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N butanol

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N-butanol is a colorless, flammable liquid chemical compound with the formula CH3(CH2)3OH. It is a primary alcohol with a four-carbon structure. N-butanol is commonly used as a solvent and as an intermediate in chemical synthesis.

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Lab products found in correlation

Methanol, by Thermo Fisher Scientific (9 mentions) Acetonitrile, by Thermo Fisher Scientific (8 mentions) Ethanol, by Thermo Fisher Scientific (5 mentions) Chloroform, by Thermo Fisher Scientific (4 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (4 mentions) N hexane, by Thermo Fisher Scientific (4 mentions) Dichloromethane, by Thermo Fisher Scientific (3 mentions) Ethyl acetate, by Thermo Fisher Scientific (3 mentions) Hplc grade ethanol, by Thermo Fisher Scientific (2 mentions) Milli q system, by Merck Group (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) Methanol d4, by Merck Group (2 mentions) Formic acid, by Biosolve (2 mentions) Acetonitrile, by Biosolve (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Hplc grade chloroform, by Thermo Fisher Scientific (1 mentions) Perchloric acid, by Merck Group (1 mentions) Hexane, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Ammonium molybdate, by Thermo Fisher Scientific (1 mentions)

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Butanol (16 citations)

20 protocols using n butanol

1

Synthetic Lipid Standards for Research

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Synthetic lipid standards [PC 12:0/12:0 (850335), PE 17:0/14:1 (LM-1104), PI 17:0/14:1 (LM-1504), PS 17:0/14:1 (LM-1304), Cer d18:1/17:0 (860517), SM d18:1/12:0 (860583), HexCer d18:1/8:0 (860540), DoxDHCer m18:0/12:0 (860460), 17:0-17:1-17:0 D5 TG (860903), 1,3-17:0 D5 DG (800854) and N-C12-deoxysphingosine m18:1/12:0 (860455)] were from Avanti Polar Lipids Inc. MTBE (methyl-tert-butyl ether) and methylamine (33% in absolute ethanol) were purchased from Sigma Aldrich. Chloroform, methanol, n-butanol and ammonium molybdate were from Acros Organics. LC-MS grade methanol, water and ammonium acetate were from Fluka. HPLC-grade Chloroform was purchased from Acros Organics. Monopotassium phosphate, L-ascorbic acid, 70% perchloric acid, hexane, methyl acetate and acetonitrile were from Merck.
Sinturel F., Chera S., Brulhart-Meynet M.C., Montoya J.P., Stenvers D.J., Bisschop P.H., Kalsbeek A., Guessous I., Jornayvaz F.R., Philippe J., Brown S.A., D’Angelo G., Riezman H, & Dibner C. (2023). Circadian organization of lipid landscape is perturbed in type 2 diabetic patients. Cell Reports Medicine, 4(12), 101299.
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2

Lipidomic Analysis of Cellular Samples

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LC-MS grade water was provided by Huberlab; methanol, acetonitrile (both HPLC grade) by VWR; ammonium acetate by Sigma-Aldrich; acetic acid by Biosolve; n-butanol by Acros Organics (Thermo Fisher Scientific); 40% methylamine solution in water by Sigma-Aldirch. Splash Lipidomix and 18:0 Cer(d7) were purchased from Avanti Polar Lipids.
Nyonda M.A., Kloehn J., Sosnowski P., Krishnan A., Lentini G., Maco B., Marq J.B., Hannich J.T., Hopfgartner G, & Soldati-Favre D. (2022). Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii. Cell Reports, 40(7), 111224.
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3

Ginsenoside Profiling by HPLC

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High-performance liquid chromatography (HPLC)-grade ethanol, n-butanol, acetonitrile, and dimethyl sulfoxide were purchased from Fisher Scientific (Pittsburgh, PA, USA). Milli-Q water was supplied by a water purification system (US Filter, Palm Desert, CA, USA). Standards of ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Re, Rf, Rg1, Rg2, 20R-Rg2, Rg3, Rh1, and Rh2 were obtained from INDOFINE Chemical Company (Somerville, NJ, USA) or Delta Information Center for Natural Organic Compounds (Xuancheng, Anhui, China). All standards were of biochemical-reagent grade and at least 95% pure. Other reagents were of biochemical-reagent grade.
Yu C., Wen X.D., Zhang Z., Zhang C.F., Wu X., He X., Liao Y., Wu N., Wang C.Z., Du W., He T.C, & Yuan C.S. (2015). American ginseng significantly reduced the progression of high-fat-diet-enhanced colon carcinogenesis in ApcMin/+mice. Journal of Ginseng Research, 39(3), 230-237.
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4

Antioxidant and Cytotoxicity Assays

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Methanol, n-hexane, n-butanol, ethyl acetate, chloroform, Triton X-100 and Tween 20 were purchased from Fisher Scientific (Loughborough, Leicestershire, UK). Linoleic acid, gallic acid, quercetin, β-carotene (Type I synthetic, 95%), bovine serum albumin (BSA), ABTS, potassium persulfate, ferrous sulfate, hydrogen peroxide, AAPH, tetramethylchroman-2-carboxylic acid (trolox), ethylenediamine tetra acetic acid (EDTA), DPPH, dichloro-dihydro-fluorescein diacetate (DCFH-DA), Folin–Ciocalteu’s phenol reagent and ferrozine were supplied by Sigma-Aldrich Co. (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), gentamicin and fetal bovine serum (FBS) were purchased from GIBCO (Grand Island, NY, USA). The 8-iso prostaglandin F2α ELISA kit was bought from Cusabio (Wuhan, China). MTT and neutral red were obtained from Nacalai Tesque (Kyoto, Japan). All of the other chemicals were purchased from Sigma-Aldrich Co.
Ooi D.J., Chan K.W., Sarega N., Alitheen N.B., Ithnin H, & Ismail M. (2016). Bioprospecting the Curculigoside-Cinnamic Acid-Rich Fraction from Molineria latifolia Rhizome as a Potential Antioxidant Therapeutic Agent. Molecules, 21(6), 682.
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5

Ginsenoside Standards for Cell Proliferation Assay

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Ginsenoside standards used in this study, purchased from Delta Information Center for Natural Organic Compounds (Xuancheng, AH, China), were of biochemical-reagent grade and were at least 98% pure, as confirmed by HPLC. 5-FU was obtained from American Pharmaceutical Partners Inc. (Schaumburg, IL, USA). HPLC grade ethanol, n-butanol and acetonitrile were obtained from Fisher Scientific (Pittsburgh, PA, USA). Milli-Q water was supplied by a water purification system (US Filter, Palm Desert, CA, USA). Trypsin, McCoy’s 5A medium, fetal bovine serum (FBS), penicillin/streptomycin solution (200×), propidium iodide (PI) and RNase were obtained from Mediatech, Inc. (Herndon, VA, USA). A CellTiter 96 Aqueous One Solution cell proliferation assay kit was obtained from Promega (Madison, WI, USA). Annexin V- fluorescein isothiocyanate (FITC) and cyclin A-FITC were obtained from BD Biosciences (San Diego, CA, USA).
Wan J.Y., Yao H., Zhang C.F., Huang W.H., Zhang Q., Liu Z., Bi Y., Williams S., Wang C.Z, & Yuan C.S. (2018). Red American ginseng enhances the effect of fluorouracil on human colon cancer cells via both paraptosis and apoptosis pathways. Journal of applied biomedicine, 16(4), 311-319.
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6

Standardized Ginsenoside Analysis Protocol

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Standards of ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1, Rg2, 20(R)-Rg2, Rg3, and Rh1 were obtained from Indofine Chemical Company (Somerville, NJ, USA) and Delta Information Center for Natural Organic Compounds (Xuancheng, AH, China). All standards were of biochemical-reagent grade and at least 95% pure. AOM was obtained from the NCI Chemical Carcinogen Reference Standard Repository, Midwest Research (Kansas City, MO, USA). DSS (molecular weight of 36–50 kDa) was obtained from MP Biomedicals (Solon, OH, USA). HPLC grade ethanol, n-butanol, acetonitrile, and dimethylsulfoxide were obtained from Fisher Scientific (Pittsburgh, PA, USA). Milli Q water was supplied by a water purification system (US Filter, Palm Desert, CA, USA). Hemoccult Sensa test strips were obtained from Beckman Coulter (Brea, CA, USA). Multi-Analyte ELISArray Kits for inflammatory cytokine analysis were obtained from Qiagen (Germantown, MD, USA).
Yu C., Wen X.D., Zhang Z., Zhang C.F., Wu X.H., Martin A., Du W., He T.C., Wang C.Z, & Yuan C.S. (2014). American ginseng attenuates azoxymethane/dextran sodium sulfate-induced colon carcinogenesis in mice. Journal of Ginseng Research, 39(1), 14-21.
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7

Isolation and Characterization of Ginsenosides

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Ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Rg1, Rg2, Re and Rh1 were obtained from Delta Information Center for Natural Organic Compounds (Xuancheng, AH, China). HPLC grade methanol, n-butanol, acetonitrile, and absolute ethanol were obtained from Fisher Scientific (Pittsburgh, PA). Mili Q water was supplied by a water purification system (US Filter, Palm Desert, CA).
Wang C.Z., Huang W.H., Zhang C.F., Wan J.Y., Wang Y., Yu C., Williams S., He T.C., Du W., Musch M.W., Chang E.B, & Yuan C.S. (2017). Role of intestinal microbiome in American ginseng-mediated colon cancer protection in high fat diet-fed AOM/DSS mice. Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 20(3), 302-312.
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8

Solvent and Reagent Acquisition for Analytical Chemistry

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Dichloromethane, ethyl acetate, n-butanol, n-hexane, acetone, acetonitrile, acetonitrile far UV and methanol (all HPLC grade) were purchased from Fisher Scientific (Leicestershire, UK).
All reagents, such as sulphuric acid and formic acid (eluent additive for HPLC) were purchased from Acros Organics (Geel, Belgium) or Sigma-Aldrich (St. Louis, MO, USA). acetonitrile and formic acid (both for UPLC-MS) were obtained from Biosolve Chimie (Dieuze, France).
Ultrapure water was obtained using a Milli-Q system from Millipore (Bedford, MA, USA).
NMR deuterated solvents (CDCl3, acetonitrile-d3, methanol-d4, DMSO-d6, pyridine-d5) were obtained from Sigma-Aldrich.
Baldé M.A., Tuenter E., Matheeussen A., Traoré M.S., Cos P., Maes L., Camara A., Diallo M.S.T., Baldé E.S., Balde A.M., Pieters L, & Foubert K. (2021). Bioassay-guided isolation of antiplasmodial and antimicrobial constituents from the roots of Terminalia albida. Journal of ethnopharmacology, 267.
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9

Inflammation Biomarker Analysis Protocol

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Standard geraniin and azoxymethane were purchased from Sigma Aldrich (St. Louis, USA) . HPLC grade n-butanol, ethanol, dimethylsulfoxide, and acetonitrile were purchased from Fisher Scientific (Pittsburgh, PA, USA) . Multi-Analyte ELISA kits for inflammatory mediators and proinflammatory cytokines were obtained from Qiagen (Germantown, MD, USA) . All the reagents used in the experimental protocol were analytical grade and 95％ pure.
Zhou R., Lei J., Wei Y, & Zhang H. (2021). Chemoprotective Effects of Geraniin against Azoxymethane Induced Colorectal Cancer by Reduction of Inflammatory Reaction. Journal of oleo science, 70(6).
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10

Pharmaceutical Compound Characterization Protocol

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• TR was bought from Xiamen Keerda Bio-Tech Co., Ltd (Fujian, China), with a purity of 98%.
• AC was supplied from Sigma-Aldrich, Germany.
• Organic solvents (HPLC grade) such as methanol, ethanol, propanol, and n-butanol were purchased from Fisher Scientific UK, Loughborough Leics (UK).
• Different surfactants including carboxy methylcellulose (CMC), sodium dodecyl sulphate (SDS), Tween 80, and cetrimide were bought from El-Nasr Pharmaceutical Chemicals Co., Abu Zaabal, Egypt.
• Talc, calcium stearate, crystalline cellulose, and maize starch were provided from El-Nasr Pharmaceutical Chemicals Co., Abu Zaabal, Egypt.
• Acetic acid, boric acid, phosphoric acid, and sodium hydroxide were used for the preparation of the Britton–Robinson (BR) buffer and were all supplied from Sigma-Aldrich, Germany.
Dagher D., Elmansi H., Nasr J.J, & El-Enany N. (2022). Utility of a novel turn-off fluorescence probe for the determination of tranilast, an adjunctive drug for patients with severe COVID-19. RSC Advances, 12(34), 22044-22053.
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