Agilent 1200 hplc 6520 qtof ms

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1200 HPLC/6520 QTOF-MS is a high-performance liquid chromatography (HPLC) system coupled with a quadrupole time-of-flight mass spectrometer (QTOF-MS). The HPLC component is designed for efficient separation of complex mixtures, while the QTOF-MS provides accurate mass measurements and high-resolution mass spectral data.

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Lab products found in correlation

Agilent 1200 series hplc, by Agilent Technologies (2 mentions) Avance 3 500 spectrometer, by Bruker (2 mentions) Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (2 mentions) Genesys 10 s uv vis spectrometer, by Thermo Fisher Scientific (2 mentions) Agilent 1260 infinity 2, by Agilent Technologies (2 mentions) Triart c18, by YMC (2 mentions) Mcp 200 polarimeter, by Anton Paar (2 mentions) Avance drx 500 spectrometer, by Bruker (1 mentions) Sephadex lh 20, by GE Healthcare (1 mentions) J 815 spectropolarimeter, by Jasco (1 mentions) Cary 50 spectrophotometer, by Agilent Technologies (1 mentions) Avance drx600 spectrometer, by Bruker (1 mentions)

4 protocols using agilent 1200 hplc 6520 qtof ms

Spectroscopic Characterization of Organic Compounds

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The CD spectrum was recorded on a JASCO J-815 Spectropolarimeter, using CHCl₃ as solvent. The NMR spectra were obtained on a Bruker Avance DRX500 spectrometer. Chemical shifts were calibrated internally against residual solvent signals (DMSO‑d₆: δ_C 39.5, δ_H 2.50; Acetone-d₆: δ_C 206.7, 29.9, δ_H 2.05). HRESIMS measurements were obtained on Agilent 1200 HPLC/6520 QTOF-MS. Sephadex LH-20 (GE) was used for purification. HPLC was performed using Agilent 1200 Series HPLC controlled using ChemStation Rev.B.02.01.

Zhang J., Wang Z., Song Z., Karthik L., Hou C., Zhu G., Jiang L., Han J., Ma R., Li L., Zhang L., Liu X, & Hsiang T. (2019). Brocaeloid D, a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049. Synthetic and Systems Biotechnology, 4(4), 173-179.

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Analytical Characterization of Compounds

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Optical rotations were measured with an Anton Paar MCP 200 polarimeter with a sodium lamp (589 nm) (Anton Paar GmbH, Graz, Austria). UV spectra were obtained on Genesys 10S UV-Vis spectrometer (Thermo Fisher Scientific Ltd, Waltham, MA, USA); IR spectra were recorded with a Nicolet IS5 FT-IR spectrometer (Thermo Fisher Scientific Ltd, Waltham, MA, USA); NMR spectra were recorded on Bruker AVANCE III 500 spectrometer (Bruker Inc., Karlsruhe, Germany). HPLC-MS were acquired on Agilent 1200HPLC/6520QTOFMS (Agilent Technologies Inc., Santa Clara, CA, USA). Semi-preparative HPLC isolation was performed on Agilent 1260 Infinity II (Agilent Technologies Inc., Santa Clara, USA) with an ODS column (YMC-Triart C18, 10 mm × 250 mm, YMC Co. Ltd., Tokyo, Japan). Silica gel (200–300 and 300–400 mesh) used in column chromatography (CC) and silica gel GF₂₅₄ (10–40 µm) used in thin layer chromatography (TLC) were supplied by Qingdao Marine Chemical Factory in China.

Peng A., Qu X., Liu F., Li X., Li E, & Xie W. (2018). Angucycline Glycosides from an Intertidal Sediments Strain Streptomyces sp. and Their Cytotoxic Activity against Hepatoma Carcinoma Cells. Marine Drugs, 16(12), 470.

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Spectroscopic Analysis of Tanshinone IIA

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The optical rotation was measured using a Perkin-Elmer Model 343 polarimeter with a 5 cm cell. The UV–vis spectra were obtained on a Cary 50 spectrophotometer. IR spectrum was recorded on a Nicolet 5700 FT-IR Microscope spectrometer (FT-IR Microscope Transmission). The NMR spectra were obtained on a Bruker Avance DRX600 spectrometer. Chemical shifts were calibrated internally against residual solvent signals (DMSO-d₆: δ_C 39.5, δ_H 2.50). HRESIMS measurements were obtained on Agilent 1200 HPLC/6520 QTOF-MS. ODS-A (Octadecylsilyl-A, YMC, 150 μm, AA12SA5) were used for purification. HPLC was performed using Agilent 1200 Series HPLC controlled using ChemStation Rev.B.02.01. Energy minimization (MM2) of structures was performed using Chem3D 12.0. (Cambridge Soft). Tanshinone IIA was purchased from Xi'an Hao-Xuan Bio-Tech Co., Ltd.

He W., Liu M., Huang P., Abdel-Mageed W.M., Han J., Watrous J.D., Nguyen D.D., Wang W., Song F., Dai H., Zhang J., Quinn R.J., Grkovi T., Luo H., Zhang L, & Liu X. (2016). Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation. Synthetic and Systems Biotechnology, 1(3), 187-194.

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Phytochemical Characterization of Natural Compounds

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Silica gel (200–300 mesh), used in column chromatography (CC), and silica gel GF₂₅₄ (10–40 µm), used in thin layer chromatography (TLC), were purchased from Qingdao Marine Chemical Factory in China. Optical rotations were recorded with an Anton Paar MCP 200 polarimeter with a sodium lamp (589 nm) (Anton Paar GmbH, Graz, Austria). UV spectra were measured on a Genesys 10S UV-Vis spectrometer (Thermo Fisher Scientific Ltd., Waltham, MA, USA); IR spectra were recorded with a Nicolet IS5 FT-IR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA); 1D and 2D NMR spectra were recorded on a Bruker AVANCE III 500 spectrometer (Bruker Inc., Karlsruhe, Germany). HPLC-HR-ESI-MS was performed on an Agilent 1200HPLC/6520QTOFMS (Agilent Technologies Inc., Santa Clara, CA, USA). Semipreparative HPLC isolation was conducted on an Agilent 1260 Infinity II (Agilent Technologies Inc., Santa Clara, CA, USA) with an ODS column (10 × 250 mm, YMC-Triart C18, YMC Co. Ltd., Tokyo, Japan).

Qu X.Y., Ren J.W., Peng A.H., Lin S.Q., Lu D.D., Du Q.Q., Liu L., Li X., Li E.W, & Xie W.D. (2019). Cytotoxic, Anti-Migration, and Anti-Invasion Activities on Breast Cancer Cells of Angucycline Glycosides Isolated from a Marine-Derived Streptomyces sp.. Marine Drugs, 17(5), 277.

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