Gemcitabine hcl

Manufactured by Selleck Chemicals

Sourced in United States, China

Gemcitabine HCl is a chemical compound used as a laboratory reagent. It is a hydrochloride salt of the nucleoside analog gemcitabine, which is commonly used in cancer research and development. The product is supplied as a white to off-white crystalline powder.

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Lab products found in correlation

Cisplatin, by Selleck Chemicals (2 mentions) Triciribine, by Merck Group (1 mentions) Mia paca 2, by American Type Culture Collection (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Phospho s6 ser235 236, by Cell Signaling Technology (1 mentions) Phospho erk thr202 tyr204, by Cell Signaling Technology (1 mentions) Panc 1, by American Type Culture Collection (1 mentions) Aspc 1, by American Type Culture Collection (1 mentions) Rapamycin, by Merck Group (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions) Phospho yap ser127, by Cell Signaling Technology (1 mentions) Phospho lats1 ser909, by Cell Signaling Technology (1 mentions) Pd184352, by Merck Group (1 mentions) Doxorubicin hcl, by LC Laboratories (1 mentions) Celltiter 96 aqueous one solution cell proliferation kit, by Promega (1 mentions) Hydroxypropyl methylcellulose, by Merck Group (1 mentions) Osimertinib, by Selleck Chemicals (1 mentions) Carboplatin, by Selleck Chemicals (1 mentions)

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Gemcitabine (138 citations)

6 protocols using gemcitabine hcl

Anticancer Drug Screening in Cell Lines

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MiaPaCa2, MDA-MB231, Panc1, AsPC1, and HEK293T cell lines were obtained from American Type Culture Collection (ATCC) and mycoplasma-free. These cells were cultured in Dulbecco’s minimal essential medium (DMEM) from Nacalai (California, USA) supplemented with 10% v/v FBS and penicillin (100 U/mL)/streptomycin (100 μg/mL) from Hyclone (IL, USA). Antibodies for GAPDH (14C10, #2118), phospho-AKT Ser473 (#9271), phospho-ERK Thr202/Tyr204 (#9101), phospho-S6 Ser235/236 (#2211), YAP (#4912), phospho-YAP Ser127 (#4911), and phospho-LATS1 Ser909 (#9157) were from Cell Signaling Technology (MA, USA). Antibody for TAZ (#HPA007415) was from Sigma-Aldrich (MO, USA). Gemcitabine-HCl (#S1149) was obtained from Selleck Chemicals (TX, USA), while doxorubicin-HCl (#D-4000) from LC Laboratories (MA, USA). PD184352, Triciribine and Rapamycin were obtained from Sigma-Aldrich (MO, USA). The Cell viability was assayed by colorimetric based CellTiter 96^® AQueous One Solution Cell Proliferation kit (#G3581, Promega), per manufacture’s protocol.

Chai T.F., Manu K.A., Casey P.J, & Wang M. (2020). Isoprenylcysteine carboxylmethyltransferase is required for the impact of mutant KRAS on TAZ protein level and cancer cell self-renewal. Oncogene, 39(31), 5373-5389.

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Preparation and Formulation of Anticancer Agents

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For in vitro studies, 6-thio-dG (Metkinen Oy, Kuopio, Finland) was dissolved in DMSO/water (1:1) to prepare 10 mM stock solutions, which were kept frozen at −20°C. A 1 mM, final concentration stock was prepared for in vitro experiments and added in fresh medium at different concentrations. Erlotinib HCl (Selleckchem, Houston, TX) was dissolved in DMSO to prepare 10 mM stock solutions, which were kept frozen at −80°C. A 2.5 μM erlotinib final concentration was used for in vitro experiments. Osimertinib and paclitaxel (Selleckchem) were dissolved in DMSO, and carboplatin (Selleckchem) was dissolved in water to prepare 10 mM stock solutions, which were kept frozen at −80°C. A 1 mM final concentration stock was prepared for in vitro experiments and added in fresh medium at different concentrations.
For mouse in vivo studies, 6-thio-dG was prepared in 5% DMSO for intraperitoneal (i.p.) injection or 0.4% (hydroxypropyl)methyl cellulose (Sigma, Saint Louis, MO) for oral gavage. Erlotinib was prepared in 15% Captisol (β-cyclodextrin, sulfobutyl ethers, sodium salts) (Cydex Pharmaceuticals, Lawrence, KS) for oral gavage. Gemcitabine HCl and cisplatin (Selleckchem) were dissolved in 0.9% NaCl saline solution for i.p. injections.

Mender I., LaRanger R., Luitel K., Peyton M., Girard L., Lai T.P., Batten K., Cornelius C., Dalvi M.P., Ramirez M., Du W., Wu L.F., Altschuler S.J., Brekken R., Martinez E.D., Minna J.D., Wright W.E, & Shay J.W. (2018). Telomerase-Mediated Strategy for Overcoming Non–Small Cell Lung Cancer Targeted Therapy and Chemotherapy Resistance. Neoplasia (New York, N.Y.), 20(8), 826-837.

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AZD1775 and Gemcitabine HCL Storage

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AZD1775 and gemcitabine HCL were purchased from Selleck Chem. The drugs were reconstituted in dimethyl sulfoxide (DMSO) and stored at −20ºC. In each experiment, an equivalent amount of DMSO for the highest drug concentration was used as vehicle/control.

Moreira D.C., Venkataraman S., Subramanian A., Desisto J., Balakrishnan I., Prince E., Pierce A., Greisinger A., Green A., Eberhardt C.G., Foreman N.K, & Vibhakar R. (2020). Targeting MYC-Driven Replication Stress in Medulloblastoma with AZD1775 and Gemcitabine. Journal of neuro-oncology, 147(3), 531-545.

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Brusatol and Gemcitabine Combination Protocol

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Brusatol was purchased from Tauto Biotech (E-1044; Shanghai, China). Gemcitabine HCl for the in vitro experiments was purchased from Shanghai Boyun Biotech Co. Ltd. (BY13174; Shanghai, China), and Gemcitabine HCl for the in vivo experiments was purchased from Selleck Chemicals (S1149; Houston, TX, USA). The antibodies used in this study were against Keap1 (AF5266), MDR1 (AF5185), and MRP5 (DF7149) (Affinity Biosciences, OH, USA); Nrf2 (ab62352), NQO1 (ab80588), HO-1 (ab68477), active caspase-3 (ab2302), and Ki-67 (ab16667) (Abcam Inc., MA, USA); and GAPDH (5174S), Bax (2772S), and Bcl-2 (15071S) (Cell Signaling Technology Inc., MA, USA). Brusatol and Gemcitabine HCl were dissolved in DMSO and then added to medium to a certain concentration while limiting the DMSO concentration to below 0.1%.

Xiang Y., Ye W., Huang C., Yu D., Chen H., Deng T., Zhang F., Lou B., Zhang J., Shi K., Chen B, & Zhou M. (2018). Brusatol Enhances the Chemotherapy Efficacy of Gemcitabine in Pancreatic Cancer via the Nrf2 Signalling Pathway. Oxidative Medicine and Cellular Longevity, 2018, 2360427.

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Anticancer Drug's Effects on Tumor–Stroma Interactions

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To analyze the effects of anticancer drugs on tumor–stroma cross talk, we performed the same experiments by treating the cells with gemcitabine. To this aim, gemcitabine HCl (supplied by Selleckchem) was diluted in DMEM (or in L15 medium just before confocal laser scanning microscopy) at 5 μM final concentration.

Alemanno F., Cavo M., Delle Cave D., Fachechi A., Rizzo R., D’Amone E., Gigli G., Lonardo E., Barra A, & del Mercato L.L. (2023). Quantifying heterogeneity to drug response in cancer–stroma kinetics. Proceedings of the National Academy of Sciences of the United States of America, 120(11), e2122352120.

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Preparation and Formulation of Antineoplastic Agents

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Clofarabine, Cladribine, Gemcitabine HCl, Triapine, Nelarabine, Cisplatin, and LB-100 were purchased from Selleck Chemical. BS-181 and PHA-767491 were purchased from MedChem Express. In vitro use: All compounds except for LB-100 and Cisplatin were reconstituted in DMSO, aliquoted, and stored at −80°C until use. LB-100 was reconstituted to 10 mM in sterile water, aliquoted, and stored at −20°C until use. Cisplatin was reconstituted to 3 mM in sterile saline and stored at 4°C until use. MTT was purchased from Research Products International and reconstituted to 5 mg/mL in sterile PBS, aliquoted, and stored at −20°C until use. In vivo formulation: Clofarabine was prepared for in vivo xenografts in 25% polyethylene glycol 400 (PEG400) (Sigma Aldrich 06855) in 0.9% sterile saline (USP Sterile Grade, Fisher Scientific Z1376).

O’Connor C.M., Taylor S.E., Miller K.M., Hurst L., Haanen T.J., Suhan T.K., Zawacki K.P., Noto F.K., Trako J., Mohan A., Sangodkar J., Zamarin D., DiFeo A, & Narla G. (2022). Targeting ribonucleotide reductase induces synthetic lethality in PP2A-deficient uterine serous carcinoma. Cancer research, 82(4), 721-733.

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