Cell proliferation ability was detected by the CCK8 assay (Sangon Biotech Co., Ltd., China). At the end of transfection, cells were plated in 96-well plates (5 × 103 cells/well) and then cultured at 37°C. Next, CCK8 solution was added according to the manufacturer's instructions at 24, 48, 72, 96, and 120 h, respectively. The cells were then cultured for another 1.5 h in an incubator (37°C). The optical density value at 450 nm (OD450) was detected.
Cck 8 assay
Manufactured by Sangon
Sourced in China
The CCK-8 assay is a colorimetric method used to measure the metabolic activity of cells. It utilizes the tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] to produce a water-soluble formazan dye upon reduction by dehydrogenases in viable cells. The amount of formazan dye generated is directly proportional to the number of living cells, providing a quantitative assessment of cell proliferation, cytotoxicity, and viability.
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Lab products found in correlation
6 protocols using cck 8 assay
1
Cell Proliferation Assay Protocol
2
Cell Proliferation Quantification by CCK-8
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For cell proliferation detection, we used CCK-8 assay according to the manufacturer's recommendations (Sangon Biotech Co., Ltd., Shanghai, China). Infected or transfected cells were cultured in a 96-well plate at a density of 2,000 cells/well in quintuplicate. CCK-8 solution (10 µl) was added to each well plate and was incubated for 4 h in an incubator. Cells were incubated at 37°C for 4 h. The absorbance at 450 nm was measured by a microplate reader.
3
Evaluating CuSO4 Toxicity on pGCs
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To assess the toxicity of different concentrations of CuSO4 on pGCs, the cell viability of pGCs treated with different concentrations of CuSO4 was measured by CCK8 assay (Sangon Biotech, Shanghai, China). Cells were counted and seeded into 96-well plates at a density of 5000 cells/well and grown for 24 h. After treatment with drugs for the indicated time, the original medium was removed and 110 µL DMEM/F12 containing 10 µL CCK8 was added. After incubation in an incubator containing 5% CO2 at 37 °C for 2 h, the absorbance value at 450 nm was measured using a microplate reader. All reactions were repeated six times (n = 6).
4
Cytotoxicity Evaluation of Drugs on OSCC Cells
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CCK-8 assay (Sangon, Shanghai, China) was used to detect the cytotoxic effect of different drug treatments on cancer cells and the 50% inhibitory concentration (IC50) of the drug was calculated. OSCC cells were plated into 96-well plates with a density of 5 × 103 cells/well, supplied with 100 µL complete growth medium. After 24 h incubation, cells were exposed to CoCl2, YC-1, and 3-MA at the concentrations of 25 μM to 200 μM, 10 μM to 100 μM, 0.5 μM to 150 μM, respectively. Untreated cells were used as control. At each time point, the cells were washed and incubated with 100ul RPMI 1640 plus 10 μL CCK-8 solution at 37 °C for 3 h. Subsequently, the absorbance was measured at 450 nm with a microplate reader (Bio Tek Instruments, Inc., USA). Each experiment was performed at least in triplicate. Dose-response curves were established to determine the IC50 values for CoCl2, YC-1, and 3-MA in the two OSCC cell lines.
5
CCK-8 Assay for Cell Viability
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Cell viability was measured using a CCK-8 assay (#E606335, Sangon Biotech, Shanghai, China) following the protocol. HTMCs were cultured and treated with different conditions and then plated in 96‐well plates with a density of 2 × 104 cells/well for 24, 48, and 72 h. Then, the medium was changed with 100 μL of fresh medium, and 10 μL of CCK-8 reagent was added per well. After incubation for 1 h, absorbance at 450 nm was detected using a Varioskan Flash multimode reader (Thermo Fisher Scientific).
6
Cytocompatibility of UIHA Biomaterial
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The cytocompatibility of UIHA at different weight fractions was examined by using endothelial cells and a live/dead assay. In brief, endothelial cells were seeded and cultured on the surface of the UIHA for 24 hours at 37°C and 5% CO2. Cell viability test was performed with a live/dead viability/cytotoxicity kit for mammalian cells. An inverted fluorescence microscope (EVOS FL Auto, Life Technologies) was applied to image live (green stain) and dead (red stain) cells. ImageJ software was used to calculate the cell viability by dividing the number of live cells by the total number of cells. CCK-8 assay (Sangon Biotech) test was also carried out to quantify the cell viability in accordance with the instruction provided by the manufacturer.
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