Hplc grade acetone

Manufactured by Merck Group

Sourced in Germany, United States, Spain, Poland, United Kingdom, India, Belgium

HPLC grade acetone is a highly purified solvent used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile, and flammable liquid with a characteristic odor. HPLC grade acetone is designed to meet the stringent purity requirements of HPLC analysis, ensuring consistent and reliable results.

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Lab products found in correlation

Dichloromethane, by Merck Group (6 mentions) Hydrochloric acid (hcl), by Merck Group (6 mentions) Methanol, by Merck Group (6 mentions) Sodium chloride (nacl), by Merck Group (6 mentions) Acetonitrile, by Merck Group (5 mentions) Formic acid, by Merck Group (5 mentions) Ethyl acetate, by Merck Group (5 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) N hexane, by Merck Group (4 mentions) Toluene, by Merck Group (4 mentions) Whatman, by Cytiva (3 mentions) Sodium hydroxide (naoh), by Merck Group (3 mentions) Sodium carbonate, by Merck Group (3 mentions) Citric acid, by Merck Group (3 mentions) Acetic acid, by Merck Group (3 mentions) Hplc grade methanol, by Merck Group (3 mentions) Ethanol, by Merck Group (3 mentions) Hplc grade acetonitrile, by Merck Group (3 mentions) Lc ms grade acetonitrile, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Acetone (2462 citations) HPLC grade (319 citations) HPLC/Spectro grade acetone (2 citations)

69 protocols using hplc grade acetone

Inhibition of Mild Steel Corrosion

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Commercially available inhibitors (imidazole (IM), 2-ethylimidazole (EI), thiophene (TH), 3-methoxythiophene (MT), 2-ethylthiophene (ET), pyridine (PY), 4-ethylpyridine (EP), 4-methoxypyridine (MP)) were prepared in 0.01- and 10.0-mM concentrations by dissolving in 1 M HCl (Figure 1). The electrolyte solution of 1 M HCl was prepared from analytical grade 12 M (ACS reagent 37% Sigma-Aldrich) HCl.
Low-carbon mild steel (MS) samples were characterized using an optical emission spectrometer (OES) (Spectro Lab Lav M11). Table 6 describes the composition of the samples. The surface of the substrate was abraded using 240, 600, 1000, and 2000 grit emery papers. HPLC-grade acetone (≥99.9%, Sigma-Aldrich) was used to wash and degrease the mild steel samples followed by air-drying in a dry box before use.Table 6

Elemental composition of the mild steel used in the study.

Table 6

% C	% Si	% Mn	% P	% S	% Cr	% Mo	% Ni	% Cu	% Fe
0.0613	0.0107	0.2700	0.0140	0.0059	0.0343	0.0170	0.0247	0.0120	bal

Carranza M.S., Reyes Y.I., Gonzales E.C., Arcon D.P, & Franco FC J.r. (2021). Electrochemical and quantum mechanical investigation of various small molecule organic compounds as corrosion inhibitors in mild steel. Heliyon, 7(9), e07952.

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Metabolite Confirmation using HPLC-MS

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HPLC grade acetone was purchased from Sigma Aldrich (Dorset, UK). LC-MS grade acetonitrile, water, methanol and chloroform were purchased from Fisher Scientific (Loughborough, UK). All analytical standards for metabolite confirmation were purchased from Sigma Aldrich.

Gray D.W., Welsh M.D., Doherty S., Mansoor F., Chevallier O.P., Elliott C.T, & Mooney M.H. (2015). Identification of systemic immune response markers through metabolomic profiling of plasma from calves given an intra-nasally delivered respiratory vaccine. Veterinary Research, 46, 7.

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Posaconazole Formulation Development

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Posaconazole was manufactured by Merck & Co. Inc. (Kenilworth, NJ, USA). HPLC-grade acetone (Sigma-Aldrich, St. Louis, MO, USA), HPMCAS MF grade (Shin-Etsu Chemical Co., Totowa, NJ, USA) were purchased. All materials were used as received.

Li Y., Mann A.K., Zhang D, & Yang Z. (2021). Processing Impact on In Vitro and In Vivo Performance of Solid Dispersions—A Comparison between Hot-Melt Extrusion and Spray Drying. Pharmaceutics, 13(8), 1307.

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Textile Matrix Lipid Extraction and Analysis

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Samples were prepared according to.¹² (link) Specifically, 35 mg of textile matrix was weighed per sample. Afterward, 2 mL of HPLC-grade acetone (Sigma Aldrich, Sydney, Australia) and 2 mL of HPLC-grade chloroform (ChemSupply, Port Adelaide, Australia) were added to each vial and sonicated, without heat, for 20 minutes. The samples were then left at 4 °C for 16 hours for extraction. Afterward, 1 mL of the resulting extract was filtered through a 0.2 μm hydrophobic PTFE syringe filter (MicroAnalytix, Taren Point, Australia). Then, 25 μL of the filtered aliquots were spiked with 20 μL 10 ppm stearic acid-d3 (Merck, Macquarie Park, Australia) and 50 μL of 10 ppm cholesterol-d7 were added as internal standards. These were dried down under a gentle stream of nitrogen at 40°C. Samples were then reconstituted in 40 μL of N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) with trimethylchlorosilane (TMCS) (PM Separations, Capalaba, Australia) and 10 μL of HPLC acetonitrile (Labscan Ltd., Gillman, Australia), and heated for 30 minutes at 70°C prior to being transferred into 250 μL polymer inserts (Agilent Technologies, Mulgrave, Australia). All samples were extracted and aliquoted in duplicates and injected onto the GC-MS/MS, where duplicate runs were conducted, giving a total of four data points per sample with the addition of a full scan injection for quality control purposes.

Collins S., Maestrini L., Hui F.K., Stuart B, & Ueland M. (2023). The use of generalized linear mixed models to investigate postmortem lipids in textiles. iScience, 26(8), 107371.

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Carbonyl Compound Analysis by HPLC-DNPH

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2,4-dinitrophenylhydrazine (DNPH), high performance liquid chromatography (HPLC)-grade acetone, dichloromethane, methanol (MeOH), and water were purchased from Sigma-Aldrich (St. Louis, MO). HPLC-grade hexane, acetone, and ACS-grade hydrochloric acid (HCl) were purchased from Fisher Scientific (Fair Lawn, NJ). Silica gel 60 thin layer chromatography plates (aluminum sheets, 20 × 20 cm, 200 μm) were purchased from EMD Millipore (Billerica, MA). Nylon membrane syringe filters (0.45 μm) were purchased from Whatman Ltd. (Maidstone, Kent, England). LC-MS-grade water and acetonitrile (ACN) were purchased from Thermo Fisher Scientific (Houston, TX) and ammonium acetate from Honeywell Riedel-de Haen (Seelze, Germany).

Fiecke C., Kurzer M., Chen C, & Csallany A.S. (2019). The in Vivo Antioxidant Effects of (−)-Epigallocatechin-3-Gallate Consumption in Healthy Postmenopausal Women Measured by Urinary Excretion of Secondary Lipid Peroxidation Products. Food and nutrition sciences, 10(1), 15-27.

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Carbonyl Compound Analysis by HPLC-DNPH

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Encapsulation of Methotrexate in TPGS-PLGA Nanoparticles

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D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS1000), Poly (D, L-lactide-co-glycolide) (PLGA), methotrexate (MTX), distilled water, HPLC grade acetone, ethanol and acetonitrile, mannitol were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). A simulated gastrointestinal environment (at buffer pH 1.2 and 7.4) was prepared from analytical grade reagents according to the method reported by Sardo et al., 2019 [41 (link)]. All of the reagents procured were of the highest analytical grade. Alginic acid sodium salt from brown algae (sodium alginate), methotrexate, phosphate-buffered saline, hydrochloric acid, gelatine powder, and calcium chloride dihydrate were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). All of the reagents procured were of the highest analytical grade.

Kondiah P.P., Rants’o T.A., Makhathini S.S., Mdanda S, & Choonara Y.E. (2022). An Oral 3D Printed PLGA-Tocopherol PEG Succinate Nanocomposite Hydrogel for High-Dose Methotrexate Delivery in Maintenance Chemotherapy. Biomedicines, 10(7), 1470.

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Anhydrous Acetone Preparation for Reaction

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HPLC grade acetone, 3Å molecular sieve (4–8 mesh), and 98% 4,4’-methylene diphenyl diisocyanate were acquired from Sigma-Aldrich (St. Louis, MO). Fatal-Plus^® (sodium pentobarbital) euthanasia solution was acquired from Vortech Pharmaceuticals, Ltd. (Dearborn, MI). Dry acetone was prepared by incubating 10 ml HPLC grade acetone on 3 Å molecular sieve for a minimum of 24 hours to adsorb water.

Lin C.C., Law B.F., Siegel P.D, & Hettick J.M. (2018). Circulating miRs-183-5p, -206-3p and -381-3p may serve as novel biomarkers for 4,4’-methylene diphenyl diisocyanate exposure. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 24(1), 76-90.

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Extraction and Characterization of Peanut Polyphenols

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A. hypogaea roots were provided by the local farmer from Shandong Province, China, and identification was authenticated by Haifang Xiao from SDUT. The chemicals used were of analytical grade or higher. Folin-Ciocalteu reagent, gallic acid, sodium carbonate (anhydrous), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) or ABTS, anhydrous aluminum chloride, sodium nitrite (NaNO₂), calcium carbonate, copper (II), (+)-catechin, vanillin, ascorbic acid, HCl, NaOH, quercetin, trolox, DPPH (2,2-diphenyl-1-picrylhydrazyl), potassium persulphate, and potassium phosphate were purchased from Sigma-Aldrich (Steinheim, Germany). Formic acid was purchased from Merck (Darmstadt, Germany), and HPLC-grade acetone, ethanol, methanol, and acetonitrile were supplied by Sigma-Aldrich (Steinheim, Germany).

Ullah S., Hussain S.A., Shaukat F., Hameed A., Yang W, & Song Y. (2019). Antioxidant Potential and the Characterization of Arachis hypogaea Roots. BioMed Research International, 2019, 7073456.

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Preparation of E2 Stock Solutions

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E2 (>99% pure), purchased from Sigma–Aldrich and dissolved at a concentration of 1000 mg L⁻¹ in acetone, were used as stock solutions. The organic solvents, methanol, ethyl acetate, acetonitrile, and HPLC-grade acetone were also obtained from Sigma–Aldrich. All other chemicals were of the highest commercially available purity.

Li M., Zhao X., Zhang X., Wu D, & Leng S. (2018). Biodegradation of 17β-estradiol by Bacterial Co-culture Isolated from Manure. Scientific Reports, 8, 3787.

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