We carried out 10% polyacrylamide gel electrophoresis, for which 30 μg of the specimens were loaded in each well. Following that, the proteins were transferred onto PVDF membranes (EMD Millipore, Billerica, MA, USA). The membranes were blocked with 5% skim milk for 1 hour at room temperature before incubation with primary antibodies (rabbit anti-MMP-2 antibody or rabbit anti-MMP-9 antibody, dilution: 1:300, Santa Cruz; rabbit anti-PDGFR-α antibody or rabbit anti-PDGFR-β antibody, dilution: 1:50, Santa Cruz; rabbit anti-β-actin antibody, dilution: 1:1000, KeyGEN BioTECH Jiangsu, P.R. China) for 3 hours at room temperature. Horseradish peroxidase-labeled anti-rabbit or anti-goat IgG were used as secondary antibodies (dilution: 1:2000). After DAB staining, the membrane was placed in a gel imager for scanning. Quantity One software was used for quantitation. The following equation was used to calculate the relative expression of proteins: relative protein expression = target protein intensity/internal control (β-actin) intensity.
Rabbit anti pdgfr α antibody
Manufactured by Santa Cruz Biotechnology
Sourced in China, United States
The Rabbit anti-PDGFR-α antibody is a laboratory reagent used to detect and study the Platelet-Derived Growth Factor Receptor Alpha (PDGFR-α) protein in various biological samples. It is a polyclonal antibody raised in rabbits and specifically targets the PDGFR-α protein.
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2 protocols using rabbit anti pdgfr α antibody
1
Western Blot Analysis of MMPs and PDGFRs
2
Characterization of GD3 and GD2 Antibodies
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Mouse anti‐GD3 mAb (R‐24) was obtained from Dr. L.J. Old (Memorial Sloan Kettering Cancer Center, New York, NY, USA). Mouse anti‐GD2 mAb (220‐51) was generated in our laboratory. Rabbit anti‐flotillin‐1 antibody, rabbit anti‐caveolin‐1 antibody, mouse anti‐CD109 mAb, rabbit anti‐PDGFRα antibody, goat anti‐ASCT2 antibody, and mouse anti‐c‐myc antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti‐ASCT2 antibody, rabbit anti‐phospho‐mTOR antibody (Ser2448), rabbit anti‐mTOR mAb, mouse anti‐phospho‐p70 S6 kinase mAb (Thr389), rabbit anti‐p70 S6 kinase mAb, rabbit anti‐S6 ribosomal protein mAb, rabbit anti‐phospho‐S6 ribosomal protein mAb (Ser235/Ser236), rabbit anti‐EphA2 mAb, and rabbit anti‐CD44 antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Goat anti‐urokinase plasminogen activator surface receptor antibody was purchased from R&D Systems (Minneapolis, MN, USA). Rabbit anti‐c‐myc antibody was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Rabbit anti‐ASCT2 conjugated with Alexa 488 was purchased from Bioss (Woburn, MA, USA).
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