Two color flow cytometer

Manufactured by BD

The two-color flow cytometer is a laboratory instrument that analyzes and measures physical and chemical characteristics of particles, such as cells, suspended in a fluid as they pass through a beam of light. It is designed to detect and quantify two distinct fluorescent labels or markers within a sample.

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Lab products found in correlation

Annexin 5 fitc pi apoptosis detection kit, by Roche (2 mentions)

2 protocols using two color flow cytometer

Apoptosis Detection of SFN and NAC

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Cells (4 × 10⁵/well) were plated into 6-well plates. After 24 h-incubation, cells were treated with the indicated concentrations of SFN and/or NAC. After 12 h-, 24 h- and 36 h-incubation, cells were harvested, washed with PBS, and subjected to sequential staining with Annexin V-FITC/PI Apoptosis Detection Kit (Roche Applied Science) by two-color flow cytometer (BD Biosciences, NJ), according to the manufacturer's protocol. Each experiment was performed in triplicate.

Wang L., Tian Z., Yang Q., Li H., Guan H., Shi B., Hou P, & Ji M. (2015). Sulforaphane inhibits thyroid cancer cell growth and invasiveness through the reactive oxygen species-dependent pathway. Oncotarget, 6(28), 25917-25931.

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Apoptosis Analysis by Flow Cytometry

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Cells (1 × 10⁵/well) were plated into 6-well plates, and treated with the indicated concentration of trametinib. After 48 h-incubation, the cells were collected and washed with cold PBS, centrifuged and subjected to sequential staining with Annexin V-FITC/ PI Apoptosis Detection Kit (Roche Applied Science). A total of at least 10000 events were collected and analyzed by two-color flow cytometer (BD Biosciences, NJ). Each experiment was performed in triplicate.

Li M., Chen L., Qu Y., Sui F., Yang Q., Ji M., Shi B., Chen M, & Hou P. (2017). Identification of MAP kinase pathways as therapeutic targets in gallbladder carcinoma using targeted parallel sequencing. Oncotarget, 8(22), 36319-36330.

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