Kh2po4

Manufactured by Beijing Chemical Works

Sourced in China

KH2PO4 is a chemical compound commonly used as a laboratory reagent. It is a colorless, crystalline solid that is soluble in water. KH2PO4 is primarily used as a buffer solution in biochemical and biological applications to maintain a specific pH range.

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Lab products found in correlation

6 protocols using kh2po4

Analytical Reagents and Protein Expression

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Copper sulphate (CuSO₄), sodium hydroxide (NaOH), hydrochloric acid (HCl), NaCl, KCl, Na₂HPO₄·12H₂O, KBr and KH₂PO₄ were bought from Beijing Chemical Factory (China). Dithiothreitol (DTT), quinine sulfate and amino acids were provided by Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). Chlortetracycline HCl (CTC), tetracycline HCl (TC), oxytetracycline HCl (OTC) and doxycycline HCl (DC) were purchased from Macklin Reagent Co., Ltd. (Shanghai, China). The GST protein was expressed according to our previously reported method.^{27 (link)} All chemicals were of analytical quality without additional purification.

Wang J., Chen W., Cao L., Zhou M., Geng Y., Liu Y., Ding S, & Fu D.Y. (2023). Glutathione S-transferase templated copper nanoclusters as a fluorescent probe for turn-on sensing of chlorotetracycline. Nanoscale Advances, 6(2), 722-731.

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Isolation and Characterization of Heterophyllins

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Heterophyllin A (1) and Heterophyllin B (2) (Figure 7) were provided by Professor Ninghua Tan (Kunming institute of botany, Chinese academy of science, Kunming, China). HPLC analysis showed that their purities were over 98%. Analytical grade methanol and acetonitrile were purchased from Sigma (St. Louis, MO, USA). D₂O was bought from Norell (Landisville, NJ, USA). Sodium 3-trimethlysilyl [2, 2, 3, 3-d₄] propionate (TSP) was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA). KH₂PO₄ was from Beijing Chemical Works (Beijing, China), and NaOD was purchased from Armar (Dottingen, Switzerland).

Hua Y., Hou Y., Wang S., Ma Y., Liu Z., Zou L., Liu X., Luo Y, & Liu J. (2016). Comparison of Chemical Compositions in Pseudostellariae Radix from Different Cultivated Fields and Germplasms by NMR-Based Metabolomics. Molecules, 21(11), 1538.

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NMR Analysis of Tissue Extracts

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Methanol, Na₂HPO₄ and KH₂PO₄ were purchased as analytical grade from Beijing Chemical Works (Beijing, China). Sodium 3-trimethlysilyl [2,2,3,3-d₄] propionate (TSP) was from Cambridge Isotope Laboratories Inc. (Andover, MA, USA). D₂O was obtained from Norell (Landisville, Pennsylvania, USA). Phosphate buffer, prepared by dissolving KH₂PO₄ and Na₂HPO₄ in water (0.2 M, pH 7.4) containing 0.01% TSP and 10% D₂O, was used to reconstructed tissue extract for NMR analysis. Bull Serum Albumin (BSA), primary antibodies, including rabbit antibodies to HGF (Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit antibodies to TGF-β1 (Santa Cruz Biotechnology), secondary antibody: polymerized HRP, 3,3′-diaminobenzidine (DAB), Eukitt mounting medium (Sigma-Aldrich), Tris Buffered Saline (TBS), and phosphate buffer (PBS) were bought from BosterBio Co., Ltd (Wuhan, China).

Li Z., Li A., Gao J., Li H, & Qin X. (2016). Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR. Frontiers in Pharmacology, 7, 307.

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Measuring Neurotransmitters in Freely Moving Animals

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3,3′-Iminodipropionitrile (IDPN) was purchased from Sigma-Aldrich Co. (St Louis, MO, USA), and tiapride (Tia) from Jiangsu Nhwa Pharmaceutical Co., Ltd. (Xuzhou, People’s Republic of China). o-Phthalaldehyde, Glu, GABA, sodium heptane sulfonate, sodium acetate, boric acid, and β-mercaptoethanol were obtained from Sigma-Aldrich Co. High-performance liquid chromatography (HPLC)-grade methyl alcohol was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Ethylene diamine tetraacetic acid and KH₂PO₄ were obtained from Beijing Chemical Works (Beijing, People’s Republic of China).
The microdialysis system consisted of a CMA/120 system for freely moving animals (table, cage, and balancing arm) together with a CMA/402 microdialysis pump, a 5 mL glass syringe, a CMA/12 microdialysis probe, and a CMA/470 refrigerated fraction collector.

Zhang W., Wei L., Yu W., Cui X., Liu X., Wang Q, & Wang S. (2016). Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome. Neuropsychiatric Disease and Treatment, 12, 1233-1242.

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Electrochemical Biosensing with GO-AuNPs

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All chemicals used were analytical grade and without further purification. GO sheets were purchased from Nanjing XFNANO Materials Tech Co., Ltd. KH₂PO₄ (99.5%), KCl (99%), Na₂HPO₄ (99%), NaCl (99.5%) and sodium citrate (C₆H₅Na₃O₇·2H₂O, 99%) were purchased from Beijing Chemical Works. HAuCl₄·3H₂O (99.9%), Chitosan (93%), AA (99%), DA (99%), UA (99%) and NADH (97%) were supplied by Sigma-Aldrich. PBS (0.1 M, pH 7.4) was prepared by dispersing 0.2 g KCl, 8 g NaCl, 0.2 g KH₂PO₄ and 1.54 g Na₂HPO₄ in 1 L deionized water.

Song J., Xu L., Xing R., Li Q., Zhou C., Liu D, & Song H. (2014). Synthesis of Au/Graphene Oxide Composites for Selective and Sensitive Electrochemical Detection of Ascorbic Acid. Scientific Reports, 4, 7515.

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Irisensata Thunb Grass Cultivation and Utilization

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Irisensata Thunb grass (ITG) was obtained from the experimental area of Jilin Agricultural University (Changchun, China) in 2022, washed with deionized water, dried at 80 °C for 12 h, and crushed. Aspergillus niger (A. niger) was supported by the Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, Ministry of Education.
D-glucose monohydrate and agar were provided by Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). Potatoes were bought from a local supermarket. (NH₄)₂SO₄, KH₂PO₄, MgSO₄, CaCl₂, NaCl, FeSO₄, MnSO₄, ZnCl₂, NaOH, HCl, and KOH were purchased from Beijing Chemical Works (Beijing, China) and used without further purification. Mixed base (MB) was prepared by using equal masses of NaOH and KOH.
Rhodamine B (RhB, CAS: 81−88−9) and Tetracycline hydrochloride (TH, CAS: 64−75−5) were supplied by Aladdin Chemical (Shanghai) Co. Ltd. (Shanghai, China) and the structural formulas of RhB and TH are shown as Figure S1 (Supplementary Material).

Su Y., Xie K., Xiao J, & Chen S. (2022). Influence of Microbial Treatment on the Preparation of Porous Biochar with Stepped-Up Performance and Its Application in Organic Pollutants Control. International Journal of Molecular Sciences, 23(22), 14082.

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