Mouse anti chicken cd4 alexa fluor 647 clone ct 4

Mouse anti-chicken CD4-Alexa Fluor® 647 (clone CT-4, Cat. no. 821031), mouse anti-chicken CD8α-PE (clone 3-298, Cat. no. 8405–09), mouse anti-chicken CD3-SPRD (clone CT-3, Cat. no. 8200-13), and mouse anti-chicken Bu-1-FITC (clone AV20, Cat. no. 8395-02) antibodies were purchased from SouthernBiotech (Birmingham, AL, USA). The mouse anti-chicken monocyte/macrophage-PE antibody (clone 5K102, also called clone KUL01, Cat. no. M4520-17) was purchased from USBiological (Salem, MA, USA). Mouse anti-chicken MHC class II-FITC (clone 2G11, Cat. no. ab24882), mouse anti-chicken MHC class I-FITC (clone F21-2, Cat. no. ab24881), and biotin mouse anti-chicken TCR gamma delta (clone TCR1, Cat. no. ab25151) antibodies were purchased from Abcam. Streptavidin Alexa Fluor 750 conjugate (Cat. no. S21384) and Sytox blue dead cell stain (Cat. no. S34857) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The viability determination reagent 7-AAD (Cat. no. 8200-02), was purchased from BD Biosciences (San Jose, CA, USA).

Mouse Anti-Chicken CD4-Alexa Fluor® 647 (clone CT-4, Catalog # 8210–31), Mouse Anti-Chicken CD8α PE (clone 3–298, Catalog # 8405–09) were purchased from SouthernBiotech company (AL, USA). The viability determination reagent, 7AAD, was purchased from BD Biosciences (CA, USA, Catalog # 559925).