HL-1 cells were purchased from Sigma-Aldrich (SCC065). HL-1 cells were cultured with Calycomb Medium (Sigma, United States) supplemented with 10% fetal bovine serum (FBS), 1% Penicillin/Streptomycin, 2 mM L-Glutamine, and 0.1 mM Norepinephrine. The cells were cultured in flasks coated with 5 μg/ml fibronectin and 0.02% gelatin at 37°C in a 5% CO2 atmosphere. Then the cells were cultured in six-well dishes to induce tachypacing using a cell pacing system (Ionoptix, USA). The cells were subjected to rapid field stimulation for 24 h at 7 Hz (20 V, 5 ms). In a few experiments, HL-1 cells were pre-treated with 20 nM Fgf21 for 24 h before rapid field stimulation.
Calycomb medium
Manufactured by Merck Group
Sourced in United States
Calycomb Medium is a laboratory product used for the cultivation and growth of microorganisms. It serves as a nutrient-rich culture medium that supports the development of various types of microbes in a controlled environment.
Automatically generated - may contain errors
2 protocols using calycomb medium
1
Tachypacing-Induced Cardiac Hypertrophy in HL-1 Cells
2
Cell Culture Conditions for Diverse Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The lung cancer cell line (A549), normal intestinal epithelial cell line (FHs 74 Int), normal liver epithelial cell line (THLE-2), and cardiac muscle cells (HL-1) were used in this research. A549 cells were cultured in RPMI-1640 medium supplemented with 10 % fetal bovine serum (FBS) (Gibco), 1 % penicillin, and streptomycin (Sigma-Aldrich). FHs 74 Int and THLE-2 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Sigma-Aldrich) supplemented with 10 % FBS, 1 % penicillin, and streptomycin. HL-1 cells were cultured in the Calycomb Medium (Sigma-Aldrich) supplemented with 10 % F2442 serum, 1 % Norepinephrine, 1 % l -glutamine, and 1 % penicillin and streptomycin. All four kinds of the cell were cultured at 37 °C in a humidified atmosphere containing 5 % CO2. Cells would become mature in 3–5 days, and the medium was changed every 48 h.
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