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Cd86 percp vio700

Manufactured by Miltenyi Biotec
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CD86-PerCp-Vio700 is a fluorescently labeled antibody product from Miltenyi Biotec. It is designed for the detection and analysis of CD86 expression on cells by flow cytometry.

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Lab products found in correlation

Hladr pacific blue, by BioLegend (1 mentions) Tlr7 8 ligand r848, by InvivoGen (1 mentions) Gm csf, by Miltenyi Biotec (1 mentions) Interleukin 4 (il 4), by Miltenyi Biotec (1 mentions) Mouse igg1κ, by Miltenyi Biotec (1 mentions) Galectin 9 pe, by Miltenyi Biotec (1 mentions)

2 protocols using cd86 percp vio700

1

Dendritic Cell Activation Assay

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Immature dendritic cells (moDCs) were derived from monocytes cultured in granulocyte–macrophage colony‐stimulating factor (GM‐CSF, 20 ng mL−1) and IL‐4 (10 ng mL−1), both from Miltenyi Biotec, for 7 days. To confirm the dendritic cell phenotype, the expression of anti‐CD1a‐FITC (HI149; BD Pharmingen) was verified. For stimulation assays, moDCs were infected, and the next day, activation was measured in live cells by flow cytometry using the antibodies against CD80‐PE (REA661; Miltenyi Biotec), CD83‐APC (REA714; Miltenyi Biotec), CD86‐PerCp‐Vio700 (FM95; Miltenyi Biotec) and HLA‐/DR Pacific Blue (1243; BioLegend). To control activation, the cells were activated with the TLR7/8 ligand R848 diluted to 10−4 M (InvivoGen).
Ramos R.N., Tosch C., Kotsias F., Claudepierre M., Schmitt D., Remy‐Ziller C., Hoffmann C., Ricordel M., Nourtier V., Farine I., Laruelle L., Hortelano J., Spring‐Giusti C., Sedlik C., Le Tourneau C., Hoffmann C., Silvestre N., Erbs P., Bendjama K., Thioudellet C., Quemeneur E., Piaggio E, & Rittner K. (2022). Pseudocowpox virus, a novel vector to enhance the therapeutic efficacy of antitumor vaccination. Clinical & Translational Immunology, 11(5), e1392.
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2

Immune Checkpoint Ligand Expression Analysis

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Immune checkpoint ligand expression analysis by tumor cells was performed in analogy to the stress ligand expression analysis detailed above, using the following antibodies: anti-human CD80-BV421 (Biolegend, mouse IgG1,κ, clone 2D10, 1:20), CD86-PerCP-Vio 700 (Miltenyi Biotec, Bergisch Gladbach, REA968, 1:50), CD112-APC (Miltenyi Biotec, REA1195, 1:50), CD155-PE-Vio 770 (Miltenyi Biotec, REA1081, 1:50), Galectin-9-PE (Miltenyi Biotec, REA435, 1:50), and CD274 (PD-L1)-Vio Bright B515 (Miltenyi Biotec, REA1197, 1:50). The mouse IgG1,κ antibody-BV421 (Biolegend, clone MOPC-21) and respective REA controls (Miltenyi Biotec, REA293) were used as isotype controls.
Troschke-Meurer S., Zumpe M., Meißner L., Siebert N., Grabarczyk P., Forkel H., Maletzki C., Bekeschus S, & Lode H.N. (2023). Chemotherapeutics Used for High-Risk Neuroblastoma Therapy Improve the Efficacy of Anti-GD2 Antibody Dinutuximab Beta in Preclinical Spheroid Models. Cancers, 15(3), 904.
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