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Ab75014 is a primary antibody product from Cell Signaling Technology. It is a rabbit monoclonal antibody that recognizes a specific target protein. The antibody is designed for use in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunofluorescence.

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Lab products found in correlation

P erk, by Cell Signaling Technology (2 mentions) P akt, by Cell Signaling Technology (2 mentions) Sc 13119, by Santa Cruz Biotechnology (2 mentions) Sc 47778, by Santa Cruz Biotechnology (2 mentions) P yap, by Cell Signaling Technology (2 mentions) Af3629, by Abcam (2 mentions)

2 protocols using ab75014

1

Optimized Western Blot Analysis for Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed according to a previously described standard protocol.49 (link) Primary antibodies were specific for SLIT3 (1:500; R&D Systems, AF3629), ROBO1 (1:500; Abcam, ab7279), ROBO2 (1:1000; Abcam, ab75014), YAP (1:1,000; Cell Signaling, 4912), p-YAP (1:1,000; Cell signaling, 4911), AKT (1:1,000; Cell Signaling, 4691), p-AKT (1:1,000; Cell Signaling, 4060), ERK (1:1,000; Cell Signaling, 9102), p-ERK (1:1,000; Cell Signaling, 9101) and beta-actin (1:5,000; sc-47778, Santa Cruz) or Hsp90 (1:1000; sc-13119, Santa Cruz). Secondary anti-mouse/rabbit HRP-conjugated antibodies were subsequently applied.
Xu R., Yallowitz A., Qin A., Wu Z., Shin D.Y., Kim J.M., Debnath S., Ji G., Bostrom M.P., Yang X., Zhang C., Dong H., Kermani P., Lalani S., Li N., Liu Y., Poulos M.G., Wach A., Zhang Y., Inoue K., Di Lorenzo A., Zhao B., Butler J.M., Shim J.H., Glimcher L.H, & Greenblatt M.B. (2018). Targeting skeletal endothelium to ameliorate bone loss. Nature medicine, 24(6), 823-833.
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2

Optimized Western Blot Analysis for Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed according to a previously described standard protocol.49 (link) Primary antibodies were specific for SLIT3 (1:500; R&D Systems, AF3629), ROBO1 (1:500; Abcam, ab7279), ROBO2 (1:1000; Abcam, ab75014), YAP (1:1,000; Cell Signaling, 4912), p-YAP (1:1,000; Cell signaling, 4911), AKT (1:1,000; Cell Signaling, 4691), p-AKT (1:1,000; Cell Signaling, 4060), ERK (1:1,000; Cell Signaling, 9102), p-ERK (1:1,000; Cell Signaling, 9101) and beta-actin (1:5,000; sc-47778, Santa Cruz) or Hsp90 (1:1000; sc-13119, Santa Cruz). Secondary anti-mouse/rabbit HRP-conjugated antibodies were subsequently applied.
Xu R., Yallowitz A., Qin A., Wu Z., Shin D.Y., Kim J.M., Debnath S., Ji G., Bostrom M.P., Yang X., Zhang C., Dong H., Kermani P., Lalani S., Li N., Liu Y., Poulos M.G., Wach A., Zhang Y., Inoue K., Di Lorenzo A., Zhao B., Butler J.M., Shim J.H., Glimcher L.H, & Greenblatt M.B. (2018). Targeting skeletal endothelium to ameliorate bone loss. Nature medicine, 24(6), 823-833.
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