Anti mouse cd73

Manufactured by BioLegend

Anti-mouse CD73 is a lab equipment product that specifically binds to the CD73 protein expressed on the surface of mouse cells. CD73 is an enzyme involved in the regulation of extracellular adenosine levels. The core function of this product is to detect and quantify the expression of CD73 in mouse samples.

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Lab products found in correlation

Anti mouse sca1, by BioLegend (1 mentions) Anti mouse igg peroxidase a4416, by Merck Group (1 mentions) Mouse anti tg2 antibody, by Thermo Fisher Scientific (1 mentions) Anti mouse cd106, by BioLegend (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions) Anti mouse cd45, by BioLegend (1 mentions) Antimousecd29, by BioLegend (1 mentions) Antirabbit igg peroxidase a0545, by Merck Group (1 mentions) Anti mouse cd146, by BioLegend (1 mentions) Pab419 sc 58665, by Santa Cruz Biotechnology (1 mentions) Perilipin, by Cell Signaling Technology (1 mentions) Facscalibur, by BD (1 mentions)

Spelling variants of this product

Anti-CD73 (23 citations) PE anti-mouse CD73 (2 citations) PE-conjugated anti-mouse CD73 (Cat No: 127205) (2 citations)

3 protocols using anti mouse cd73

Spheroid and Subspheroid Characterization Assay

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For spheroid and subspheroid assays, growth
supplements were used: B27 supplement (50×) (Gibco, 15504-044),
fibroblast-growth factor (100 μg/mL) (Gibco, AA-10-155), insulin
transferrin selenium (ITS) (Gibco, 41400-045), N2 supplement (100×)
(Gibco, 17502-048), and epithelial-growth factor (EGF) (200 μg/mL)
(Invitrogen, PHG0313); mouse-anti-TG2 antibody (CUB 7402, Thermo Fisher
Scientific, M S-224-P), monoclonal mouse anti-ITGβ1 antibody
(Santa Cruz, sc-8978), and anti-SDC-4 antibody (Abcam, ab24511). For
characterization, antimouse CD11b (BioLegend, B192967), antimouse
Sca-1 (BioLegend, B163257), antimouse CD44 (BioLegend, B193068), antimouse
CD45 (BioLegend, B187805), antimouse CD73 (BioLegend, B182619), antimouse
CD29 (BioLegend, B181560), and antimouse CD106 (BioLegend, B178443).
Antirabbit IgG peroxidase (A0545) and antimouse IgG peroxidase (A4416)
conjugates were purchased from Sigma.

Ulukan B., Bihorac A., Sipahioglu T., Kiraly R., Fesus L, & Telci D. (2020). Role of Tissue Transglutaminase Catalytic and Guanosine Triphosphate-Binding Domains in Renal Cell Carcinoma Progression. ACS Omega, 5(43), 28273-28284.

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Antibody Panel for Adipogenic and Chondrogenic Markers

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Antibodies (Abs) recognizing PPARγ (#2443), GAPDH (#5174), and perilipin (#9349) were purchased from Cell Signaling (Danvers, MA). Antibody against fatty acid binding protein 4 (FABP4) was purchased from ProSci, Inc. (Poway, CA) (XG6174). The anti-adiponectin Ab (PA1-054) was obtained from Affinity BioReagents (Rockford, IL). Aggrecan Ab was from Abcam (ab3778). Antibody against collagen I was purchased from Millipore (AB765P), and the collagen X Ab was purchased from Calbiochem (234,196). For flow cytometry analysis, anti-mouse CD44 (#553131), anti-mouse Sca-1 (#557403), anti-mouse CD105 (#550546), anti-mouse CD106 (#553330), anti-mouse CD90 (#553016), Rat IgG2a control (#559073), were purchased from BD Biosciences (San Jose, CA). Anti-mouse CD146 (#134702), anti-mouse CD73 (#127202), rat IgG2c control (#400702), and rat IgG2b control (#400602) were purchased from Biolegend (San Diego, CA). The mouse monoclonal Ab recognizing the SV40 T antigen (PAb419, sc-58665) was purchased from Santa Cruz Biotechnology (Dallas, TX).

Prideaux M., Wright C.S., Noonan M.L., Yi X., Clinkenbeard E.L., Mevel E., Wheeler J.A., Byers S., Wijenayaka A.R., Gronthos S., Sankar U., White K.E., Atkins G.J, & Thompson W.R. (2021). Generation of two multipotent mesenchymal progenitor cell lines capable of osteogenic, mature osteocyte, adipogenic, and chondrogenic differentiation. Scientific Reports, 11, 22593.

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Multipotent Stromal Cell Phenotyping

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Passage 3–10 MSCs were analyzed for the following markers: CD34, CD45, CD73, CD79, CD90, and CD105. A total of 1 × 10⁵ cells were incubated with each fluorescence-conjugated primary antibody at 37 °C for 2 h in the dark. After three PBS washes, cells were processed by flow cytometry (BD FACSCalibur) and data were analyzed using Cell Quest software. Antibodies used were: anti-mouse CD34, CD45, and CD79 (Santa Cruz Biotechnology Inc.); anti-mouse CD73, CD90, and CD105 (BioLegend, San Diego, CA), and all of the antibodies were labeled with FITC.

Li Y., Xu J., Shi W., Chen C., Shao Y., Zhu L., Lu W, & Han X. (2016). Mesenchymal stromal cell treatment prevents H9N2 avian influenza virus-induced acute lung injury in mice. Stem Cell Research & Therapy, 7, 159.

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