The liver and jejunum were embedded in paraffin and sliced (5 μm), and then the sections were stained with hematoxylin and eosin (Hematoxylin-Eosin Staining Kit, Beijing Solarbio Science and Technology Co., Ltd., Beijing China). Sections were photographed with Nikon eclipse 80i microscope (Nikon Instruments, Melville, NY, USA) at 200× magnification.
Hematoxylin eosin staining kit
Manufactured by Solarbio
Sourced in China
The Hematoxylin-eosin (HE) staining kit is a laboratory tool used for staining tissue samples. It consists of two dyes, hematoxylin and eosin, which selectively stain different cellular components, enabling the visualization of tissue structures under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Masson s trichrome stain kit, by Solarbio (5 mentions)
Paraformaldehyde (pfa), by Solarbio (4 mentions)
Streptozotocin (stz), by Merck Group (3 mentions)
Eclipse 80i microscope, by Nikon (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Caspase 3, by Santa Cruz Biotechnology (2 mentions)
Masson s trichrome staining kit, by Solarbio (2 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (2 mentions)
β actin, by Boster Bio (2 mentions)
Nissl staining solution methylene blue, by Solarbio (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
5 ethynyl 2 deoxyuridine edu, by RiboBio (1 mentions)
Z vad fmk, by Abcam (1 mentions)
β catenin, by Santa Cruz Biotechnology (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
64 protocols using hematoxylin eosin staining kit
1
Liver and Jejunum Histological Analysis
2
Diosgenin-Induced Apoptosis Mechanisms
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Diosgenin, RIPA buffer was obtained from Sigma Aldrich (St. Louis, MO, USA). RPMI-1640, DMEM medium, fetal bovine serum (FBS), trypsin, penicillin, and streptomycin were purchased from Gibco (Rockville, MD, USA). PI (propidium iodide), Annexin V-FITC/PI apoptosis detection kit, z-VAD-fmk, MTS, Hoechst 33258, and AO/EB (acridine orange/ethidium bromide) were purchased from Abcam (Cambridge, United Kingdom). 5-Ethynyl-2-deoxyuridine (EdU) was obtained from RiboBio (Guangzhou, China). The primary antibodies against GAPDH, Bax, Bcl-2, Caspase- 3, p21, PARP-1, cytochrome c (CYT C), COX IV, β-catenin and GSK3β were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Other antibodies were from Abcam. Hematoxylin–Eosin Staining Kit was from Solarbio (Beijing, China). The EliVision kit was from Maixin Biotech (Fuzhou, China).
3
Boyden Chamber Assay for Cell Migration and Invasion
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The Boyden chamber consists of two compartments separated by a polycarbonate membrane containing uniformly sized pores of 8.0 µm. The Boyden chamber assay is a widely accepted and useful technology to study chemotaxis of leukocytes or other migratory cells. For the Boyden chamber migration assay, 200 µl serum-free medium containing 2×105 cells was placed in the upper compartment and complete medium with 20% FBS was added to the lower compartment. After conventional incubation for 12 h, the cell source chamber was removed, and the excess cells on the upper side of the filter (those that did not migrate across the filter) were removed by gently wiping. The entire filter was then submerged in a fixation solution (4% paraformaldehyde) for 30 min and stained using a Hematoxylin–Eosin staining kit (Solarbio). Using a microscope set at 200× magnification, the number of stained cells in 5 fields on the lower side of the filter were enumerated, and the average count was calculated. For the Boyden chamber invasion assay, the microporous filter was coated with Matrigel (BD BioCoat) to form a bioactive three-dimensional matrix, and the physical characteristics and function of the cell basement membrane were simulated. The procedure was the same as that of the migration assay, except for the incubation time, which was 24 h.
4
Histological Assessment of Cardiac Hypertrophy
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Mice were anesthetized with 5% isoflurane, and hearts were excised 3-4 weeks after TAC surgery. The death of mice was confirmed by lack of breathing and lack of a heartbeat. Before fixing with 4% paraformaldehyde, the heart was perfused with 10 mL cold PBS. Then, the heart was embedded in OCT compound; 7-μm-thick sections were used for staining. Hematoxylin and eosin staining were performed using a Hematoxylin-eosin Staining Kit (Beijing Solarbio Science & Technology Co., Ltd., Cat. #: G1121) to observe the global changes in heart size. After incubation with hematoxylin solution, sections were washed and differentiated, followed by incubation in eosin solution, dehydrated in a series of ethanol solutions (75%-100%), then cleared in xylene and mounted in neutral gum. Masson's trichrome was used to assess collagen density using a Masson's Trichrome Staining Kit (Beijing Solarbio Science & Technology Co., Ltd. Cat. #: G1346) according to the manufacturer's instructions. To evaluate the cardiomyocyte cross-sectional area, FITC labeled Wheat Germ Agglutinin and Alexa Fluor 647 labeled WGA (Thermo Fisher Scientific, Cat. #: W834 and W32466) were used according to the manufacturer's instructions.
5
Histological Analysis of Liver Necrosis
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Hematoxylin and eosin (H&E) staining was used to assess the necrosis area of the liver. Mouse liver tissues were fixed in 10% formalin, embedded in paraffin and sectioned to 5 μm slides. The slides were deparaffinized and stained by a Hematoxylin-Eosin Staining Kit (Solarbio) according to the manufacturer's protocol. For IHC staining, liver sections were subjected to deparaffinization and antigen retrieval at first, then the non-specific antibody binding was blocked by using 10% bovine serum albumin (BSA; Biosharp) at room temperature for 1 h. After incubating with primary antibodies of FGF10 (ABN44, Millipore, 1:1000 dilution), Ki-67 (12202, Cell Signaling Technology, 1:400 dilution), CD68 (sc-20060, Santa Cruz Biotechnology, 1:50 dilution), or MPO (ab9535, Abcam, 1:50 dilution) at 4 °C overnight, appropriate secondary antibodies conjugated with HRP were added and incubated at room temperature. A Metal Enhanced DAB Substrate Kit (Solarbio) was used to visualize the sections followed by hematoxylin counterstaining. All images were captured by using a Nikon ECLIPSE Ni microscope.
6
SRNS Mice Treatment with GC, H, GCH, Pm-GCH
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Then, 100 μl GC, H, GCH and Pm-GCH (H, 1 mg/ml) were injected daily through the tail vein of SRNS mice for 3 consecutive days. Urine was collected for 24-h urinary protein quantitation. The concentration of urine protein was detected with a BCA Protein Assay Kit (P0012S, Beyotime, China). After 12 days, all SRNS mice were euthanized, and fresh whole blood was taken for routine blood and kidney function. Hematological indices (RBCs, Hb, WBCs, PLTs) were determined by an automatic blood cell analyzer (Mindray, China). The liver function index (ALT, AST) and renal function index (CREA, BUN) were detected by a Leadman Diagnostic Kit (Leadmanbio, China). Kidney, lung, liver, spleen, heart and femur samples were fixed with 4% paraformaldehyde and embedded in paraffin. Ultrathin sections were used for HE and Masson staining with a hematoxylin–eosin staining Kit (G1120, Solarbio, China) and Masson’s trichrome stain kit (G1340, Solarbio, China).
7
Histological Analysis of Mouse Lung Tissue
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The left lung tissue of mice was fixed with 4% PFA for 2 days, embedded in paraffin and sectioned. After deparaffinization and gradient alcohol hydration, paraffin tissue sections (5 μM) were stained with the Hematoxylin-Eosin Staining Kit or Masson Trichrome Stain Kit (Solarbio Life Science, Beijing, China). For immunohistochemistry, a 3%-hydrogen-peroxide solution was added dropwise to tissue sections to quench endogenous peroxidase activity. The sections were then immersed in 0.01 M citrate (Solarbio Life Science, Beijing, China) buffer, and antigen retrieval was performed by the microwave method. Normal goat serum (10%) was added, and the sections were incubated for 1 h at room temperature to block nonspecific binding. The blocking solution was removed, a primary antibody was added dropwise, and the sections were incubated overnight at 4 °C. A secondary antibody working solution was added dropwise and incubated at room temperature for 1 h. A freshly prepared DAB working solution (Solarbio Life Science, Beijing, China) was dropped onto slides, and the degree of staining was monitored under a microscope. Finally, the sections were counterstained with hematoxylin for 2 min. The stained tissue sections were observed under a microscope (Leica, Wetzlar, Germany).
8
Histological Analysis of Brown Adipose Tissue
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Partial BAT was fixed in 4% formaldehyde in PB (pH 7.4) for 24 h. After dehydration with various concentrations of ethanol and clarifying with xylene, BATs were embedded in mixed paraffin prior to sectioning. BATs were cut into 5-μm sections using a paraffin slicing machine (RM2016; Leica, Wetzlar, Germany). Sections were stained using a Hematoxylin-Eosin Staining Kit (G1120; Solarbio, Beijing, China). Cell numbers were analyzed using ImagePro-Plus software.
9
Quantitative Lung Collagen Analysis
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The mouse right lung lobe and human lung tissue were fixed with 4% neutral paraformaldehyde for 24 h. Tissues were embedded in paraffin and sectioned (4 μm). The sample slides were stained with trichrome stain (Masson’s) kits (D026-1-2, Nanjing Jiancheng Bioengineering Institute, China) to detect collagen deposition according to the instructions. Next, a photograph documentation facility examined the slides under a light microscope. Using ImageJ software, collagen content was measured by the ratio of collagen’s surface area (blue) to the total surface area (red).
H&E staining was conducted according to the Hematoxylin-Eosin Staining Kit (Solarbio, G1120) manufacturer’s instructions.
H&E staining was conducted according to the Hematoxylin-Eosin Staining Kit (Solarbio, G1120) manufacturer’s instructions.
10
Mesenchymal Stem Cell Characterization
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TGF-β1 (PEPRO TECH, China, 100-21-10 μg), PC-MSC (Provided by Shenzhen 150 Biomedical Co.,Ltd.), ESC (Provided by ATCC), FITC Mouse IgG1 κ Isotype control (Biolegend, 7313762), PE Mouse IgG1 κ Isotype control (Biolegend, 9217868), APC Mouse IgG1 κ Isotype control (Biolegend, 9282592), PE Mouse Anti-Human CD73 (Biolegend, 8151901), APC Mouse Anti-Human CD105 (Biolegend, 9277133), FITC Mouse IgG2a κ Isotype control (Biolegend, 8241935), FITC Mouse Anti-Human CD14 (Biolegend, 8299630), FITC Mouse Anti-Human CD45 (Biolegend, 8206704), DMEM/F12 medium (Peiyuan, Shanghai, L310 kJ), Fetal bovine serum (Gemini, 900-108), Trypsin digestive fluid (Beyo, C0203), DPBS (Beyo, C0221G), CCK8 (Beyotime, C0039), EDU staining kit (Beyotime, C0081s), Hematoxylin-eosin staining kit (Solarbio, G1120), DEPC-treated water (Solarbio, R1600), trichloromethane (Sinopharm Chemical Reagent Co. LTD., 10006818), GelRed dye (Biotium, #41003), RNA Loading Buffer (TaKaRa, 9168), Tris-Borate-EDTA Buffer (TBE) 10× Powder, pH8.3 (TaKaRa, T9122), RNAsimple Total RNA Kit (TIANGEN, DP419), All-in-One First-Strand Synthesis Master Mix (Yugong Biolabs, EG15133S), SuperReal PreMix Plus (SYBR Green) (TIANGEN, FP205-02), and Methacrylate anhydride (MAA, 0.1 mL/1 g gelatin), ELISA Kit (Solarbio, SEKH-0052), MateRegen® Gel (2104006, BioRegen Biomedical (Changzhou) Co., Ltd.).
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