Cobalt acetylacetonate

MWNTs (ϕ ~ 20 nm) were kindly provided by Nikkiso Co., Ltd. PyPBI was synthesized according to a previous report26 . Nickel acetylacetonate, cobalt acetylacetonate, and a 5 wt% Nafion solution in a mixture of lower aliphatic alcohols and water were purchased from Sigma-Aldrich Co. LLC. Carbon black (XC-72R) was purchased from the Fuel Cell Store. IrCl₃·xH₂O was purchased from Tokyo Chemical Industry Co., Ltd. N,N’-dimethylacetamide (DMAc) was purchased from Kishida Chemical Co., Ltd. Ethanol, ammonia solution, 2-Propanol, and ethylene glycol (EG) were purchased from Wako Pure Chemical Industries, Ltd. Pt/C commercial powder (Pt 37 wt%) was purchased from Tanaka Kikinzoku Kogyo Co., Ltd. All the chemicals were used as received.

ROS, CAT, SOD (WST-8 method), the CCK-8 detection kit, and antioxidant NAC were purchased from Biyuntian Company (Shanghai, China). The POD detection kit, MC903, bovine serum albumin (BSA), cobalt acetylacetonate, dodecanol, octadecene, oleic acid, and oleylamine were purchased from Sigma (Shanghai, China). H₂O₂, ethanol, and isopropanol were purchased from Shenggong Company (Shenzhen, China). Rabbit anti-IgG and TSLP antibody (ab188766) were purchased from Abcam (Cambridge, UK). EDTA antigen retrieval solution, an immunohistochemistry kit, TB staining solution, and glacial acetic acid were purchased from Servicebio (Wuhan, China). Xylene and neutral gum were purchased from Sinopharm (Beijing, China). Dexamethasone (compound dexamethasone acetate gel) was purchased from Jinri Pharmaceutical Company (Xiamen, China).

The synthesis of cobalt-doped Fe₃O₄ magnetite NPs was carried out by thermal decomposition (TD), which consists of the precursor reaction at 260 °C to produce NPs. For the TD process, the following chemicals were mixed in 20 mL benzyl ether (Sigma Aldrich, St. Louis, MO, USA, 98%) solution: iron (III) acetyl acetonate (1 mmol, Sigma-Aldrich 97%), cobalt acetyl acetonate (0.5 mmol Sigma Aldrich 97%) and an organic surfactant of 0.1 M Triton X-100. Before heating, the solution was exposed to a nitrogen atmosphere (N₂) to remove oxygen and other gases from the solution. Then, the temperature was increased to 100 °C and the N₂ gas exposure was discontinued. The temperature was then increased to 260 °C and maintained for 60 min. This leads to the formation of monodisperse CoFe₂O₄ nanoparticles, which possess a characteristic dark brown color. The solution was left at room temperature for 24 h and subsequently rinsed and centrifuged for 5 cycles at 8000 RPM. In the first three cycles, 5 mL of 98% methanol was used, while in the last two cycles, 5 mL of double-distilled water was used for washing the magnetic nanoparticle solution. After centrifugation and washing, the precipitate was added into 15 mL of double-distilled water.