Mouse quadrature surface coil

Manufactured by Bruker

Sourced in Germany

The Mouse quadrature surface coil is a specialized laboratory equipment designed for magnetic resonance imaging (MRI) applications involving small animal models, such as mice. The core function of this coil is to generate and receive radiofrequency (RF) signals, which are essential for the acquisition of high-quality MRI data from small animal subjects.

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Lab products found in correlation

Fomblin, by Solvay (1 mentions) Paravision 5, by Bruker (1 mentions)

2 protocols using mouse quadrature surface coil

MRI Visualization of D-mannose(γ-Fe2O3) Nanoparticles in Mouse Brain

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To validate the MRI visibility of the analyzed D-mannose(γ-Fe₂O₃)
nanoparticles in ex vivo mouse brain, the isolated brains were washed
three times in PBS and transferred in 5 ml syringes filled with Fomblin (Solvay, Brussels,
Belgium). A custom-made holder for the syringe was placed on a mouse holder (Medres,
Cologne, Germany) and used in combination with a 9 cm resonator for transmission (Bruker,
Ettlingen, Germany) and mouse quadrature surface coil (Bruker) for signal detection. All
MR experiments were carried out on a Biospec 9.4 T animal scanner system with a 20 cm
diameter bore magnet (Bruker) operated with ParaVision 5.1 software (Bruker). Transplanted
cells were visualized performing a multi-slice multi-echo sequence using the following
parameters: TR = 4,000 ms, TE = 12 ms, slice thickness = 0.7 mm, number of slices
(coronal) = 10, FOV = 12×12 mm², matrix = 160×160, resolution 0.075×0.075
mm², bandwidth 50 kHz, echoes = 8. The acquisition time for these experiments
was 10 min and 40 s. Quantitative T2 maps were calculated using a custom-made program
developed in IDL (ITT Visual Information Solutions, Exelis Visual Information Soution,
Boulder, CO, USA). The images obtained were analyzed with the ImageJ program (NIH,
Bethesda, MD, USA).

Pongrac I.M., Radmilović M.D., Ahmed L.B., Mlinarić H., Regul J., Škokić S., Babič M., Horák D., Hoehn M, & Gajović S. (2019). D-mannose-Coating of Maghemite Nanoparticles Improved Labeling of Neural Stem Cells and Allowed Their Visualization by ex vivo MRI after Transplantation in the Mouse Brain. Cell Transplantation, 28(5), 553-567.

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Ischemic Stroke Characterization via MRI

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Experiments were performed on a horizontal 11.7 Tesla Bruker BioSpec 117/16USR system (Bruker Biospin, Ettlingen, Germany) with a 16 cm horizontal bore magnet. RF transmission was achieved with a quadrature resonator (Bruker) and the signal was detected using a mouse quadrature surface coil (Bruker For all subsequent imaging sessions the turbo spin echo sequence was coregistered to the template with the same coregistration procedure. The coregistration matrices were then used to realign the T2 maps. For reliable and objective discrimination of the ischemic territory a T2 threshold above normal was determined. For this purpose, the average T2 value of the cortex from the intact hemisphere across all animals of all age groups was calculated. The threshold was set at T2 values elevated by 10% or more above this average value. Further, a mask was created for every age group and every measurement time point to exclude the ventricles from the lesion on the T2 maps. From the coregistered T2 maps and the ischemic T2 threshold, stroke incidence maps were then created and stroke volumes were calculated.

Adamczak J., Aswendt M., Kreutzer C., Rotheneichner P., Riou A., Selt M., Beyrau A., Uhlenküken U., Diedenhofen M., Nelles M., Aigner L., Couillard-Despres S, & Hoehn M. (2017). Neurogenesis upregulation on the healthy hemisphere after stroke enhances compensation for age-dependent decrease of basal neurogenesis. Neurobiology of disease, 99.

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