Brain derived neurotrophic factor bdnf

Manufactured by Merck Group

Sourced in Japan

Brain-derived neurotrophic factor (BDNF) is a protein found in the human body that plays a crucial role in the growth, development, and survival of nerve cells. It is an important factor in the nervous system and is involved in the regulation of synaptic transmission and plasticity.

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Lab products found in correlation

Fluorescence microscope, by Olympus (1 mentions) Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions) Retinoic acid, by Merck Group (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Wisent (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) L glutamine, by Merck Group (1 mentions) Geneticin, by Thermo Fisher Scientific (1 mentions) Retinoic acid, by Bio-Techne (1 mentions)

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BDNF (149 citations)

3 protocols using brain derived neurotrophic factor bdnf

GAP-43 Regulates BMSC Differentiation

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To analyze the effects of the overexpression of GAP-43 on BMSC differentiation, the third passage BMSCs were divided into three groups: Blank, NC (transduced with negative control lentiviral vector) and GAP-43 (transduced with LV5-GAP-43). The expression levels of GAP-43, NSE, nestin, NF and βIII-tubulin were detected using semi-quantitative PCR and western blotting. The morphology of the BMSCs in each group was observed under a fluorescence microscope (Olympus Corp., Tokyo, Japan).
To analyze the effects of the silencing of GAP-43 on BMSC differentiation, the third passage BMSCs were divided into three groups: Blank, NC (transduced with negative control lentiviral vector) and shGAP-43 (transduced with LV3-shRNA-GAP-43), which were all induced by retinal cell-conditioned differentiation medium containing 10 ng/ml brain-derived neurotrophic factor (BDNF; Sigma-Aldrich) and 5 ng/ml basic fibroblast growth factor (Sigma-Aldrich). The expression levels of GAP-43, NSE, NF, neuron-specific nuclear-binding protein (NeuN) and βIII-tubulin were detected using western blotting and cell immunofluorescence. The morphology of the BMSCs was observed under a fluorescence microscope.

ZHU Q., LIU Z., WANG C., NIE L., HE Y., ZHANG Y., LIU X, & SU G. (2015). Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats. Molecular Medicine Reports, 12(4), 5691-5700.

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Optimized 3D Constructs for Neuronal Differentiation

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Our 3D constructs for HCFs, T1DM, and T2DM cells were assembled as per our previously optimized protocol (Karamichos et al., 2009 ; Karamichos et al., 2010 (link); Karamichos et al., 2011a (link),b (link); Karamichos et al., 2012 (link)). 1 × 10⁶ cells/well were seeded on poly-carbonate membrane inserts with 0.4-μm pores (Corning Costar; Corning Incorporated, Corning, NY, USA) and cultured in EMEM containing 10% FBS and 1% antibiotic (Fig. 1). Further, the cells were treated with 0.5 μM 2-O-α-D-glucopyranosyl-L-ascorbic acid (American Custom Chemicals Corporation, San Diego, CA, USA) to stimulate ECM secretion and assembly. The cultures were maintained for 3 weeks and fresh media was supplemented every other day during the entire study period.
After 3 weeks, 8 × 10³ cells/well of SH-SY5Y human neuro-blastoma cells were seeded on top of our assembled 3D constructs and differentiation was initiated (Fig. 1). SH-SY5Ys were treated with 10 mM Retinoic Acid (Sigma Aldrich, USA) which was added to EMEM containing 1% FBS and cultured for 5 days. Cells were then switched to a treatment of serum free media of EMEM containing 2 nM of Brain-derived neurotrophic factor (BDNF) (Sigma Aldrich) and cultured for 2 additional days before they were processed for qRT-PCR and Immunofluorescence.

Priyadarsini S., Rowsey T.G., Ma J.X, & Karamichos D. (2017). Unravelling the stromal-nerve interactions in the human diabetic cornea. Experimental eye research, 164, 22-30.

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Primary Cortical Neuron Isolation and Cell Line Culturing

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Primary cortical neurons were prepared from E18.5 C57BL/6N mouse embryos, exactly as previously published (Khalfallah et al., 2018 ). CB3 cells are mouse erythroleukemia cells where the endogenous HNRNPA1 allele is inactivated due to a retroviral insertion. These cells were stably transfected to uniquely express mouse hnRNP A1 or hnRNP A1B cDNA, as previously described (Yang et al., 1994 (link)). CB3 cells were cultured in Minimum Essential Media (MEM) Alpha Modification (Fisher Scientific) supplemented with 10% FBS (Life Technologies), 1% Penicillin-Streptomycin (Wisent) and 50 mg/ml geneticin (Life Technologies) to maintain stable expression. HeLa and SH-SY5Y cells were cultured in Dulbecco’s high glucose modified Eagle medium (DMEM, Fisher Scientific) supplemented with 10% FBS and 1% L-glutamine (Sigma). For differentiation, SH-SY5Y were cultured in Dulbecco’s Modified Eagle’s Medium F-12 (DMEM F-12, Fisher Scientific) supplemented with 10% FBS, 1% L-glutamine (Sigma) and 1% Penicillin-Streptomycin (Wisent). They were differentiated with 10 μm retinoic acid (Tocris) and 50 ng/ml of Brain Derived Neurotrophic Factor (BDNF, Sigma) as published (Encinas et al., 2000 (link)).

Gagné M., Deshaies J.E., Sidibé H., Benchaar Y., Arbour D., Dubinski A., Litt G., Peyrard S., Robitaille R., Sephton C.F, & Vande Velde C. (2021). hnRNP A1B, a Splice Variant of HNRNPA1, Is Spatially and Temporally Regulated. Frontiers in Neuroscience, 15, 724307.

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