Anti rhoa 26c4 mouse monoclonal antibody

Arachidonic acid (20:4,n-6), essentially fatty acid-free bovine serum albumin (BSA), and lysophosphatidic acid (LPA) (1-oleoyl) sodium salt were obtained from Sigma (St. Louis, MO, USA). 1,2-Dipalmitoyl PI and 1,2-dioleoyl PI were from Avanti Polar Lipids, Inc. (Alabaster, AL, USA).
CP55940 and Y-27632 were from Tocris (Bristol, UK). WIN55212-2 was obtained from RBI (Natick, MA, USA). O-1602 was obtained from Cayman Chemical Co. (Ann Arbor, MI, USA). PD98059, SB203580, and SP600215 were acquired from Calbiochem-Novabiochem (San Diego, CA, USA). Wortmannin was obtained from Wako Pure Chem. Ind. (Osaka, Japan). Clostridium botulinum C3 exoenzyme pcDNA4/TO and Lipofectamine^TM 2000 were from Invitrogen Life Technologies (Carlsbad, CA, USA). The anti-RhoA 26C4 mouse monoclonal antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The anti-mouse IgG horseradish-peroxidase-linked goat antibody was obtained from Medical & Biological Laboratories Co, Ltd. (Nagoya, Japan). Lipase (Rhizopus delemar) was acquired from Seikagaku Kogyo Co., Ltd. (Tokyo, Japan).

PDGF-BB was purchased form R&D System, Inc. (Minneapolis, MN, USA). GLP-1 and GIP were obtained from Peptide Institute, Inc. (Osaka, Japan). 8-Bromo cAMP, PD98059, SP600125 or SB203580 were obtained from Calbiochem-Novabiochem Co. (La Jolla, CA, USA). Dibutyryl (Bt2) cAMP was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). H89 was obtained from Seikagaku Co. (Tokyo, Japan). Phospho-specific cAMP response element-binding protein (CREB) antibodies and phospho-specific p38 MAP kinase antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies, anti-osteocalcin mouse antibody and anti-Rho-A (26C4) mouse monoclonal antibody were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Chalfront, UK). Exendin-4 was obtained from Abcam (Cambridge, UK). Other materials and chemicals were obtained from commercial sources. H89, PD98059, SP600125 or SB203580 were dissolved in dimethyl sulfoxide. The maximum concentration of dimethyl sulfoxide was 0.3%, which did not affect the cell migration assay or detection of the protein level using Western blotting^{22 (link)}.