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2 protocols using terpinen 4 ol

1

Immune Cell Calcium Signaling Assay

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Dimethyl sulfoxide (DMSO), N-formyl-Met-Leu-Phe (fMLF), phorbol 12-myristate 13-acetate (PMA), Histopaque 1077, α-terpineol, myrtenol, and γ-terpinene were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Geraniol, germacrene D, α-humulene, and β-caryophyllene were from Cayman Chemicals (Ann Arbor, MI, USA), and (-)-terpinen-4-ol was purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). n-Hexane was purchased from Merck (Darmstadt, Germany). Fluo-4AM was purchased from Invitrogen (Carlsbad, CA, USA). L-012 was purchased from Tocris Bioscience (San Diego, CA, USA). Hanks’ balanced salt solution (HBSS; 0.137 M NaCl, 5.4 mM KCl, 0.25 mM Na2HPO4, 0.44 mM KH2PO4, 4.2 mM NaHCO3, 5.56 mM glucose, and 10 mM HEPES, pH 7.4) was purchased from Life Technologies (Grand Island, NY, USA). HBSS without Ca2+ and Mg2+ is designated as HBSS; HBSS containing 1.3 mM CaCl2 and 1.0 mM MgSO4 is designated as HBSS+.
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2

Characterization of Essential Oils

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Essential oils of patchouli (Pogostemon patchouli), Salvia stenophylla, and Thymus zygis were purchased from Oshadhi (Bühl/Baden, Germany, www.oshadhi.eu). Hyssop (Hyssopus officinalis), litesa (Litsea cubeba), lavender (Lavandula officinalis), majoram (Origanum majorana L.), and sage spanish (Salvia officinalis), were purchased from JinArome (Anyang, Korea, www.jinarome.com). α-Pinene (purity, 99%), camphene (80%), β-pinene (99%), p-cymene (99%), 1,8-cineole (99%), limonene (97%), camphor (99%), and α-humulene (96%) were obtained from Sigma-Aldrich (Milwaukee, WI, USA). α-Terpinene (85%), and sabinene hydrate (97%) were purchased from Fluka (Buchs, Switzerland). (−)-α-Phellandrene (65%), terpinolene (85%), (±)-terpinen-4-ol (95%), and β-caryophyllene (90%) were obtained from the Tokyo chemical industry (Tokyo, Japan). Acetone was purchased from Merck (Darmstadt, Germany) (99.8%).
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