Wastewater (500 mL) samples were extracted with 20 ml n-hexane (HPLC grade, SRL, India) and Dichloromethane (DCM) (HPLC grade, SRL, India) using liquid-liquid extraction procedure (APHA, 2005 ). Homogenized wastewater samples were shaken vigorously in separatory funnel thrice, each time using 20 ml n-hexane (HPLC-grade). When the solvent and water layers were separated, the solvent layer was collected in 100 mL amber coloured bottle after separation from the water phase. In case of DCM, acidic and basic fractions were collected by extracting the water samples at pH > 2 and pH < 11 respectively. The extracts were evaporated to dryness and re-constituted in 2 ml of respective solvents and transferred to GC vials and analysed by GC-MS (VARIAN GC-MS-4000), a VARIAN CP-8410 auto sampler and an ion trap mass spectrometer. The system was controlled by a 0 varian star MS work station v6.9.1. The chromatographic column was a Zebron ZB-1701 (30 m 0.25 mm i.d.; 0.15 mm film thickness). The head pressure of the helium carrier gas was at a pressure of 8.7 psi. The sample injection (injection volume 1μL) was made in split mode (having split ratio 10) using a bruker-glass liner. The compounds were identified on the basis of mass spectra using the NIST MS search v2.0 library (National Institute of Standards and Technology).
Cp 8410
Manufactured by Agilent Technologies
Sourced in United States
The CP-8410 is a lab equipment product from Agilent Technologies. It is a compact and versatile instrument designed for sample preparation tasks. The core function of the CP-8410 is to perform automated solid-phase extraction (SPE) and liquid-liquid extraction (LLE) procedures.
Automatically generated - may contain errors
Lab products found in correlation
Gc ms 4000, by Agilent Technologies (2 mentions)
Cp wax 52 cb, by Agilent Technologies (1 mentions)
Gc 3900, by Agilent Technologies (1 mentions)
Cp sil 8 cb capillary column, by Agilent Technologies (1 mentions)
Cp8400, by Agilent Technologies (1 mentions)
Zb wax, by Phenomenex (1 mentions)
Saturn 2200, by Agilent Technologies (1 mentions)
5 protocols using cp 8410
1
Wastewater Organic Contaminant Extraction
2
Serum Free Fatty Acid Profiling
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The serum free fatty acid profile was analyzed by gas chromatography (Varian GC 3900) coupled in flame ionization detection (FID) with a CP-8410 autosampler (Walnut Creek, CA, USA). The formation of fatty acids methyl esters (FAMEs) was performed with acetyl chloride (5% HCl in methanol) [35 ]. Samples were analyzed on a capillary column (CP Wax 52 CB, Varian, Lake Forest, CA, USA); 0.25 µm thickness, inside diameter 0.25 mm and 30 m length). Hydrogen was used as a carrier gas at a linear velocity of 22 cm/s. The temperature program was 170 °C for 1 min, followed by 2.5 °C/min of the increase until 240 °C and a final hold time of 5 min. The temperatures used were 250 and 260 °C for injector and FID, respectively. Fatty acids were identified by comparing the retention time using a known standard of FAMEs. (Supelco, 37 components; Sigma–Aldrich; Mixture, Me93, Larodan and Qualmix, PUFA fish M, Menhaden Oil, Larodan). Data were expressed as percentages of total fatty acids.
3
GC-MS Analysis of Organic Compounds
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The organic compounds were identified by GC-MS (VARIAN GC-MS-4000), a VARIAN CP-8410 auto sampler and ion trap mass spectrometer. The system was controlled by a 0 varian star MS work station v6.9.1. The chromatographic column was a Zebron ZB-1701 (30 m 0.25 mm i.d; 0.15 mm film thickness). The head pressure of the helium carrier gas was 8.7 psi. 1 μL sample was injected in split mode (split ratio 10) using a bruker glass liner. The compounds were identified on the basis of mass spectra using NIST (National Institute of Standards and Technology) MS search library v2.0.
4
GC-MS Analysis of Synthetic Musk Compounds
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Chromatographic analysis of synthetic musk compounds were done in a Varian Ion Trap GC-MS system (Walnut Creek, CA, USA), equipped with a 450-GC gas chromatograph, a 240-MS ion trap mass spectrometer operating in the electron ionization (EI) mode (70 eV), a CP-1177 split/splitless injector and an autosampler model CP-8410. After a 1 μL injection in splitless mode, separation was achieved using a Varian CP-Sil 8 CB capillary column (50 m × 0.25 mm i.d., 0.12 µm) in combination with a FS deactivated pre-column (5 m × 0.530 mm i.d.) from Agilent Technologies (Palo Alto, CA, USA). The oven temperature was programmed as follows: 60 ºC hold for 1 min, raised at 6 ºC min -1 to 150 ºC (hold for 10 min), then 6 ºC min -1 to 225 ºC and finally 20 ºC min -1 to 300 ºC (hold for 2.5 min). The filament emission current was 50 µA, the manifold was maintained at 50 ºC, while the injector, ion trap and transfer line were at 250 ºC. Helium (99.999% purity) was used as carrier gas at 1.0 mL min -1 . Acquisition was made in selected ion storage (SIS) mode and synthetic musks were identified and quantified using retention time and up to three ions with the Varian MS workstation v. 6.9.3 software. The ions monitored for individual compounds have been previously reported (Homem et al., 2013) .
5
GC-MS Analysis of Volatile Compounds
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Volatile compounds were analyzed in a GC (Varian 3800) equipped with an MS detector (Saturn 2200), an automatic sampler (CP-8400) and an autoinjector (CP-8410). The separation was performed on a phenomenex ZB-WAX (30 m × 0.32 mm × 0.50 µm film thickness). The injector temperature was 250 °C and helium was the carrier gas at a flow rate of 1.0 mL/min. The oven programme started at 40 °C (held for 10 min), increased to 100 °C at 15 °C/min (held for 5 min) and finally to 250 °C at 15 °C/ min and held at that temperature for 5 min. The MS was operated in electron ionization mode (70 eV) and scanning was programmed for a m/z range of 29-300. Identification of volatile compounds was achieved by comparison with reference standard, matched spectra from the NIST 2.0 library and fragmentation patterns for compounds reported in the literature. For quantitation of volatiles, stock solutions of standards were dissolved in dichloromethane, and thereafter working concentrations were prepared by diluting to appropriate levels in a model wine solution containing 10 % ethanol, 3.0 g/L malic acid and the pH adjusted to 3.0 with NaOH.
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