B6 cg ndor1tg ubc cre ert2 1ejb 1j

The care and use of animals for all experiments described conformed to NIH guidelines. Experimental mice were housed with a 12:12 hr light:dark cycle with free access to chow and water, in standard laboratory cages located in a temperature and humidity-controlled vivarium. The protocols for care and use of animals were approved by the Institutional Animal Care and Use Committees at the University of Virginia, which is accredited by the American Association for the Accreditation of Laboratory Animal Care. C57BL/6J (Bl6, from Jackson Laboratory, ME, USA) and CBA/J (from Jackson Laboratory, ME, USA) mice and sibling mice served as control mice for this study. Neonatal mouse pups (postnatal day 0 [P0]–P5) were sacrificed by rapid decapitation, and mature mice were euthanized by CO₂ asphyxiation followed by cervical dislocation. The FLEx-β-actin-EGFP (C57BL/6-Tg(CAG-tdTomato,Actb/EGFP)1Erv/J, Jackson Laboratories strain #029889) mice were purchased from The Jackson Laboratory. The Ubc-Cre^ERT2 mice (B6.Cg-Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/1J, Jackson Laboratories strain #007001) were gifted from Dr. Alban Gaultier (University of Virginia). All procedures involving Elmod1 knockout animals met the guidelines described in the National Institutes of Health Guide for the Care and Use of Laboratory Animals and was approved by the Animal Care and Use Committees of Tel Aviv University (M-12-046).

C;129S-Vhl^tm1Jae/J (stock no. 4081, Jackson Laboratories, Bar Harbor, ME, USA) were used to generate the UBC-Cre-ER^T2 Vhl^LoxP/LoxP mice. These mice harbor two loxP sites flanking the promoter and exon 1 of the murine Vhl locus [63 (link)]. The C;129S-Vhl^tm1Jae/J mice were crossed with B6.Cg-Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/1J or UBC-Cre-ER^T2 mice (Jackson Laboratories, stock no. 008085) that ubiquitously express a tamoxifen-inducible Cre recombinase (Cre-ER^T2) [64 (link)]. UBC-Cre-ER^T2 Vhl^LoxP/LoxP mice were generated through the appropriate crosses, along with the corresponding control mice. Then UBC-Cre-ER^T2 Vhl^LoxP/LoxPHif1a^LoxP/LoxP mice were generated using B6.129-Hif1a^tm3Rsjo/J mice (Jackson Laboratories, stock no. 007561) that harbor two loxP sites flanking exon 2 of the murine Hif1a locus [65 (link)]. These mice were then crossed with B6.Cg-Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/1J mice as described above to generate UBC-Cre-ER^T2 Hif1a^LoxP/LoxP mice, which were subsequently crossed with C;129S-Vhl^tm1Jae/J mice to generate UBC-Cre-ER^T2 Vhl^LoxP/LoxPHif1a^LoxP/LoxP mice and their corresponding control mice. The UBC-Cre-ER^T2 Vhl^LoxP/LoxPHif2a^LoxP/LoxP mice were generated through the appropriate crosses using Epas1^tm1Mcs/J mice (Jackson Laboratories, stock no. 008407) [66 (link)].