured in a humidified atmosphere of 5% CO2 at 37°C.
For delipidized medium, cells were first seeded in the medium containing regular 10% FBS and were switched into the medium containing 10% delipidized FBS (#P30-3402; PAN) upon treatment on the following day.
For delipidized medium, cells were first seeded in the medium containing regular 10% FBS and were switched into the medium containing 10% delipidized FBS (#P30-3402; PAN) upon treatment on the following day.
4
Cell Culture Conditions for Diverse Cancer Lines
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HCT8, AGS, HePG2, MCF-7, and PC9 cell lines were obtained from the American Type Culture Collection (ATCC). HCT8 and PC9 cells were cultured in Roswell Park Memorial Institute-1640 (RPMI1640, Corning, USA) with 10% FBS and 1% Penicillin and Streptomycin at 37 °C in 5% CO2. AGS, HePG2, and MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Corning, USA), with 10% FBS and 1% Penicillin and Streptomycin at 37 °C in 5% CO2. All cell lines were stored in multiple backups upon receipt to reduce risk of phenotypic drift, and tested to determine whether they were mycoplasma-free by Mycoplasma Stain Assay Kit.
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