Water soluble 20 kDa chitosan, with a degree of deacetylation of ~70%, from Coyote Foods (Saltillo, CH, Mexico) and Pentasodium Tripolyphosphate (TPP) from Sigma–Aldrich (St. Louis, MO, USA) were weighed and resuspended in MiliQ water, at concentrations of 2 mg/mL and 0.86 mg/mL, respectively. The pH of chitosan was measured and adjusted to 5.5, while for TPP it was adjusted to 3. Cell culture media, fetal bovine serum (FBS), cell culture supplements, and plasmid purification kit were obtained from Invitrogen (Carlsbad, CAL, USA). Iron II sulfate heptahydrate and iron III chloride hexahydrate were purchased from Sigma–Aldrich (St. Louis, MO, USA), ammonium hydroxide was obtained from Productos Quimicos Monterrey (Monterrey, NL, Mexico).
Pentasodium tripolyphosphate
Manufactured by Merck Group
Sourced in United States
Pentasodium tripolyphosphate is a chemical compound that serves as a basic component in various laboratory equipment. It functions as a buffer, chelating agent, and water softener. This inorganic salt is widely used in various industrial and scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Chitosan, by Merck Group (6 mentions)
Acetic acid, by Merck Group (3 mentions)
Lecithin, by Merck Group (2 mentions)
Glibenclamide, by Merck Group (2 mentions)
Taurocholic acid sodium salt, by Merck Group (2 mentions)
Maleic acid, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Silver nitrate, by Merck Group (2 mentions)
Pepsin, by Merck Group (2 mentions)
Acetonitrile, by Tedia (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Glacial acetic acid, by Merck Group (2 mentions)
Medium molecular weight chitosan, by Merck Group (2 mentions)
Plasmid purification kit, by Thermo Fisher Scientific (1 mentions)
Cell culture media, by Thermo Fisher Scientific (1 mentions)
Cell culture supplements, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
15 protocols using pentasodium tripolyphosphate
1
Chitosan-TPP Nanoparticle Synthesis
2
Formulation and Characterization of Pravastatin Sodium Nanoparticles
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Gift sample of Pravastatin Sodium drug was received from Biocon Pvt. Ltd., Bengaluru, India. Chitosan was purchased from HiMedia Pvt. Ltd., Mumbai, India. Glacial acetic acid was purchased from Merck, Mumbai, India. Pentasodium tripolyphosphate and Poloxamer were purchased from Sigma Aldrich, Mumbai, India. All other used chemicals and reagents were of analytical grade.
3
Chitosan-Based Nanoparticles for Bioavailability
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Chitosan (CS, medium molecular weight 190–310 kDa, degree of deacetylation 75–85%), pentasodium tripolyphosphate (TPP, ≥85%), glibenclamide (Gli), silymarin (Sil), formic acid (CH2O2) 98–100%, HPLC-grade methanol and acetonitrile (ACN), taurocholic acid sodium salt (≥95%), lecithin (≥60%), pepsin from gastric porcine mucosa (≥250 units/mg powder), sodium chloride (NaCl; ≥99%), hydrochloric acid (HCl; 37%), maleic acid (≥99%) and sodium hydroxide (≥97%) were purchased from Sigma Aldrich (St. Louis, MO, USA).
4
Curcumin-5-FU Alginate-Chitosan Nanocomposite
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Sodium alginate (SA), chitosan (CS), curcumin (CUR), 5-fluorouracil (5-FU), and pentasodium tripolyphosphate (STPP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Absolute ethanol, calcium chloride, and tween 80 were purchased from Sd. Fine chemicals (Mumbai, India). Millipore water was used throughout the study. rGO was synthesized in accordance with our earlier research (Boddu et al., 2021 ).
5
Nanomaterials Synthesis and Functionalization
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Hexadecyltrimethylammonium bromide for molecular biology (CTAB), ascorbic acid, tetrachloroauric acid (HAuCl4·3H2O), silver nitrate (AgNO3), sodium borohydride (NaBH4), poly(sodium-4-styrenesulfonate) (PSS) of molecular weigh (Mw) ~ 70,000 g/mol, poly-l -lysine hydrobromide (PLL) of Mw ~ 22,000 g/mol, sodium cyanoborohydride (NaCNBH3), octyl aldehyde, human serum albumin, lyophilized powder ≥ 97%, and pentasodium tripolyphosphate (TPP) were from Sigma (Sigma-Aldrich Co.; USA). Doxorubicin hydrochloride (DOXO) was purchased from Calbiochem and docetaxel from Acros Organics. Chitosan with an average molecular weight of 415.000 g/mol and 90% degree of deacetylation (Fluka, Cat. no. 28191, middle viscosity grade) was used. All organic solvents were of HPLC grade and all other chemicals were reagent grade commercially available.
6
Chitosan-based antimicrobial and antiparasitic evaluation
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The chitosan with different molecular weights, low (LMW), medium (MMW), and high (HMW), pentasodium tripolyphosphate (TPP), and acetic acid were purchased from Sigma-Aldrich (St. Louis, MI, USA). The Cinnamomum (C.) verum EO was purchased from a local market in Tunisia, in the form of dried bark strips, as previously described [3 (link)]. Amphotericin B (AMB) was used as a conventional anti-candida and anti-leishmanial treatment.
The bacterial strains including the Gram-negative bacteria (Escherichia coli ATCC 25922 and the clinical strain Salmonella enteritidis DMB 560) and Gram-positive bacteria (Staphylococcus aureus ATCC 6810 and Listeria monocytogenes ATCC 19115), the yeast strain Candida (C.) albicans ATCC 10231, the Leishmanial strains (including L. tropica and L. major), and the murine macrophage Raw 264.7 cell line were obtained from the microorganism collection of the Bioactive Substances Laboratory, CBBC, Tunisia.
The bacterial strains including the Gram-negative bacteria (Escherichia coli ATCC 25922 and the clinical strain Salmonella enteritidis DMB 560) and Gram-positive bacteria (Staphylococcus aureus ATCC 6810 and Listeria monocytogenes ATCC 19115), the yeast strain Candida (C.) albicans ATCC 10231, the Leishmanial strains (including L. tropica and L. major), and the murine macrophage Raw 264.7 cell line were obtained from the microorganism collection of the Bioactive Substances Laboratory, CBBC, Tunisia.
7
Chitosan-based delivery system for Gd-DTPA
8
Synthesis and Characterization of Chitosan Conjugates
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Chitosan hydrochloride (UP CL 113, MW 110 kDa, deacetylation degree 86 %) was purchased from Pronova Biopolymer (Norway). Glycol Chitosan (MW 400 kDa according to supplier instructions), DA hydrochloride, fluorescein isothiocyanate (FITC) and pentasodium tripolyphosphate (TPP) were purchased from Sigma-Aldrich, Italy. Sulfobutylether--cyclodextrin sodium salt (SBE-β-CD, MW 2163 Da, average substitution degree 6.40) was purchased from CyDex, Inc. (USA). All other chemicals were reagent grade. Ultrapure water was used throughout the study. Synthesis and characterization of the conjugates CS-NAC, CS-GSH and GCS-NAC were carried out as previously described [36] .
9
Fabrication and Characterization of Chitosan Nanoparticles
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Chitosan (CS, medium molecular weight 190-310 kDa, degree of deacetylation 75-85%), pentasodium tripolyphosphate (TPP, ≥85%), acetic acid (≥ 99.8%), methanol (≥ 99.8%), glibenclamide (Gly, ≥ 99%) and lipoic acid (LA, ≥ 98%) were purchased from Sigma Aldrich (Merck, Germany). Distilled water of chromatographic purity was purchased from Ircon (Romania). Taurocholic acid sodium salt (≥ 95%), lecithin (≥ 60%), pepsin obtained from gastric porcine mucosa (≥ 250 units/mg powder), sodium chloride (NaCl; ≥ 99%), hydrochloric acid (HCl; 37%), maleic acid (≥ 99%) and sodium hydroxide (≥97%) were also purchased from Sigma Aldrich (Merck, Germany).
10
Chitosan-based Nanoparticle Synthesis and Characterization
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Ethanol (C 2 H 5 OH), acetic acid (CH 3 COOH), titanium tetraisoproxide (TTP), silver nitrate (AgNO 3 ), and chitosan medium molecular weight (Product number 448877, 75-85% deacetylation, 200-800 cP viscosity of 1% w/v in 1% v/v acetic acid), pentasodiumtripolyphosphate (TPP), and glacial acetic acid were all purchased from Sigma-Aldrich (Sigma-Aldrich Co., St Louis, MO, USA). Propidium iodide (PI), Annexin V and in the LIVE/DEAD ® BacLight TM Bacterial Viability Kit (L7012) (ThermoFisher Scientific, UK), MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay kit (Cell Proliferation Kit MTT Sigma-Aldrich), Dulbecco's Modified Eagle Medium (DMEM) (Sigma-Aldrich). The Gram staining chemicals, Nutrient Broth (NB), Nutrient Agar (NA), Muller-Hinton Agar (MHA),MacConkey Agar, Brain-Heart Infusion (BHI) broth, andantibiotic discs were all obtained from Oxoid (Thermo Fisher, England, UK), API 20 E system(France, Biomerieux).
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