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Mp e 65 lens

Manufactured by Canon
Sourced in Japan

The Canon MP-E 65mm f/2.8 1-5x Macro Photo lens is a specialized lens designed for close-up photography. It provides a magnification range of 1x to 5x, allowing users to capture detailed, life-size images of small subjects. The lens features a fixed focal length of 65mm and a maximum aperture of f/2.8, enabling shallow depth of field and excellent image quality.

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2 protocols using mp e 65 lens

1

Detailed Protocolo for Xanthopygina Taxonomy

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Photographs were taken using a Visionary Digital Passport System with a Canon EOS 40D camera and MP-E 65 lens. Images were automontaged using Helicon Focus 6.2.2. SEM photographs were taken using a Neoscope JEOL desktop SEM and processed using the Fluid Mask 3 software. All specimens were examined using an Olympus SZX10 stereomicroscope. Measurements were made using an ocular micrometer. Width: length ratio measurements were made on the widest and longest parts of the structure. The comparison between the length of the median lobe and the paramere excludes the bulbous basal part of the median lobe. Total body length is measured from the anterior margin of frons to the posterior margin of tergite VIII. Terminology and label data follow the procedure established by Ashe and Chatzimanolis 2003 and used in other Xanthopygina taxonomic works (e.g., Chatzimanolis 2004 , Chatzimanolis 2008 (link), Chatzimanolis 2012 (link), Chatzimanolis 2013 (link), Chatzimanolis 2014a (link), Chatzimanolis and Ashe 2009 ). The type locality of Scaponopselaphusmutator (Sharp) has been corrected as indicated in Asenjo et al. 2013 .
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2

Multi-Modal Imaging of Insect Specimens

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Face, lateral and dorsal habitus, and metasoma images of all included species were generated with a Canon EOS 7D camera with MPE-65 lens (Canon, Ōta, Tokyo, Japan). Three wireless flash units were arranged at approximately 120 degree intervals around the specimen. A tube of diffusion paper was placed between the flash units and the specimen to provide a uniform, bright, diffuse light source, as for microscope examination. The camera was mounted on a StackShot system (Cognisys Inc., Traverse City, Michigan, United States) to obtain several images at varying focal depths, which were then processed for focus stacking in Helicon Focus software (Helicon Soft Ltd, Kharkiv, Ukraine). Male genitalia and some diagnostic characters were illustrated in Illustrator CS5 software (Adobe Inc., San Jose, California, United States), while examining the character using a microscope with ocular grid for scale reference.
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