Transmission electron microscopy (JEM-F200, Japan) and an accompanying energy spectrometer were used to observe the microstructure and elemental distribution of FePCN.. XPert Pro instrument (The Netherlands) was used to analyze all powder X-ray diffraction (XRD) data. Fourier transform infrared spectrum analyzer (FT-IR5700, USA) was utilized for FT-IR spectroscopy. A UV spectrophotometer (UV-2600, Shimadzu, Japan) was used to obtain UV–visible absorption spectra. A Leica STELLARIS 5 SR (Leica, Germany) was used to acquire confocal laser fluorescence microscopy (CLSM) images. A flow cytometer (FCM, cytoFlex S, Beckman) was used to analyze the FCM data. Cell viability was assessed by a CCK-8 assay with a microplate reader (VICTOR Nivo, PerkinElmer) at 450 nm. In vivo, an animal imaging system (IVIS Lumina XRMS, PerkinElmer, USA) was utilized for fluorescence imaging.
Stellaris 5 sr
Manufactured by Leica
Sourced in Germany
The STELLARIS 5 SR is a confocal microscope system designed for high-resolution imaging of biological samples. It features a supercontinuum white light laser source and a high-speed resonant scanner, enabling rapid acquisition of images with excellent detail and contrast.
Automatically generated - may contain errors
Lab products found in correlation
Victor nivo, by PerkinElmer (1 mentions)
Ivis lumina xrms, by PerkinElmer (1 mentions)
Cytoflex s, by Beckman Coulter (1 mentions)
Uv 2600, by Shimadzu (1 mentions)
Dylight 488 conjugated goat anti mouse igg, by Abbkine (1 mentions)
Cy3 labeled goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions)
2 protocols using stellaris 5 sr
1
Multi-Technique Characterization of FePCN
2
Immunofluorescence Staining of Caco-2 Cells
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Caco-2 cells grown on the confocal dish were fixed with 4% paraformaldehyde for 15 min and permeabilized by exposure to 0.5% Triton X-100 in PBS for 15 min. After blocking with 5% bovine serum albumin (BSA) for 1 h at room temperature, the cells were incubated with primary antibodies at 4 ׄ°C overnight. The primary antibodies used were as follows: YAP Rabbit mAb (Cat#:14074, 1:100, CST), Ezh2 Mouse mAb (Cat#:3147, 1:100, CST). After thorough washing with PBS containing 0.1% Tween20, these cells were incubated with Cy3-labeled goat anti-rabbit IgG (Servicebio, China) and DyLight 488-conjugated goat anti-mouse IgG (Abbkine, China) in the dark for 1 h at room temperature. Nuclei were visualized through DAPI staining. Immunofluorescence images were obtained using a confocal microscope (Leica STELLARIS 5 SR, Germany).
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