To determine the depth of microchannels created by microneedles, CLSM studies were carried out (n=3). Fluoresoft® (0.35%, 200 μl) was applied to full-thickness human skin treated with the microneedles for 1 min. Excess calcein was removed, and the skin sample was placed on the glass slide and visualized using a computerized Leica SP8 confocal laser microscope. Samples were viewed with a 10X objective at an excitation wavelength of 496 nm. X-Z sectioning was employed using Application Suite-Advanced Fluorescent software (step size = 15 μm) by Leica to determine the depth of microchannels.
Application suite advanced fluorescent software
Manufactured by Leica
The Application Suite-Advanced Fluorescent software is a comprehensive imaging and analysis tool designed for Leica's fluorescence microscopy systems. It provides advanced functionality for visualizing, processing, and analyzing fluorescent samples. The software supports a range of features, including image acquisition, multi-dimensional data management, and quantitative analysis.
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Lab products found in correlation
2 protocols using application suite advanced fluorescent software
1
Microchannel Depth Determination by CLSM
2
Visualizing Microneedle-Created Microchannels
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To determine the depth of microchannels created by microneedles, CLSM studies were carried out (n=3). Fluoresoft® (0.35%, 200 μl) was applied to full-thickness human skin treated with the microneedles for 1 min. Excess calcein was removed, and the skin sample was placed on the glass slide and visualized using a computerized Leica SP8 confocal laser microscope. Samples were viewed with a 10X objective at an excitation wavelength of 496 nm. X-Z sectioning was employed using Application Suite-Advanced Fluorescent software (step size = 15 μm) by Leica to determine the depth of microchannels.
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