Mp e 65 mm lens

All specimens in the collections at the Swedish Museum of Natural History were collected with Malaise Traps. Five specimens of A.lituratus were collected within the Swedish Malaise Trap Project (SMTP). Two specimens were collected by A. Ohlsson in 2011. The single specimen of A.muricatus was collected in a allotment garden by T. Malm and M. Malm in 2012. Terminology used for morphological and wing structures follow Wharton et al. (1997) and Goulet and Hubert (1993) (in parenthesis). Institutional abbreviations: National Museum Ireland (Dublin, Irland) – NMI, Taiwan Agriculture Research Institute (Wufeng, Taiwan) – TARI, Zoological Institute, Russian Academy of Scienses (St. Petersburg, Russia) – ZIN, Swedish Museum of Natural History (Stockholm, Sweden) – NHRS, Swedish Malaise Trap Project – SMTP. For molecular methods regarding the mitochondrial marker CO1 see Stigenberg et al. (2015) (link). Sequences were assembled, edited and imaged using Geneious version 8.1 created by Biomatters. Voseq 1.7.3 (Peña and Malm 2012 (link)) database was used for storing voucher and DNA sequence data. Sequences are deposited at GenBank under the accession numbers: KU521563 to 65, with an additional sequence KJ591423 from an earlier publication (Stigenberg et al. 2015 (link)). Images were taken using Canon EOS D50 with a MP-E 65 mm lens. The images were stacked using Zerene Stacker software.

We used a Canon EOS Rebel T3i equipped with a Canon MP-E 65 mm lens for macrophotography. Illumination was provided by two external flashlights with polarizing filters. We took multiple images in z-stacks at different focal levels. These images were later fused into one focal image using CombineZM (1.0.0). Color balance, saturation and sharpness were adjusted using Photoshop (Ver. 14.0).

The type series of Tomolanguria gen. nov., Anadastusornatus Arrow, 1925, A.pulchellus Arrow, 1925, A.jucundus (Gorham, 1903), Clerolanguriatricolor (Fabricius, 1787), and Stenolanguriatricolor Fowler, 1885 are deposited in the Natural History Museum, London, United Kingdom (BMNH). The holotypes of Paederolanguriaholdhausi Mader, 1939 and P.klapperichi Mader, 1955 are deposited in the Naturhistorisches Museum, Basel, Switzerland (NHMB). The holotype of Paederolanguriaalternata (Zia, 1959) and non-type specimen of P.holdhausi are deposited in the Institute of Zoology of the Chinese Academy of Sciences, Beijing, China (IZCAS). The holotype of Stenolanguriarobusta Villiers, 1958 is deposited in the Muséum national d’Histoire naturelle, Paris, France (MNHN). Label data are given with separate lines on labels indicated by / and separate labels by //. Other comments and remarks are in square brackets [].
All photographs were taken with a Canon 5D Mark III digital camera equipped with a Canon MP-E 65 mm lens. The images were stacked with Helicon Focus 6.7.1 and modified in Adobe Photoshop CS6 to correct for contrast, brightness, and imperfections.
Body length was measured from the apices of mandibles to the apices of elytra.

Specimens examined were obtained from or deposited in the collections of the following institutions:
ZISP

Zoological Institute of the Russian Academy of Sciences, Saint-Petersburg, Russia

ZMUM

Zoological Museum of the Lomonosov State University, Moscow, Russia

MNKB

Museum für Naturkunde, Leibniz-Institut für Evolutions und Biodiversitätsforschung, Berlin, Germany

Morphological terminology and abbreviations generally follow White et al. (1999) . Series of photos were taken directly by the Canon EOS 40D camera with Canon MP-E 65 mm lens and then composed with CombineZM software (Hadley 2007 ). Measurements are provided in millimetres.

NHMWNaturhistorisches Museum Wien, Vienna, AustriaOUMNHOxford University Museum of Natural History, Oxford, UKSKYC Seiki Yamane Collection, Kagoshima, Japan
ZRCZoological Reference Collection, Lee Kong Chian Natural History Museum, SingaporeSource images for focus stacking were taken using a Canon EOS Kiss X9 digital camera, attached to a Nikon AZ100 stereomicroscope (for O.litoralis worker, queen, and male bodies of all species, excluding male genitalia), and a Nikon Eclipse E600 microscope (for male genitalia). Focus-stacked images were produced using Helicon Focus Pro 7.0.2 (Helicon Soft Ltd., http://www.heliconsoft.com/), and improved with the retouching function of the same software. Colour balance and contrast were adjusted using GIMP 2.8 (The GIMP Development Team, http://www.gimp.org).
Workers of O.malignus were imaged with a Dun Inc. Passport II macrophotography imaging system, using a Canon MP-E 65 mm lens; focus-stacked images were produced using Zerene Stacker v1.04 (Zerene Systems LLC, https://zerenesystems.com/cms/stacker). All final images were further adjusted, annotated, and scale bars added using Adobe Photoshop CS6.

The specimens were collected in northwestern China from July to August 2016–2017 using sweep netting, light trapping, and Malaise trapping. The specimens were studied and illustrated with a ZEISS Stemi 2000-c stereomicroscope; the photo illustrations were taken under a Canon Mark IV with Canon MP-E 65 mm lens. The descriptions were based on specimens preserved in 95% alcohol. Genitalic preparations of males were made by macerating the apical abdomen in cold 10% NaOH for 12–15 hours. After examination, preparations were transferred to fresh glycerin for preservation and stored in a microvial pinned below the specimen. The morphological terminology mainly follows McAlpine (1981) . Type specimens are deposited in the Entomological Museum of China Agricultural University (CAU), Beijing, China.

We took stacking images with a Canon EOS 7D with a MPE 65mm lens (Canon, Tokyo, Japan). We used Helicon Focus Version 7 (Helicon Soft Ltd., Kharkiv, Ukraine) to focus stack multiple images, and added a scale and brightness adjustments with Adobe Photoshop CS6 13.0 (Adobe Inc., San Kaso, CA, USA). All images presented are available online and can be viewed on AntWeb (Antweb 2020 (link)), where it can be identified by a specimen-level code affixed to the pin.