MTS solution (Promega, USA) was used to determine whether the FD concentrations used in the experiment caused any cytotoxicity in BV2 cells. This assay is based on the mitochondrial mediated reduction of a tetrazolium compound (MTS) by living cells to form a colored formazan product which is measured colorimetrically [15 (link), 16 (link)]. Briefly, cells were seeded on a 96-well plate at the density of 5 × 104 cells/well. After 24 h, the cells were incubated with FD (0, 1, 2, 4, and 8 mg/mL) for 24 h or 48 h. 20 μL of MTS/PMS solution (Promega, USA) was added to each well. After 3 h incubation at 37°C in 5% CO2, the concentration of the MTS formazan product was measured at 490 nm (Dynex MRX II microplate reader). The average of absorbance values for triplicate wells was examined. The absorbance values of all wells were deducted with the values of DMED-treated control, which served as the background reading, and reported in percentage (%) as cell viability.
Mts pms solution
Manufactured by Promega
Sourced in United States
The MTS/PMS solution is a laboratory reagent that is used to measure the viability and proliferation of cells in culture. It provides a colorimetric method for determining the number of viable cells, which can be useful for a variety of applications such as cytotoxicity assays, cell proliferation studies, and drug screening.
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Lab products found in correlation
Celltiter 96 aqueous non radioactive cell proliferation assay mts, by Promega (3 mentions)
Mts solution, by Promega (1 mentions)
Mrx 2 microplate reader, by Dynex (1 mentions)
Human keratinocyte growth supplement, by Thermo Fisher Scientific (1 mentions)
Flat bottom 96 well plate, by Greiner (1 mentions)
Spectramax microplate reader, by Promega (1 mentions)
Elisa microplate reader, by Awareness Technology (1 mentions)
Lookout mycoplasma pcr detection kit, by Merck Group (1 mentions)
Multiskan spectrum spectrophotometer, by Thermo Fisher Scientific (1 mentions)
96 well plate, by Corning (1 mentions)
Annexin 5 binding buffer, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions)
Fluostar optima, by BMG Labtech (1 mentions)
Cd11b fitc, by BioLegend (1 mentions)
F4 80 pe, by BioLegend (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
17 protocols using mts pms solution
1
Cytotoxicity Evaluation of FD in BV2 Cells
2
Antiproliferative Assay of Compounds 10a-e
3
UVB-Induced Keratinocyte Viability Assay
4
MTS Assay for Compound Cytotoxicity
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Compound cytotoxicity was evaluated using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (MTS) following the manufacturer’s instructions (Promega, Madison, WI) using both the human breast cancer cell line MDA-MB-231 (ATCC HTB-26) and HaCaT human keratinocytes [15] (link). The HaCaT cell line was grown in Epilife medium (MEPI500Ca) with human keratinocyte growth supplement (Invitrogen, San Diego, CA). Both types of cells were harvested using Tryspin/EDTA and diluted to 1×105 cells per well in a 96-well plate. Ten concentrations covering five orders of magnitude were tested with six replicates per concentration. Cells were incubated for 48–72 hours at 37°C with 5% CO2. MTS/PMS solution (Promega, Madison, WI) was added to the wells and absorbance was measured at 490 nm two to four hours later. Data was analyzed using GraphPad Prism version 5.0 (GraphPad Software, La Jolla, CA) using a variable slope inhibition dose response curve.
5
Cell Viability Assay of CRC Cells
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One thousand CRC cells were seeded in 96-well plates (020096, Xinyou Biotech, Hangzhou, China). Cell viability was examined by incubation with MTS-PMS solution (Promega) by measuring the OD (490 nm) at the indicated time points following the manufacturer’s instructions.
6
MTS Assay for Metabolic Activity
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(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was used as an indirect colorimetric measurement of metabolic activity. Briefly, rMSCs cells were seeded in 96-well plates (1×104 cells/well) in α-DMEM supplemented with 5% FBS and antibiotics. 12 h after plating, the cells were treated with vehicle, Lv-null (10 MOI) and Lv-PSPN (10 MOI) respectively for 72 h. The medium was discarded and replaced with 100 ul of MTS/PMS solution (Promega, Madison, WI, USA) according to the manufacturer’s instructions. After incubated at 37°Cfor 1 h, an absorbance at 490 nm was measured on amicroplate reader (Bio-Rad Laboratories Inc., CA, USA). Metabolic activity was expressed as a percentage of the control cells treated with vehicle and was designated as 100%.
7
MTS Assay for Cell Viability
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Cell viability was monitored by MTS assay, a marker of mitochondrial activity, in which viable cells bioreduce the administered [3‐(4,5‐dimethyl‐thiazol‐2‐yl)‐5‐(3‐carboxymethoxy phenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium, inner salt, MTS to a coloured soluble formazan. First, SH‐SY5Y cells were seeded into plates and treated with various concentrations of neurotoxin. Next, MTS/PMS solution (Promega, USA) was added to each well and incubated for 4 hours at 37°C. Subsequently, the amount of produced formazan was ascertained at a wavelength of 490 nm using the ELISA microplate reader (Awareness, USA). The absorbance values of MPP+‐exposed cells were reported as a percentage of the untreated control cultures.
8
TBLN MNCs Proliferation Assay
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The isolated TBLN MNCs at day post challenge (DPC)-6 was subjected to cell proliferation analyses as reported previously (33 (link), 35 (link)). Briefly, 1 × 106 cells/well in triplicate in 10% FBS containing RPMI medium was plated in a 96 well flat-bottom plate (Greinerbio-one, NC). Cells were either unstimulated or stimulated with 0.1 multiplicity of infection (MOI) vaccine (H1N2-OH10) and challenge (H1N1-OH7) viruses for 72 h at 37°C in 5% CO2 incubator. The 20 μl MTS + PMS solution (Promega, WI) was added to each well before 4 h of 72 h incubation, and the OD at 490 nm was recorded using the ELISA Spectramax microplate reader. Stimulation index was calculated by dividing OD of stimulated from OD of unstimulated cells of the same animal.
9
SH-SY5Y Neurotoxicity Assay with MPP+
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In brief, SH‐SY5Y cells were seeded at a density of 2 × 104 cells/cm2 for acute and chronic MPP+ treatment. In acute MPP+ model, 24 hours after plating, the medium was replaced with the fresh medium with low serum containing 0‐3000 µM MPP+ (Sigma‐Aldrich) for 24 hours. To create chronic MPP+ model, the cells were exposed to a low‐serum fresh medium containing the same concentrations of MPP+ 3 times per week for up to 2 weeks. Cell viability was assessed using MTS/PMS (phenazine methosulphate). Following neurotoxin treatment, 20 µL of MTS/PMS solution (Promega) was added to each well of 96‐well plates and incubated for 4 hours at 37°C in a 5% CO2 atmosphere. The wells were then evaluated for colorimetric absorption with the ELISA microplate reader (Awareness Technology Inc) at a wavelength of 490 nm.
10
Cell Proliferation and Viability Assay
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