Label-free proteomic analysis was performed in a nanoACQUITY UPLC system (Waters, Milford, MA) coupled to a Xevo Q-TOF G2 mass spectrometer (Waters, Milford, MA), as described elsewhere (Lobo, Leite et al. 2015). The nanoACQUITY UPLC system is equipped with a Trap Columm (100Å, 5 µm, 180 µm × 200 mm) and a HSS T3 M-Class type column (analytical column 75 μm × 150 mm; 1.8 μm) (Waters, Milford, MA). ProteinLynx GlobalServer software (PLGS) version 3.03 (Waters, Milford, MA) was used to process and search the LC-MSE continuum data.
Peptides identification and difference in expression among the groups was obtained using the Protein Lynx Global Server (PLGS) software (version 3.03, Waters Co., UK) as described elsewhere (Lima Leite, Gualiume Vaz Madureira Lobo et al. 2014 (link)). The procedures and bioinformatics analysis were performed as described previously (Dionizio, Melo et al. 2018, Dionizio, Melo et al. 2020 ).
Peptides identification and difference in expression among the groups was obtained using the Protein Lynx Global Server (PLGS) software (version 3.03, Waters Co., UK) as described elsewhere (Lima Leite, Gualiume Vaz Madureira Lobo et al. 2014 (link)). The procedures and bioinformatics analysis were performed as described previously (Dionizio, Melo et al. 2018, Dionizio, Melo et al. 2020 ).