Accela 80 hz

The PAC’s mDP and %Gal were determined following the phloroglucinolysis method described by Kennedy et al. [31 (link)] with slight modifications. Briefly, 10 to 100 mg of sample, depending on PAC concentration, were dissolved in 1.0 mL of a freshly prepared methanol solution containing 50 g/L of phloroglucinol, 10 g/L of ascorbic acid, and 0.1 M of hydrochloric acid. Insolubilized material was removed by centrifugation, after which 400 µL of the supernatant were transferred to pressure resistant vials and incubated at 50 °C for 1 h followed by the addition of 2 mL of 40 mM sodium acetate aqueous solution to stop the reaction. Depolymerization products were quantified by high performance liquid chromatography (HPLC) in a Accela 80 Hz (Thermo Fisher Scientific, San Jose, CA, USA) (Figure S1 in Supplementary Materials) and peak attribution was made by electrospray ionisation mass spectrometry (ESI-MS) in a LCQ Fleet ion trap mass spectrometer (ThermoFinnigan, San Jose, CA, USA) (Table S1 in Supplementary Materials) operated as described elsewhere [32 (link)]. mDP was calculated by dividing the sum of terminal and extension units by the sum of terminal units and the %Gal was calculated by dividing the sum of galloylated units by the sum of all units.